OriGene Logo

Mcm3 (NM_008563) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG215631
Mcm3 (tGFP-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $1,018.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8
100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "Mcm3 or NM_008563"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mcm3
Synonyms AL033361; C80350; Mcmd; P1; P1-MCM3; p1.m
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215631 representing NM_008563
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCACAGTAGTGCTGGATGATGTGGAGCTGCGGGAAGCGCAGAGAGACTACTTGGACTTCCTGG
ATGACGAGGAGGACCAAGGCATTTACCAGAACAAGGTTCGGGAACTGATCAGTGACAATCAGTATCGGCT
GATTGTCAGCGTGAATGACCTGCGCAGGAAGAATGAAAAGAGGGCTAACCGCCTCCTGAACAATGCGTTT
GAGGAGCTGGTTGCCTTCCAGCGGGCCTTGAAGGATTTCGTGGCCTCCATTGACGCCACCTACGCCAAGC
AGTATGAGGAGTTCTACATAGGATTGGAAGGCAGCTTTGGCTCAAAGCACGTCTCTCCCCGGACTCTCAC
TTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAAGGCATTGTTACTAAATGCTCTCTGGTTCGCCCCAAA
GTCGTTCGCAGTGTTCACTACTGTCCTGCTACTAAGAAGACCATAGAGCGACGCTACTCTGACCTCACCA
CCCTAGTGGCTTTCCCATCCAGTTCTGTTTATCCCACAAAGGATGAGGAGAACAACCCCCTGGAGACAGA
GTATGGCCTGTCTGTCTACAAGGACCATCAGACCATCACCATCCAGGAGATGCCAGAGAAGGCCCCTGCT
GGTCAGCTCCCTCGCTCTGTGGATGTCATTCTAGACGATGACCTGGTGGATAAAGTGAAGCCTGGTGACA
GAATACAGGTGGTGGGGACCTACCGCTGCCTTCCAGGGAAGAAGGGCTGCTACACCTCGGGGACCTTCAG
GACCGTCCTGATTGCCTGTAATGTGAAGCAGATGAGTAAGGATATTCAGCCTGCATTCTCTGCGGACGAT
ATAGCCAAGATCAAGAAGTTCAGCAAAACTCGTTCCAAGGATGTCTTTGAGCAGCTGGCCCGGTCGTTAG
CCCCGAGCATCCATGGGCATGACTATGTCAAGAAGGCAATCCTCTGCTTGCTCCTGGGAGGGGTGGAGCG
GGAACTTGAAAACGGCAGCCACATCCGTGGAGACATCAATATTCTTCTAATAGGTGACCCCTCCGTTGCC
AAGTCCCAGCTTCTGCGGTACGTGCTTTGCACGGCGCCCAGGGCCATTCCCACCACGGGACGGGGCTCCT
CTGGAGTGGGTCTCACAGCTGCTGTCACTACAGACCAGGAAACAGGGGAGCGCCGCCTGGAAGCTGGTGC
TATGGTCCTGGCTGACCGTGGAGTGGTTTGCATTGATGAGTTTGACAAGATGTCTGACATGGACCGCACA
GCCATCCACGAGGTGATGGAGCAGGGTCGAGTGACCATTGCAAAGGCTGGCATCCATGCCCGGCTCAATG
CCCGCTGCAGTGTTTTGGCAGCTGCCAATCCAGTCTATGGCAGGTATGATCAGTATAAGACGCCCATGGA
GAACATTGGGCTGCAGGACTCACTGCTGTCTAGATTTGACCTGCTCTTCATCATGCTGGATCAGATGGAT
CCTGAACAGGATCGGGAGATCTCAGACCACGTCCTGAGGATGCACCAATACAGGGCCCCGGGGGAGCAGG
ACGGTGATGCTTTGCCATTGGGTAGTTCAGTGGATATCCTGGCCACAGACGATCCTGACTTTACCCAGGA
TGACCAGCAAGATACCCGCATTTATGAGAAGCATGACAGCCTTCTACATGGGACCAAGAAGAAAAAGGAG
AAGATGGTGAGCGCAGCTTTTATGAAGAAGTACATTCACGTGGCCAAAATTATCAAGCCCACCCTGACAC
AGGAGTCAGCAGCCTACATTGCAGAAGAATACTCACGCCTGCGCAGCCAGGACAGTATGAGCTCAGACAC
TGCCCGGACATCTCCAGTTACAGCACGGACACTGGAAACTCTGATTCGACTGGCCACGGCCCATGCGAAG
GCCCGGATGAGCAAGACTGTGGACCTGCAGGATGCAGAGGAAGCCGTGGAGCTGGTTCAGTATGCTTATT
TCAAGAAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGGCAAGTGAGGATGAATCAGACCTAGAAGATGA
AGAGGAAAAGAGCCAGGAAGACACTGAGCAGAAGCGGAAAAGGAGGAAGACTCATGCCAAGGATGGAGAG
TCCTATGACCCTTACGACTTCAGTGAAGCAGAGACGCAGATGCCTCAAGTGCACACCCCAAAGACTGACG
ATTCCCAAGAGAAGACCGATGATTCTCAGGAGACCCAGGACTCCCAGAAAGTGGAACTCAGTGAGCCCAG
GCTGAAGGCTTTTAAGGCGGCCCTCTTAGAAGTGTTCCAGGAAGCTCATGAGCAGTCTGTGGGCATGCTC
CACCTCACAGAATCCATCAACCGGAATAGAGAAGAGCCGTTCTCCTCAGAGGAAATCCAGGCTTGCCTGA
GCCGGATGCAGGATGATAACCAGGTCATGGTGTCTGAGGGCATCGTCTTCCTTATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215631 representing NM_008563
Red=Cloning site Green=Tags(s)

MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQNKVRELISDNQYRLIVSVNDLRRKNEKRANRLLNNAF
EELVAFQRALKDFVASIDATYAKQYEEFYIGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPK
VVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPA
GQLPRSVDVILDDDLVDKVKPGDRIQVVGTYRCLPGKKGCYTSGTFRTVLIACNVKQMSKDIQPAFSADD
IAKIKKFSKTRSKDVFEQLARSLAPSIHGHDYVKKAILCLLLGGVERELENGSHIRGDINILLIGDPSVA
KSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRT
AIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMD
PEQDREISDHVLRMHQYRAPGEQDGDALPLGSSVDILATDDPDFTQDDQQDTRIYEKHDSLLHGTKKKKE
KMVSAAFMKKYIHVAKIIKPTLTQESAAYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAK
ARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKASEDESDLEDEEEKSQEDTEQKRKRRKTHAKDGE
SYDPYDFSEAETQMPQVHTPKTDDSQEKTDDSQETQDSQKVELSEPRLKAFKAALLEVFQEAHEQSVGML
HLTESINRNREEPFSSEEIQACLSRMQDDNQVMVSEGIVFLI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008563
ORF Size 2436 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008563.3
RefSeq Size 2886 bp
RefSeq ORF 2439 bp
Locus ID 17215
UniProt ID P25206
Cytogenetics 1 A4
Summary Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_008563" in other vectors (4)
SKU Description Size Price
MC205531 Mcm3 (untagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3), (10ug) 10 ug
$744.00
MR215631 Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3) 10 ug
$818.00
MR215631L3 Lenti ORF clone of Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3) 10 ug
$1,118.00
MR215631L4 Lenti ORF clone of Mcm3 (mGFP-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3) 10 ug
$1,118.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.