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Atpaf1 (NM_181040) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215483
Atpaf1 (tGFP-tagged) - Mouse ATP synthase mitochondrial F1 complex assembly factor 1 (Atpaf1) nuclear gene encoding mitochondrial protein, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atpaf1
Synonyms 6330547J17Rik; AI593252; ATP11; ATP11p
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215483 representing NM_181040
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCAGGCGCGCGGGCCGGGGGCCGAGGCTGACGCGCCCGAGGAGGAGGAGGGGGAGTCGCAGGCGG
CCATGGCGGCGGTAGTGTCCGCTGCGGGTGGAGCGTGCCCCGCAGTCCTGCAGGTGGCGGGTCTCTACCG
AGGCCTGTGTGCAGTGCGCAGCCGCGCCCTGGGGCTGGGGTTCGTGTCACCGGCACAGTTGCGCGTGTTC
CCGGTGCGCCGGGGCTCGGGCCTGCCCCCCGAGGGAGCAGACGGCAGTGGGGTCAGCGAGCTGGAAGCGA
ACCCCTTCTACGACCGCTACCGGGACAAGATCCAGCAGCTGCGCAGGTCTGACCCAGCGGCCTTTGAGTC
CCGCCTGGAGAAGCGCAGTGAGTTCCGAAAGCAGCCAGTGGGGCACTCCAAACAAAGTGATTTTATCAAG
TGTATGGAGCAGAAGACAGATGCCTTGGGGAAACAGCCTGTGAGCAAAGGATTCACTAAGGACAAGACTC
TCAGTTCAGTCTTCAACGTTGAGATGGTGAAAGACAAAACTGCGGAGGAAATAAAACAGATTTGGCAGCA
GTATTTTTCAGCAAAAGACACAGTCTACGCAGTTATTCCTAAAGAGAAGTTTGATTTGATCTGGAACAGG
GCTCAGTCCTGCCCAACATTTCTTTGTGCACTACCAAGAAGGGATGGTTATGAGTTCTTTGTAGGACAAT
GGACAGGTACAGAGCTCCACTTCACTGCACTTATAAATATTCAGACCCGAGGGGATGCAGCAGCCAGCCA
GCTGATTTTATATCACTATCCTGAACTTAAGGAAGAAAAGGGCATAGTGCTGATGACAGCAGAAATGGAC
TCCACATTTCTGAATGTTGTGGAGGCACAGTGCATCGCCAATCAGGTTCAGCTCTTCTACGCCACCGATC
GGAAGGAGATCTATGGGCTAGTAGAGACCTTTAACTTCAGACCAAATGAGTTCAAATATATGTCTGTCAT
CGCTGAGTTGGAGCAAAGTGGACTTGGAGCCGAACTGAAACGTGCCCAGAACCAAGATAAGACT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215483 representing NM_181040
Red=Cloning site Green=Tags(s)

MEQARGPGAEADAPEEEEGESQAAMAAVVSAAGGACPAVLQVAGLYRGLCAVRSRALGLGFVSPAQLRVF
PVRRGSGLPPEGADGSGVSELEANPFYDRYRDKIQQLRRSDPAAFESRLEKRSEFRKQPVGHSKQSDFIK
CMEQKTDALGKQPVSKGFTKDKTLSSVFNVEMVKDKTAEEIKQIWQQYFSAKDTVYAVIPKEKFDLIWNR
AQSCPTFLCALPRRDGYEFFVGQWTGTELHFTALINIQTRGDAAASQLILYHYPELKEEKGIVLMTAEMD
STFLNVVEAQCIANQVQLFYATDRKEIYGLVETFNFRPNEFKYMSVIAELEQSGLGAELKRAQNQDKT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181040
ORF Size 1044 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181040.5
RefSeq Size 2330 bp
RefSeq ORF 1047 bp
Locus ID 230649
Cytogenetics 4 D1
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Documents
Resources
"NM_181040" in other vectors (4)
SKU Description Size Price
MC212888 Atpaf1 (untagged) - Mouse ATP synthase mitochondrial F1 complex assembly factor 1 (Atpaf1), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$503.00
MR215483 Atpaf1 (Myc-DDK-tagged) - Mouse ATP synthase mitochondrial F1 complex assembly factor 1 (Atpaf1), nuclear gene encoding mitochondrial protein 10 ug
$289.00 $457.00
MR215483L3 Lenti ORF clone of Atpaf1 (Myc-DDK-tagged) - Mouse ATP synthase mitochondrial F1 complex assembly factor 1 (Atpaf1), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR215483L4 Lenti ORF clone of Atpaf1 (mGFP-tagged) - Mouse ATP synthase mitochondrial F1 complex assembly factor 1 (Atpaf1), nuclear gene encoding mitochondrial protein 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.