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AY358078 (NM_194347) Mouse Tagged ORF Clone

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SKU
MG215506
AY358078 (tGFP-tagged) - Mouse cDNA sequence AY358078 (AY358078), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol AY358078
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215506 representing NM_194347
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCAAGAGGAAAGCCAAGCAGAAGGCATCCATTTCCAGCACCCTCAACACATTTTCACAGGACTGG
GTACCTCAGGTCTTCAGTCCTGTACCAGGAGAGAACTAGAATCTTCTGTGATGTCACCTGTGTTTTGGTT
ACAACAACTGCTTGTGGGATATTCATTCAGTGCCTTTTGGGTTCTCAATCAGGCATTTTTTCCCGGCTGC
TCAGGCTATTTCACAAGGAGAATGGCAATCAAGGAGAGACCAGACCAAGTGTCTGCTGGGAAAACATCAT
CCCAAAATTGCAACATCACTAATCATATGAAGAATGTGAATAAAGTAGAAGATATGAAACTTTATATCAG
GAAGATCAATGCTGAGAGGGAAGAACTTTGTCGAATCTTGGACACTGACATGAATACTGATTTGAACTAC
AGGATGAACATTGAATTCACCATCATTAAATCACAAGATGAGAAGACAATGTCGGATATGGAAAAAATGA
CCCAGTCAATAAGTGATACCATTGAGAAGTACAAGGAATTCATAGAAGATAATGATTCCTACAGCATCAG
GCATACCCACCTCCTGAAAGAATGCAATCAATTGAAGGAAAAAGTAAGGATGCTGCTGAATGAGAACAGA
AAGCTGCTGGTAGAGCAGGCTGACCAGGAAGCATCCTATGGTGAAGAAAAGAGGTTCTGTGATGAGACCA
GCAAGAACATACATCCCACATCAAAGCAGCAGCAGGTGTATCAGAGGCAGATTGCCCTCATGGCTAACCA
TACATATAGACAATCCAATGTAACAGTCCACCAACTTATCCAGGCACTCATCTGTGTCTCCTTCAAGTGT
TCATTTAGGTTGAAGGTGGTAGCCAAAGACAGCACAATCAATGTGGAAAGCCTTTGCATGATTTCTCAAC
TCTTACACCTGCAAGTTGTGTATGAGGGAGATCTTGACCTGGTCTGTGCCCTGTTTGAGCTTCTGCCGAA
TATTTCAGACATAGGGGAGGAAGAGACAAAGGCCTGGAGGCAAAGAAAGTGGGGGATCTTCGATGAATCA
ACTCCTGTGGAAACTCGTCAACGTTTGAATCCACCCGGTGGGAAGACCAGTGACATATTTGGGTCCCCAG
TCACTGCCACTGTGCCTCTGGCACACCCAAACAAGCCCAAGGATCATGTTTTGCTGTGTGAAGGTGAAGA
CTCTAAGTCTGACCTGAAATCTGCAACAGACTCCACACCCAGAGGAGAGCAGGGCGACAAAGGAAGCTCA
AAAGAAGTAGAGCATGCAAAGATACCGGAGCCCACAGCTACAGTTGACAGTCATGAGCCCAGACTGGGGC
CACGACCTCGCTCCCACAACAAAGTCCTGAACCCACCAGGAGGCAAATCCAGCCTCTCCTTCTAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215506 representing NM_194347
Red=Cloning site Green=Tags(s)

MEQEESQAEGIHFQHPQHIFTGLGTSGLQSCTRRELESSVMSPVFWLQQLLVGYSFSAFWVLNQAFFPGC
SGYFTRRMAIKERPDQVSAGKTSSQNCNITNHMKNVNKVEDMKLYIRKINAEREELCRILDTDMNTDLNY
RMNIEFTIIKSQDEKTMSDMEKMTQSISDTIEKYKEFIEDNDSYSIRHTHLLKECNQLKEKVRMLLNENR
KLLVEQADQEASYGEEKRFCDETSKNIHPTSKQQQVYQRQIALMANHTYRQSNVTVHQLIQALICVSFKC
SFRLKVVAKDSTINVESLCMISQLLHLQVVYEGDLDLVCALFELLPNISDIGEEETKAWRQRKWGIFDES
TPVETRQRLNPPGGKTSDIFGSPVTATVPLAHPNKPKDHVLLCEGEDSKSDLKSATDSTPRGEQGDKGSS
KEVEHAKIPEPTATVDSHEPRLGPRPRSHNKVLNPPGGKSSLSFY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_194347
ORF Size 1395 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_194347.1, NP_919328.1
RefSeq Size 1461 bp
RefSeq ORF 1398 bp
Locus ID 278676
Cytogenetics 14 C1- C2
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Documents
Resources
"NM_194347" in other vectors (4)
SKU Description Size Price
MC216384 AY358078 (untagged) - Mouse cDNA sequence AY358078 (AY358078), (10ug) 10 ug
$503.00
MR215506 AY358078 (Myc-DDK-tagged) - Mouse cDNA sequence AY358078 (AY358078) 10 ug
$289.00 $457.00
MR215506L3 Lenti ORF clone of AY358078 (Myc-DDK-tagged) - Mouse cDNA sequence AY358078 (AY358078) 10 ug
$757.00
MR215506L4 Lenti ORF clone of AY358078 (mGFP-tagged) - Mouse cDNA sequence AY358078 (AY358078) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.