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Tomt (NM_001081679) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215579
Tomt (tGFP-tagged) - Mouse transmembrane O-methyltransferase (Tomt), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Tomt or NM_001081679"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tomt
Synonyms Comt2; F930017I19Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215579 representing NM_001081679
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCCTGCCATTGCACTGGCATTCCTGCCACTCGTGGTAACACTGCTGGTGAGATACCGGCACCATT
TCCGACTGCTGGTGCGTACAGTCCTGCTGAGAGGATTTCGAGACTGCCTGTCAGGACTTCGGATTGAGGA
GCGGGCTTTCAGCTATGTGCTCACCCATGCCCTGCCTGGAGACCCTGGTCACATCCTCACCACGCTTGAC
CATTGGAGCAGCTGCTGCGAGTACCTGAGCCACATGGGCCCTGTTAAAGGTCAGATTCTGATGCGGCTGG
TGGAAGAGAAGGCCCCTGCTTGTGTGCTGGAGCTGGGCACATACTGTGGATACTCTACACTGCTTATTGC
GCGAGCCTTGCCCCCTGGAAGTCGCCTTCTCACTGTGGAGCGGGACTCACGTACAGCAGCAGTGGCTGAA
AAAGTCATCCGGCTGGCAGGCTTCGATGAGCAAATGGTGGAGCTCATTGCAGGCAGCTCAGAGGAAGTGA
TCCCACGCCTAAGAGCTCAGCACCAGCTGAATCGTGCAGATCTGGTGCTCCTGGCACACCGACCTCGGTA
TTACTTAAGGGATCTGCAGCTACTGGAGGCCCATGCTCTGCTCCCACATGGCGCCACTGTATTGGCTGAC
CATGTGCTTTTTCCTGGTGCACCCCGCTTCCTACAGTACACCAAGAGCTGTGGTCGCTATCGCTGCCGCC
TTCACCACACCAGCCTCCCAGACTTCCCTGCCATTAAAGATGGCATAGCCCAACTCACCTATACTGGACC
CGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215579 representing NM_001081679
Red=Cloning site Green=Tags(s)

MSPAIALAFLPLVVTLLVRYRHHFRLLVRTVLLRGFRDCLSGLRIEERAFSYVLTHALPGDPGHILTTLD
HWSSCCEYLSHMGPVKGQILMRLVEEKAPACVLELGTYCGYSTLLIARALPPGSRLLTVERDSRTAAVAE
KVIRLAGFDEQMVELIAGSSEEVIPRLRAQHQLNRADLVLLAHRPRYYLRDLQLLEAHALLPHGATVLAD
HVLFPGAPRFLQYTKSCGRYRCRLHHTSLPDFPAIKDGIAQLTYTGPG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081679
ORF Size 774 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081679.1, NP_001075148.1
RefSeq Size 1354 bp
RefSeq ORF 777 bp
Locus ID 791260
UniProt ID A1Y9I9
Cytogenetics 7
Summary Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones (PubMed:18794526). Required for auditory function (PubMed:18794526, PubMed:28504928). Component of the cochlear hair cell's mechanotransduction (MET) machinery. Involved in the assembly of the asymmetric tip-link MET complex. Required for transportation of TMC1 and TMC2 proteins into the mechanically sensitive stereocilia of the hair cells. The function in MET is independent of the enzymatic activity (PubMed:28504928).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081679" in other vectors (4)
SKU Description Size Price
MC215454 Tomt (untagged) - Mouse transmembrane O-methyltransferase (Tomt), (10ug) 10 ug
$330.00
MR215579 Tomt (Myc-DDK-tagged) - Mouse transmembrane O-methyltransferase (Tomt) 10 ug
$289.00 $300.00
MR215579L3 Lenti ORF clone of Tomt (Myc-DDK-tagged) - Mouse transmembrane O-methyltransferase (Tomt) 10 ug
$600.00
MR215579L4 Lenti ORF clone of Tomt (mGFP-tagged) - Mouse transmembrane O-methyltransferase (Tomt) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.