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Map3k10 (NM_001081292) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215590
Map3k10 (tGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase 10 (Map3k10), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,062.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Map3k10
Synonyms BC028668; BC046514; Mlk2; MST
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215590 representing NM_001081292
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGGAGGAGGGGGCGGCGGCCAGAGAGTGGGGCGCGACCCCCGCGGGGCCAGTGTGGACCGCCG
TGTTCGACTACGAGGCGGTGGGCGACGAGGAGCTGACCCTGCGGAGAGGCGACCGCGTCCAGGTGCTTTC
CCAGGACTGTGCGGTGTCTGGAGATGAGGGCTGGTGGACAGGGCAGCTGCCCAGCGGCCGTGTGGGCGTC
TTCCCCAGCAACTACGTGGCCCCCGCTGCACCCGCCGCACCCTCAGACCTCCAGCTGCCACAGGAGATCC
CCTTCCACGAGCTGCAGCTGGAGGAGATCATCGGTGTCGGGGGCTTTGGCAAAGTCTATCGCGCCGTATG
GCGTGGAGAGGAAGTAGCGGTCAAGGCCGCTCGGCTGGACCCTGAACGGGACCCGGCAGTGACAGCGGAG
CAGGTCCGCCAGGAGGCCCGGCTCTTTGGAGCCCTGCAGCACCCCAACATCATTGCCCTGAGGGGCGCCT
GCCTCAGCCCCCCAAACCTCTGCCTGGTGATGGAATATGCTCGGGGAGGTGCATTGAGCAGGGTACTGGC
AGGTCGCCGGGTCCCCCCTCATGTGCTGGTCAACTGGGCTGTGCAGGTGGCTCGGGGCATGAACTATCTA
CACAATGATGCCCCTGTGCCCATCATTCACCGGGACCTCAAGTCCATCAACATCCTAATCCTGGAGGCCA
TTGAGAACCACAACCTCGCTGACACGGTGCTCAAGATCACAGACTTCGGCCTTGCCCGAGAGTGGCACAA
GACGACCAAAATGAGTGCAGCGGGGACCTACGCCTGGATGGCTCCTGAGGTTATCCGCCTGTCCCTCTTC
TCCAAAAGCAGCGACGTCTGGAGCTTCGGGGTTCTTCTTTGGGAGCTGCTGACCGGGGAGGTCCCCTACC
GAGAAATCGACGCCTTGGCCGTGGCTTACGGTGTGGCTATGAACAAGCTGACGCTACCCATCCCCTCCAC
GTGCCCCGAGCCCTTTGCCCGCCTACTGGAGGAATGCTGGGACCCAGACCCTCACGGGCGACCAGATTTT
GGCAGCATCCTGAAGCAGCTGGAGGTCATCGAGCAGTCAGCCCTGTTCCAGATGCCACTGGAGTCCTTCC
ACTCCCTGCAGGAAGACTGGAAGCTGGAGATTCAGCATATGTTTGATGACCTTCGGACCAAGGAGAAGGA
GTTGCGCAGCCGGGAAGAGGAGCTATTGCGGGCTGCACAGGAGCAGCGTTTTCAGGAAGAGCAGCTACGG
CGTCGGGAGCAGGAGCTGGCAGAGCGAGAGATGGACATCGTAGAACGTGAGCTGCACCTGCTCATGAGCC
AGCTGAGCCAGGAGAAGCCCAGGGTCCGAAAACGCAAGGGCAACTTCAAGCGCAGCCGCTTGCTCAAGCT
ACGGGAAGGAAGCAGCCACATCAGCCTGCCCTCTGGATTTGAACATAAGATCACCGTCCAGGCCTCCCCA
ACCCTGGACAAGAGGAAAGGATCCGATGGGGCCAGCCCTCCTGCCAGCCCCAGCATCATTCCCCGGTTGA
GGGCCATTCGATTGACTCCCATGGACTGTGGTGGCAGCAGTGGTAGTGGAACATGGAGCCGCAGTGGGCC
ACCAAAGAAGGAAGAACTGGTTGGGGGCAAGAAGAAAGGCAGGACATGGGGCCCCAGTTCCACTTTGCAG
AAGGAGCGAGCTGGAGGAGAGGAGAGGCTCAAGGCCCTGGGGGAAGGAAGCAAGCAGTGGTCCTCGAGCG
CCCCCAACCTGGGCAAATCCCCTAAGCACACCCCCATGGCCCCAGGCTTCGCCAGCCTCAACGAGATGGA
AGAATTTGCGGAGGCAGATGAAGGCAACAACGTGCCCCCTTCTCCCTACTCCACCCCGTCCTACCTCAAG
GTGCCTCTGCCCGCCGAGCCTTCTCCTTGTGTTCAGGCGCCTTGGGAGCCGCCGGCTGTCACCCCTTCCC
GGCCAGGACACGGAGCTCGTAGGCGCTGCGACCTGGCGCTGCTAAGCTGTGCCACGCTGCTGAGTGCGGT
GGGCCTGGGCGCCGACGTGGCCGAGGCACGGGCCGGGGACGGTGAGGAGCAGCGGCGCTGGCTGGACAGT
CTCTTCTTCCCGCGCCCCGGCCGCTTCCCTCGCGGTCTCAGCCCAACCGGGCGCCCCGGAGGTCGCCGCG
AAGACACAGCCCCAGGCTTGGGTCTGGCACCCTCGGCCACCCTGGTGTCATTATCCTCTGTATCGGATTG
TAACTCTACGCGCTCATTGCTGCGCTCTGACAGTGACGAGGCTGCCCCTGCCGCCCCCTCCCCGCCGCCT
TCTCCGCTCGCGCCCTCACCCAGCACCAATCCTCTCGTGGACGTGGAGTTGGAGAGCTTCAAGAAGGACC
CCCGGCAGTCACTTACGCCCACCCACGTCACTGCCGCCCACGCTGTGAGCCGTGGACACAGGCGGACGCC
ATCAGATGGGGCTTTGAGACAACGGGAGCCTCTGGAGCTCACCAACCACGGACAGGGTCCTCGAGACCCC
CTGGACTTCCCTCGACTGCCCGACCCCCAGGCCCTGTTCCCTACCCGACGCCGGCCCCTGGAGTTCCCTG
GCCGCCCCACCACCCTCACTTTTGCCCCAAGACCCCGGCCTGCTGCCAGCCGCCCCCGTCTGGATCCCTG
GAAACTGGTCTCCTTTGGCCGGACACTCAGCATCTCGCCTCCAAGCAGGCCAGACACTCCAGAGAGCCCT
GGGCCCCCCAGCGTGCAACCCACGTTGCTGGACATGGACATGGAGGGCCAGAGCCAGGACAATACAGTGC
CCCTATGTGGGGTCTATGGCTCTCAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215590 representing NM_001081292
Red=Cloning site Green=Tags(s)

MEEEEGAAAREWGATPAGPVWTAVFDYEAVGDEELTLRRGDRVQVLSQDCAVSGDEGWWTGQLPSGRVGV
FPSNYVAPAAPAAPSDLQLPQEIPFHELQLEEIIGVGGFGKVYRAVWRGEEVAVKAARLDPERDPAVTAE
QVRQEARLFGALQHPNIIALRGACLSPPNLCLVMEYARGGALSRVLAGRRVPPHVLVNWAVQVARGMNYL
HNDAPVPIIHRDLKSINILILEAIENHNLADTVLKITDFGLAREWHKTTKMSAAGTYAWMAPEVIRLSLF
SKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFARLLEECWDPDPHGRPDF
GSILKQLEVIEQSALFQMPLESFHSLQEDWKLEIQHMFDDLRTKEKELRSREEELLRAAQEQRFQEEQLR
RREQELAEREMDIVERELHLLMSQLSQEKPRVRKRKGNFKRSRLLKLREGSSHISLPSGFEHKITVQASP
TLDKRKGSDGASPPASPSIIPRLRAIRLTPMDCGGSSGSGTWSRSGPPKKEELVGGKKKGRTWGPSSTLQ
KERAGGEERLKALGEGSKQWSSSAPNLGKSPKHTPMAPGFASLNEMEEFAEADEGNNVPPSPYSTPSYLK
VPLPAEPSPCVQAPWEPPAVTPSRPGHGARRRCDLALLSCATLLSAVGLGADVAEARAGDGEEQRRWLDS
LFFPRPGRFPRGLSPTGRPGGRREDTAPGLGLAPSATLVSLSSVSDCNSTRSLLRSDSDEAAPAAPSPPP
SPLAPSPSTNPLVDVELESFKKDPRQSLTPTHVTAAHAVSRGHRRTPSDGALRQREPLELTNHGQGPRDP
LDFPRLPDPQALFPTRRRPLEFPGRPTTLTFAPRPRPAASRPRLDPWKLVSFGRTLSISPPSRPDTPESP
GPPSVQPTLLDMDMEGQSQDNTVPLCGVYGSH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081292
ORF Size 2826 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081292.1, NP_001074761.1
RefSeq Size 3690 bp
RefSeq ORF 2829 bp
Locus ID 269881
Cytogenetics 7 A3
Summary Activates the JUN N-terminal pathway.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081292" in other vectors (4)
SKU Description Size Price
MC222759 Map3k10 (untagged) - Mouse mitogen-activated protein kinase kinase kinase 10 (Map3k10), (10ug) 10 ug
$950.00
MR215590 Map3k10 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 10 (Map3k10) 10 ug
$589.00 $862.00
MR215590L3 Lenti ORF clone of Map3k10 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 10 (Map3k10) 10 ug
$1,162.00
MR215590L4 Lenti ORF clone of Map3k10 (mGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase 10 (Map3k10) 10 ug
$1,162.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.