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Cast (NM_009817) Mouse Tagged ORF Clone

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SKU
MG215608
Cast (tGFP-tagged) - Mouse calpastatin (Cast), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$789.00 $959.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cast
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215608 representing NM_009817
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATTCGCAAGTTGGTGGTACAAGACGCATGTTAATGAAAAGAATATTGGATCATCTTCTAAACCAG
GAGAAAAGAAAGGATCCGATGAGAAAAAAGCAGCAAGTTTGGGGAGCAGTCAGCCTTCCAGACCACATGT
TGGTGAAGCTGCCACAGCCACCAAGGTGACAGCTTCCTCTGCTGCCACCAGCAAGTCCCCCAGCATGAGT
ACCACAGAGACTAAGGCTGTGAAAACAGAGTCTAAGAAACCACAATCAAGTGAGCAACCTGTGGTTCACG
AGAAAAAGTCAAAAGGAGGGCCAAAGGAAGGGTCAGAGCCCAAAAATCTTCCGAAGCACACATCAAGTAC
AGGAAGCAAGCATGCTCATAAAGAAAAAGCACTTTCCAGATCAAATGAACAGATGGTGTCAGAGAAACCA
TCAGAATCAAAGACAAAATTTCAAGATGTTCCTTCAGCTGGTGGAGAGAGTGTGGCTGGAGGTGGGACTG
TGGCCACTGCCTTGGACAAAGTGGTTGGTAAGAAAAAAGAACAGAAACCATTCACACCAGCTTCACCTGT
GCAGTCCACACCGAGCAAGCCATCTGACAAGTCAGGCATGGATGCTGCCTTGGATGACCTGATAGACACC
TTAGGTGGACATGAAGATACTAACAGAGATGATCCACCATATACTGGACCAGTAGTTTTGGACCCAATGT
ATTCTACCTACCTTGAGGCACTGGGTATAAAAGAAGGGACTATTCCTCCAGAGTATAGGAAACTTTTGGA
AAAAAATGAAGGGATCACACAACCTCTTCCAGACTCTCCTAAACCTATGGGGACTGACCAGGCTATAGAT
GCCTTGTCATCTGATTTCACCTGTAGTTCTCCAACTGGAAAGCAAAGTGAGAAAGAGAAGTCTACTGGGG
AAATTTTCAAAGCTCAGTCTGCAGGAGTGACCAGAAGTTCTGTTCCCCCCAAGGAGAAGAAAAGGAAGGT
GGAAGAGGAGGTCATAAATGATCAAGCACTTCAGGCTCTGTCAGATTCACTGGGCACCCGGCAGCCAGAT
CCTCCGAGCCACGTTAGCCAAGCTGAACAAGTCAAAGAGGCAAAAGCAAAAGAAGAAAGGCAGGAGAAGT
GTGGTGAAGATGAGGACACAGTCCCAGCTGAGTACAGGTTAAAACCAGCAAAGGATAAAGATGGAAAACC
ACTATTGCCAGAGCCTGAAGAAACATCTAAGAGCCTGAGTGAGTCGGAGCTGATTGGGGAGCTTTCAGCA
GATTTTGACCGATCTACATATCAAGACAAACCATCTACGCCAGCTGAAAAGAAATCCAATGACACATCCC
AAACTCCTCCGGGGGAGACTGTGCCTCGGGCCTCCATGTGCAGTATACGGTCAGCGCCACCCAAACTAGC
ATCCTTGAAGGGCGTGGTACCAGAAGATGCTGTTGAAACCTTGGCTGGAAGCCTGGGGACAAGGGAAGCA
GATCCAGAACATGAAAAAACTGTGGAGGATAAAGTCAAGGAGAAAGCTAAAGAAGAAGAGCATGAAAAAC
TTGGTGAAAAAGAAGAAACAGTGCCTCCTGATTATCGACTAGAAGAAGTCAAGGATAAGGATGGAAAACC
ACTCCTGCCCAAAGAATCCCAGGAACAACTTGCACCCTTAAGCGATGACTTCCTTCTTGATGCCTTGTCT
CAGGACTTCTCCAGTCCGGCAAACATATCGTCTCTTGAATTTGAAGATGCCAAACTTTCTGCTGCCATTT
CTGAAGTAGTTTCTCAGACACCTGCTCCAAGCACCCATGCAGCAGCTCCACTGCCTGGCACTGAGCAGAA
AGACAAAGAACTTGATGATGCCTTGGATGAACTTTCTGACAGTCTTGGACAAAGGCCGCCTGATCCAGAT
GAGAACAAACCACTGGATGACAAAGTGAAGGAGAAAATCAAACCAGAGCATAGCGAGAAACTGGGAGAAA
GAGACGACACCATCCCCCCTGAATACAGGCATCTCTTGGATAATGATGGGAAGGACAAACCAGAGAAGCC
ACCGACTAAGAAAACAGAGAAACCTGATCAGGACCGGGACCCCATTGATGCCCTCTCAGAAGATTTGGAT
AGCTGCCCCTCAACTACAGAGACCTCAAAGAATACAGCAAAGGGGAAGAGCAAGAAGACTTCAAGTTCCA
AAGCATCCAAGGACGGAGAGAAAACAAAGGACTCTTCCAAGAAGACAGAGGAAGTGTCCAAGCCAAAGGC
TAAAGAAGATGCAAGACACAGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215608 representing NM_009817
Red=Cloning site Green=Tags(s)

MAFASWWYKTHVNEKNIGSSSKPGEKKGSDEKKAASLGSSQPSRPHVGEAATATKVTASSAATSKSPSMS
TTETKAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKP
SESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDT
LGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAID
ALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPD
PPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSA
DFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREA
DPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALS
QDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPD
ENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLD
SCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009817
ORF Size 2262 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009817.4
RefSeq Size 2894 bp
RefSeq ORF 2265 bp
Locus ID 12380
Cytogenetics 13 C1
Summary This gene encodes an inhibitor of the calcium-dependent cysteine protease, calpain. This protein plays roles in multiple processes, including apoptosis, cell cycle regulation, and membrane fusion. Multiple protein isoforms exist which contain unique N-terminal domains, and multiple inhibitory domains that share homology with each other. Some isoforms may be tissue-specific. Two different pseudogenes of this gene are found on chromosome 19. provided by RefSeq, Jul 2014
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Documents
Resources
"NM_009817" in other vectors (1)
SKU Description Size Price
MR215608 Cast (Myc-DDK-tagged) - Mouse calpastatin (Cast) 10 ug
$759.00

file-alt Citations

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