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Ddias (NM_001080995) Mouse Tagged ORF Clone

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SKU
MG215432
4632434I11Rik (tGFP-tagged) - Mouse RIKEN cDNA 4632434I11 gene (4632434I11Rik), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,023.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ddias
Synonyms 4632434I11Rik; noxin
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215432 representing NM_001080995
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATAAAAGACGGAAATTCCTTTTTGCCTCAGTACTTGCTGTCCAGAATTGGAATTTTATATATCCAT
CATGTCAAAGGTGCTTCTCTAAGATAATCCTTGAGTCCAAAAGGTTTACTTGTCCCAAATGTGGCTCTTC
TGGTGACACTGGAAGTACCAATTACAGATACAAACTGTCCTTGAAAGTGGCAGAATCCAGCAAACTATTT
TTCATTACTGTATTTGGGAGCTGCTTAGATACATTTTTTGGACTTACGGCCACTGGTTTACACAGGTACC
TTGAAGATTCTATTAAAAACCTAGAAACCCTGGACAGTGGTAGAACTCAGAGCCTACTGACCACAGCAGT
AGAAAAGTGTTTTGTTGGACAAAGCTTTGTATTTGGAGTAACGAATTTTGGAGATGTATGTGGACATGAT
TCAGATTCCAGTAACTTTCAGCAGCCTTGCTGTAAGCTCAGAGGAGAAGTCAGGACACTTGTAGCTTCCC
AGATTATTCTGCCTAGCCCACATGTTAAAGGATTTACAGTCATTGCCTATTTGCATCCGCTTTTACACAA
GAAACCTCACTGTGGCTCCCAGGAACATAGCAGCCAGTCACTCACTTCAGATGACTCAGACAGTGATCTT
AACAACATACAGGGCTCTGGGAATACATCCTGGTTTTCAGAGTCTAGTGTCGGAGAGGATTTTTTCAGAT
TCTGGGAGCCATCTCTTGAACTGACTTCTTCTGACTCACAAGTAACATCTAGTGATGACTTTCCAGCATC
AAAACAAATCATGGCCAGTGGTACTCCTAACCAAAACAGACACTGCATCTCCGTTTCCGAGGTCACTAGT
TCTAAGAACTGCCATGATTCCCTTCAGAGTTTATGGAGCCTTCATTCTTGTATGGACAAGAATAATACAA
CAGGAAAGTTGGGTGAAGAATTAGGCTTACCGAGTCCAGTTTGCAACAGTTGCCATGAAAGTAGATTGTC
TGACTCTCATTTTTTCCCTTCACAAATGCAAGAACCCTTCGAGGAACATAATTTAGAATGCTATAGTAAA
GCAGGAAAAGATGATTATTCCCAGTATGATATCGCATGTTACCAGCATTATGAAGTAAATACTACCCCCA
TCCTTCAACAGAGGTCTTCTGCATTTTCATTGTCATCACTGAAACCCGAAGAAACAGCGAATCCTTCCCA
GAACTCTGACTCCTTGATTTGGGATGATCTACCACTCTCTGAAAGTCTGAACAAGTTTCTAGCAGTTGTT
GAAAGTGAGGTTGCTGTAACTGAGATAGATGCTAAGAACAGAAAACAGGGTATGGATAAAAGCATTGATA
AATGTCACAAAAACTACAGCAGATTATCACTGACTCCATGGAAAAATACTAGACCCTTAAATACATCATC
TTTCAGCTTAAGATCATCACAAGCAATGATGAAAGAAGACTCTGGGAAAGAGGCCCTCTTTTCCAACTGT
AAGTCTAATGCAAGTCCTCACATTCAGAAAGAATCGAAAGCAGACAAGACAGAAGCGGCTGTCTCCATAG
CCGGTAGTAGAAGTGATATCTCTGAGGATTTTCTACCAGATACTTGCTCATCAGCACTGTTTACATCTTC
AAAAGATAGGGAAGCCTTCATTACCCAAAAGAGGACTAGTGGGGTCTTGCAACAAAAGAATGAAATTTCA
TGCAGGCCCAGTACCTCAATAAGTGACTGTTCTGATCTCAGGAGCAGATATTTTACTGGATGTGGACAAA
AATTACATTCAGGAACAAAAGGAAAATTGGCAATACAAAACTGTTCTAAAAAATACAGCGATGCTTCTGA
TCTTCACAAATTAGAAAATAAGCATAGATGGCCAAAAAAGGAAGATGACAATTTCACAATATGCAGAAAA
CTCACATATCCTTTAGAGACCCTTTGCAGTAGCCCAGAAAGTATAAATACATCGAAAGAGATGCCTTGCA
GGCCTAGCAATAATAACTTAACACCAAGTTCTTCTGGTGATCATGAAGGTAGTTACAATGCTTCTGCTGA
TCTTTTTGATTATATTGCTAACGATAAGGACATTGGAACAGAAATTACCAAAATACCACAGGATAACTTG
TTACCACTGGAAGTACCTTGCACAGAAAATTATCCTATAAATGAGAACCGTGGCCAGCCTTCACAAAAAT
TATCCTTGCAAAGCATATCACCCTCAAGGTATTCAAGACCAAGGTCTCAGTCAGACTCAGAATGTGATTT
TGAAGAAAGCCAAGACTTTGTTCCATGTTCACAGTCAACTCCAGTTGCAGGCTTCCACCAAAGAATTCAT
GGCTTAAATGGTGCTTCCAAAATACTGCCTTCCATATATTCAAATCCTAATGCTAATTATAAAAACAGAA
AAAATTCTCCTCTAACTGGCCAGTATGAAGCCACCTCAGCCTGCTCAAAGAATGTAAAAGCATTTCGGCA
GAAACCCGAAAGTCCTTTTGTGTCTAGTTTGACACAAGCAAATGTTTTCAACCACTGCCCTGCTGCTGAG
AGCCTTGAAAATGATGTGGATGAATGGGTCCCCCCTACCACCAAGAAAGTATTTATTTCAGATATTCTTG
GATTCAGAGTCATGGGTTTAAGAAAATGCCTTGATCTCCATTATTCCCCTGACCCAAAAGAGTTACCAAG
GAAGAAACTGATAAAAGTCACACATAAAACCAACATT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215432 representing NM_001080995
Red=Cloning site Green=Tags(s)

MNKRRKFLFASVLAVQNWNFIYPSCQRCFSKIILESKRFTCPKCGSSGDTGSTNYRYKLSLKVAESSKLF
FITVFGSCLDTFFGLTATGLHRYLEDSIKNLETLDSGRTQSLLTTAVEKCFVGQSFVFGVTNFGDVCGHD
SDSSNFQQPCCKLRGEVRTLVASQIILPSPHVKGFTVIAYLHPLLHKKPHCGSQEHSSQSLTSDDSDSDL
NNIQGSGNTSWFSESSVGEDFFRFWEPSLELTSSDSQVTSSDDFPASKQIMASGTPNQNRHCISVSEVTS
SKNCHDSLQSLWSLHSCMDKNNTTGKLGEELGLPSPVCNSCHESRLSDSHFFPSQMQEPFEEHNLECYSK
AGKDDYSQYDIACYQHYEVNTTPILQQRSSAFSLSSLKPEETANPSQNSDSLIWDDLPLSESLNKFLAVV
ESEVAVTEIDAKNRKQGMDKSIDKCHKNYSRLSLTPWKNTRPLNTSSFSLRSSQAMMKEDSGKEALFSNC
KSNASPHIQKESKADKTEAAVSIAGSRSDISEDFLPDTCSSALFTSSKDREAFITQKRTSGVLQQKNEIS
CRPSTSISDCSDLRSRYFTGCGQKLHSGTKGKLAIQNCSKKYSDASDLHKLENKHRWPKKEDDNFTICRK
LTYPLETLCSSPESINTSKEMPCRPSNNNLTPSSSGDHEGSYNASADLFDYIANDKDIGTEITKIPQDNL
LPLEVPCTENYPINENRGQPSQKLSLQSISPSRYSRPRSQSDSECDFEESQDFVPCSQSTPVAGFHQRIH
GLNGASKILPSIYSNPNANYKNRKNSPLTGQYEATSACSKNVKAFRQKPESPFVSSLTQANVFNHCPAAE
SLENDVDEWVPPTTKKVFISDILGFRVMGLRKCLDLHYSPDPKELPRKKLIKVTHKTNI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001080995
ORF Size 2697 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001080995.2
RefSeq Size 3450 bp
RefSeq ORF 2700 bp
Locus ID 74041
Cytogenetics 7 E1
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Documents
Resources
"NM_001080995" in other vectors (4)
SKU Description Size Price
MC222472 4632434I11Rik (untagged) - Mouse RIKEN cDNA 4632434I11 gene (4632434I11Rik), (10ug) 10 ug
$906.00
MR215432 4632434I11Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4632434I11 gene (4632434I11Rik) 10 ug
$589.00 $823.00
MR215432L3 Lenti ORF clone of 4632434I11Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4632434I11 gene (4632434I11Rik) 10 ug
$1,123.00
MR215432L4 Lenti ORF clone of 4632434I11Rik (mGFP-tagged) - Mouse RIKEN cDNA 4632434I11 gene (4632434I11Rik) 10 ug
$1,123.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.