OriGene Logo

2010111I01Rik (NM_028079) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG215648
2010111I01Rik (tGFP-tagged) - Mouse RIKEN cDNA 2010111I01 gene (2010111I01Rik), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $954.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8
100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "2010111I01Rik or NM_028079"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 2010111I01Rik
Synonyms 2300006M17Rik; Aopep; AP-O
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215648 representing NM_028079
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACATAAAGCTGGACCCTTCCCGGGATGATCTGCCTCTCATGGCCAACACCAGCCACATGCTTGTGA
AGCACTACATACTGGATTTGGATGTGGACTTTGGAAATCAAGTCATTGAGGGCAACATAGTGCTTTTCTT
TGGAGATGGAAACAGATTTAAAAACCAGTCGCGTTCTACCCAGGAAACCTTCCAGATGGAGTCAGAGGAG
GCTGACATATTTAGGACAGCTGAACCCTGCCATGTTCCTGAGATGGATTCGAGTACCTTCTCACCTAAGA
TGGGACATAGAGAATGTGCAGTCTGTGGTAAAGGTGATCAAGATGCCTTTGATAACGATGGTAACCATGA
CAACCAGGAACGTGATTCTGAGATCTCTAGCTCAAAGTACTGCTGTGACACAGGGAATCATGGGAAAAGG
GATTTCTTGCTAGTGTTGGACTGCTGTGATTTATCTGTGCTAAAGGTAGAGGAGGTGGATGTGGCTGCTG
TGCCAGGCCTTGAGAAATTTACTAAAGCTCCAAAGCTCTTGGCTACTCCTGAGAAGCTCAGGTGTGAGAT
TGTCCGTGACCTTGTGGCTCTGCCTGCAGATGCTTGGAGGGAGCAGTTAGACTGCTACACTCGTTGCAGC
CAGGCTCCTGGCTGTGGGGAGCTCTTGATTGACTCTGACAATTGGAGCTTACGGATCAGGAAGACGGGGA
CTTCGACACCTGCTGACTTTCCTCGTGCCATCAGAATATGGTATAAAACTAAACCTGAGGGGCAGTCAGT
AGCTTGGACAACAGACCAGAATGGCAGGCCATGTGTTTATACTATGGGATCCCCCATCAACAACAGGGCC
CTTTTTCCATGCCAGGAACCACCCGTTGCCATGTCAACATGGCAGGCTACAGTTCGAGCAGCTGCATCTT
TTGTTGTTTTAATGAGTGGAGAAAATTCTGCCAAGCCCACACCACTTCGAGAAGGATACATGAGCTGGCA
TTACTATGTGACCATGCCAATGCCGGCCTCTACCTTTGCAATTGCAGTGGGGTGCTGGACAGAAATGAAG
CCCAAGGCATCCCCACCAGATGATCTGATGACTGAGCACTCTCTCCCCCTCTCGCCATCGGAAGCGGACT
TGAGGTATGATAACACCTGCAATCATATGGAATACCCTTGCAGGTTCCAGAGTGCGTCCGCTGCCTCCCA
GGACATCATTCCTTATCGAGTGTTTGCCCCAGTGTGCCTTGAGGGTGCCTGCCAAGAGGCCCTGCTGTGG
CTGATCCCTTCTTGCCTCTCAGCTGCACACTCTGTCCTGGGAACACACCCTTTCTCCCGGCTGGACATAC
TCATTGTCCCCACCAACTTTCCAAGTCTGGGAATGGCCAGCCCACACATCATCTTCCTCTCTCAGAGCAC
CTTAACAGGCACGAGCCATCTCTGTGGGACCCGTCTTTGCCATGAAATTGCTCACTCCTGGTTTGGCCTA
GCCATTGGGGCCCGAGACTGGACAGAGGAGTGGCTCAGTGAAGGATTTGCCACGCATTTGGAAGATATAT
TTTGGGCTGAGGCACAGCAGCTGCCCCCCCATGAGGCCCTGGAGCAGCAGGAGCTGAGGGCTTGCCTGCG
CTGGCACCGCCTGCAGGATGAGCTTCGGAACTCCCCAGAGGGAATGCAGGTGTTAAGACCCAACAAAGAA
GAGACTGGCCATGTGAGTGCTTCAGGTGCATCTGTTGTCAAGCATGGACTCAATCCAGAGAAGGGCTTCA
TGCAGGTTCATTACTTAAAGGGCTACTTCCTTCTTCGGTTCCTAACCAGAACACTTGGAGAGAAAATTTA
TTTTCCGTTTTTAAGAAAATTTGTGCATCTGTTTCATGGGCAGCTGATTCTTTCCCAGGATTTTCTTCAA
ATGCTGTTGGAGAACATTCCAGAAAACAAAAGGCTCGGCCTGTCTGTTGAGAACATCGTCCGAGATTGGC
TTGAGTGTTCCGGAATACCTAAGGCGCTGCAGGAGGAGCGCAAGGCCGAGGACTGCTCGCCGAGTAGGCT
CGCACGGCAAGTAGGCTCCGAGGTGGCGAAATGGATTCGAGTCAACCGCAGACCCCGAAAACGCAAACGA
GGGAAGCGAGAAGTCGCCTTTGAAAAGCTTTCTCCAGACCAGATCGTCTTGCTTTTGGAGTGGCTCTTAG
AGCAGAAGACGCTGAGCCCTCAGACACTGCACTGTCTCCAGCAGACTTACCATCTCCCAGAGCAGGATGC
AGAGGTTCGCCATCGATGGTGTGAACTGGTTATTAAGCACAAGTACACAAAGGCATACAATCAGGTGGAG
AGGTTCCTACTGGAGGACCAGGCCATGGGCATATACCTGTATGGGGAGCTGATGGTGAGTGAGGACGCCA
GGCTGCAGCAGCTAGCCCACAGGTGCTTTGAGCTGGTGAAGGAACACATGGACAGAGCATCGGCCCAGGT
GGTGACTGAAATGCTGTTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215648 representing NM_028079
Red=Cloning site Green=Tags(s)

MDIKLDPSRDDLPLMANTSHMLVKHYILDLDVDFGNQVIEGNIVLFFGDGNRFKNQSRSTQETFQMESEE
ADIFRTAEPCHVPEMDSSTFSPKMGHRECAVCGKGDQDAFDNDGNHDNQERDSEISSSKYCCDTGNHGKR
DFLLVLDCCDLSVLKVEEVDVAAVPGLEKFTKAPKLLATPEKLRCEIVRDLVALPADAWREQLDCYTRCS
QAPGCGELLIDSDNWSLRIRKTGTSTPADFPRAIRIWYKTKPEGQSVAWTTDQNGRPCVYTMGSPINNRA
LFPCQEPPVAMSTWQATVRAAASFVVLMSGENSAKPTPLREGYMSWHYYVTMPMPASTFAIAVGCWTEMK
PKASPPDDLMTEHSLPLSPSEADLRYDNTCNHMEYPCRFQSASAASQDIIPYRVFAPVCLEGACQEALLW
LIPSCLSAAHSVLGTHPFSRLDILIVPTNFPSLGMASPHIIFLSQSTLTGTSHLCGTRLCHEIAHSWFGL
AIGARDWTEEWLSEGFATHLEDIFWAEAQQLPPHEALEQQELRACLRWHRLQDELRNSPEGMQVLRPNKE
ETGHVSASGASVVKHGLNPEKGFMQVHYLKGYFLLRFLTRTLGEKIYFPFLRKFVHLFHGQLILSQDFLQ
MLLENIPENKRLGLSVENIVRDWLECSGIPKALQEERKAEDCSPSRLARQVGSEVAKWIRVNRRPRKRKR
GKREVAFEKLSPDQIVLLLEWLLEQKTLSPQTLHCLQQTYHLPEQDAEVRHRWCELVIKHKYTKAYNQVE
RFLLEDQAMGIYLYGELMVSEDARLQQLAHRCFELVKEHMDRASAQVVTEMLF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028079
ORF Size 2469 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028079.2, NP_082355.1
RefSeq Size 3509 bp
RefSeq ORF 2472 bp
Locus ID 72061
Cytogenetics 13 B3
Summary Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing of bioactive peptides in tissues such as testis and heart.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_028079" in other vectors (4)
SKU Description Size Price
MC221877 2010111I01Rik (untagged) - Mouse RIKEN cDNA 2010111I01 gene (2010111I01Rik), (10ug) 10 ug
$830.00
MR215648 2010111I01Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2010111I01 gene (2010111I01Rik) 10 ug
$589.00 $754.00
MR215648L3 Lenti ORF clone of 2010111I01Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2010111I01 gene (2010111I01Rik) 10 ug
$1,054.00
MR215648L4 Lenti ORF clone of 2010111I01Rik (mGFP-tagged) - Mouse RIKEN cDNA 2010111I01 gene (2010111I01Rik) 10 ug
$1,054.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.