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Fgd3 (NM_015759) Mouse Tagged ORF Clone

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SKU
MG215633
Fgd3 (tGFP-tagged) - Mouse FYVE RhoGEF and PH domain containing 3 (Fgd3), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $871.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fgd3
Synonyms 5830461L01Rik; ZFYVE5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215633 representing NM_015759
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATTGGGTAGGAGCTCCTCGACACCCCAAGAAGAAGCCATTTCCCCTCTTGGAGTACTTGGCACTG
GGCCCAGCAGCTCCCCACTAGGGAAGCTTCAAGCCCTTCCCATCGGTCCAGGTGCCCACCGTGGGGCTCA
TAGCAGCTCAGCCCCAGCAGGGGACAGCTCCACGCGGGAGCCAAGTGGTGCCATGAAGATCCCCAACAGA
GACAGTGGAATTGACAGCCCATCCTCCAGTGTGGCCAGTGAGAACTTCCCCTGTGAGGAGAGCAGTGAGG
GCTCTCCAAGCCCTGCCATCCTGGGGCTACCCTCTGAGACGGCTTCGGACAGCCGGGTCCCACAGGACAA
CCCCCAGGAGGAAGAAGATAGTGGCGTGGGTGAGGAGCCTGACCCCAAGGTCACCCTGTTTAGGCCTCAG
GAGGATGTCAGCTTGACTCAGTGCTCTGACCCACAGAAGCTGCTTCATATTGCCCAGGAGCTCCTGCACA
CGGAGGAAGCCTACGTGAAGCGGCTGCACCTGCTGGACCAGGTTTTCTGTACTAAGCTGACAGAAGCAGG
GATCCCTCTGGAAGTCACCACCGGCATCTTCTCGAACATCTCTTCCATCTACCGTTTCCATGGGCAGTTC
CTCCTGCCCGAGCTGCAGAAGAGGATCACAGAGGAGTGGGATACAAACCCTCGCCTCGGAGACATCCTGC
AGAAACTGGCTCCATTCCTGAAGATGTATGGCGAGTACGTCAAGAACTTTGACAGAGCGATGGGGCTGGT
GAGCACTTGGACCCAGCGTTCTCCGCAGTTTAAGGATGTCATCCACACCATCCAGAAGCAGGAGGTGTGT
GGGAACTTGACACTGCAGCATCACATGCTGGAGCCTGTGCAGAGGGTCCCCCGCTATGAGCTGCTGCTCA
AGGACTACCTAAAGAGGCTCCCCAGGGATGCCCCAGACCGGAAGGATGCAGAGAGGTCCCTGGAGCTCAT
CTCCACAGCTGCTGACCACTCCAATGCTGCCATTAGGAAGATGGAGAAAATGCACAAGCTCTTAGAGGTG
TATGAGCAGCTGGGTGGGGAAGAGGACATCGTTAACCCAGCCAATGAGCTGATCAAGGAGGGCAGCATCC
AGAAGTTATCAGCCAAGAATGGCACCACTCAGGACCGACACCTCTTCCTGTTCAACAATGTGATGCTCTA
CTGTGTACCCAAACTGAGGCTCATGGGCCAGAAGCTAAGTGTACGGGAAAAAATGGACATCTCTGATCTC
CAGGTACAAGATATTGTGAAACCAAACGCAGCCTGCACGTTCATCATAACAGGAAGAAAAAGGTCCCTGG
AGCTTCAGACTCGGACAGAAGAAGAGAAGAAAGAATGGATTCAGGTCATTCAGGCCACTGTGGAGAAGCA
CAAACAGAAGAGTGAGACCTTCAGGGCCTTTGGCGGTGCCTGTAGCCAGGATGAGGAGCCCACCCTGTCT
CCAGACCAGCCCGTCATGAGTACCAGCTCTGTGGAACCTGCAGGGGTAGCTGACAGCAATGGGGGTACTC
CCGGGATCGAGTCCAGAAAGTCGTCCTCTAAAACAAGACGCGACAAAGAGAAGCCCGGGTGTAAGAGCTG
TGGTGAGACCTTCAACTCCATCACTAAGAGGAGGTATCGCTGCAAGCTGTGTGGAGAGGTCATCTGTAGG
AAGTGCTCAGAGTTCAAGGCAGAGAACAGTAAGCAGAGCCGCGTCTGCCGAGAGTGTTTCCTGGAAGAGC
CCCTGGTCCCTCCAAGCCCCAGCTCTGAGACTCCCACTGAGCTCAAACAGAATGCAGAGAAGCCTCCCTC
TGTAGATCCTCGGCCCAGCCTGCTCTGCGGTACCCTGAATCTGTCAGACGATGGTACGACCTGGAATGAA
GTGTGGGCAGCCATCCCTGAATCAGATCCTCAGGTGCTAGACCTGCTGGCAGGCAGCCAGGCTGGCAGAC
TGCTGTATAGCATCCCACTCTCTGGCTGTAATATAACGATGCCTGACCCTGAGGAGGGGCTGGAGGCAGG
ATGTGCCTGGAAGCTACATCAGGGCTCACAGACCTGGTGGCTGAGCGCCCCCTCCACAAAGCTGCAGCAA
TGCTGGCTGAAAGCCCTGGGCACTGCCGTACATGGGGACACAGCCGGGGACAGACCAGGGGCCTCCCAGC
CCCAGGCCCCAGCAGGCACGGACACTCCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215633 representing NM_015759
Red=Cloning site Green=Tags(s)

MELGRSSSTPQEEAISPLGVLGTGPSSSPLGKLQALPIGPGAHRGAHSSSAPAGDSSTREPSGAMKIPNR
DSGIDSPSSSVASENFPCEESSEGSPSPAILGLPSETASDSRVPQDNPQEEEDSGVGEEPDPKVTLFRPQ
EDVSLTQCSDPQKLLHIAQELLHTEEAYVKRLHLLDQVFCTKLTEAGIPLEVTTGIFSNISSIYRFHGQF
LLPELQKRITEEWDTNPRLGDILQKLAPFLKMYGEYVKNFDRAMGLVSTWTQRSPQFKDVIHTIQKQEVC
GNLTLQHHMLEPVQRVPRYELLLKDYLKRLPRDAPDRKDAERSLELISTAADHSNAAIRKMEKMHKLLEV
YEQLGGEEDIVNPANELIKEGSIQKLSAKNGTTQDRHLFLFNNVMLYCVPKLRLMGQKLSVREKMDISDL
QVQDIVKPNAACTFIITGRKRSLELQTRTEEEKKEWIQVIQATVEKHKQKSETFRAFGGACSQDEEPTLS
PDQPVMSTSSVEPAGVADSNGGTPGIESRKSSSKTRRDKEKPGCKSCGETFNSITKRRYRCKLCGEVICR
KCSEFKAENSKQSRVCRECFLEEPLVPPSPSSETPTELKQNAEKPPSVDPRPSLLCGTLNLSDDGTTWNE
VWAAIPESDPQVLDLLAGSQAGRLLYSIPLSGCNITMPDPEEGLEAGCAWKLHQGSQTWWLSAPSTKLQQ
CWLKALGTAVHGDTAGDRPGASQPQAPAGTDTP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015759
ORF Size 2199 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015759.2, NP_056574.2
RefSeq Size 3110 bp
RefSeq ORF 2202 bp
Locus ID 30938
Cytogenetics 13 25.18 cM
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Documents
Resources
"NM_015759" in other vectors (4)
SKU Description Size Price
MC221166 Fgd3 (untagged) - Mouse FYVE, RhoGEF and PH domain containing 3 (Fgd3), (10ug) 10 ug
$739.00
MR215633 Fgd3 (Myc-DDK-tagged) - Mouse FYVE, RhoGEF and PH domain containing 3 (Fgd3) 10 ug
$589.00 $671.00
MR215633L3 Lenti ORF clone of Fgd3 (Myc-DDK-tagged) - Mouse FYVE, RhoGEF and PH domain containing 3 (Fgd3) 10 ug
$971.00
MR215633L4 Lenti ORF clone of Fgd3 (mGFP-tagged) - Mouse FYVE, RhoGEF and PH domain containing 3 (Fgd3) 10 ug
$971.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.