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Slc35g1 (NM_175507) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215476
Slc35g1 (tGFP-tagged) - Mouse transmembrane protein 20 (Tmem20), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc35g1
Synonyms AA590464; D330039I19Rik; Tmem20
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215476 representing NM_175507
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCCCCCGGAGAGCGCGGCGGAGCTTGCAGCCGAGGCGGTGGAGCTGCGCGAGCCCGAGCTGCAGC
TCGCGGACCCGGCGTCCCCCGGCGAGGAGCACGTGGACGTCGAGGCGGAGGGCGCCCCTGGCCGCGGCAG
GTGCTGGCCGTGTGGAGCCTGGGCGTGCGGCTCGCGTGGGGAGCCCGAAGCCAAGAAGAAAGCACCCTGC
CCCGGACTTGGCTTGTTTTACACGGTGTTGTCTGCCTTCCTTTTCTCAGTGGCCTCTTTATTTGTTAAAA
AAGTGCAAGGCGTCCATGCTGTAGAGATCAGTGCGTTTCGATGCGTGGTCCAAATGCTAGTTATTATCCC
TTGCTTAATATACAGAAAAACGGGGTTCATAGGTCCCAAAGGCCAGCGACTCTTCCTCTTCCTCAGAGGG
GTCTTTGGTTCCAGTGCCATGATCCTCATGTATTATGCGTTCCAGACAACGTCCCTCGCAGACGCCACAG
TTATTGCGTTTAGCTGTCCCGTGTTTACGTCCATATTTGCTTGGATATTTCTCAAGGAAAAGTATAGCCT
CTGGGATGCTTTCTTCACCTTGTTTGCAATCGCTGGAGTGATACTTATCGTGAGACCACCATTTATATTT
GGTTCCGACACTTCAGGGATGAGAGAAAGCTATTCAGAGCACATTAAGGGGACGTTTGCTGCGATCGGAC
ACGCTGTGCTAGCTGCGATCACTCTGGTTATTCTGAGGAAAATGGGAAAATCTGTGGACTACTTTCTGAG
CATCTGGTACTATGTCATACTTGGCCTTCCCGAAGCCATCATCATCCTCTTTGTCATAGGAGAGTGGAGT
CTGCCCTACTGTGGGCTGGACAGGCTGTTTCTCATACTCATTGGACTCCTAGGTTTGGGGGGTCAGATAT
TTATCACTAAAGCAGTTCAAATAGAAAAAGCAGGACTCGTAGCGATAATGAAGACCATGGATATAGTCTT
TGCTTTCATCTTTCAGATTGCTTTCTTTGATAATGTACCAACTTGGTGGACGGTGGGTGGGGCCCTCTGT
GTGGTGGTCAGCACCACTGGAGCAACCATTCGTCGATGGCTGCAGGGTTCCAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215476 representing NM_175507
Red=Cloning site Green=Tags(s)

MGPPESAAELAAEAVELREPELQLADPASPGEEHVDVEAEGAPGRGRCWPCGAWACGSRGEPEAKKKAPC
PGLGLFYTVLSAFLFSVASLFVKKVQGVHAVEISAFRCVVQMLVIIPCLIYRKTGFIGPKGQRLFLFLRG
VFGSSAMILMYYAFQTTSLADATVIAFSCPVFTSIFAWIFLKEKYSLWDAFFTLFAIAGVILIVRPPFIF
GSDTSGMRESYSEHIKGTFAAIGHAVLAAITLVILRKMGKSVDYFLSIWYYVILGLPEAIIILFVIGEWS
LPYCGLDRLFLILIGLLGLGGQIFITKAVQIEKAGLVAIMKTMDIVFAFIFQIAFFDNVPTWWTVGGALC
VVVSTTGATIRRWLQGSK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175507
ORF Size 1104 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175507.3, NP_780716.1
RefSeq Size 3471 bp
RefSeq ORF 1107 bp
Locus ID 240660
UniProt ID Q8BY79
Cytogenetics 19 C3
Summary May play a role in intracellular calcium sensing and homeostasis. May act as a negative regulator of plasma membrane calcium-transporting ATPases preventing calcium efflux from the cell (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175507" in other vectors (4)
SKU Description Size Price
MC213104 Slc35g1 (untagged) - Mouse transmembrane protein 20 (Tmem20), (10ug) 10 ug
$503.00
MR215476 Slc35g1 (Myc-DDK-tagged) - Mouse transmembrane protein 20 (Tmem20) 10 ug
$289.00 $457.00
MR215476L3 Lenti ORF clone of Slc35g1 (Myc-DDK-tagged) - Mouse transmembrane protein 20 (Tmem20) 10 ug
$757.00
MR215476L4 Lenti ORF clone of Slc35g1 (mGFP-tagged) - Mouse transmembrane protein 20 (Tmem20) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.