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Pik3c2b (NM_001099276) Mouse Tagged ORF Clone

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SKU
MG215420
Pik3c2b (tGFP-tagged) - Mouse phosphoinositide-3-kinase class 2 beta polypeptide (Pik3c2b), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,596.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Pik3c2b or NM_001099276"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pik3c2b
Synonyms C330011J12Rik; Gm199; PI3K-C2beta; PI3KC2beta
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215420 representing NM_001099276
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTCCACTCAGGGCAATGGGGAGCACTGGAAGTCCCTGGAGTCGGTGGGCATCAGCCGCAAAGAGC
TGGCTATGGCCGAAGCCCTGCAGATGGAGTATGACGCTCTTTCCCGGCTCCGACATCACAAGGAGGAGAG
CAGGGCCAAGCAGAACACAGAACCCTCTCTTATCAGCTGGGATGAGCCAGCCTTGGACTTCTACAGCAAG
CCAGCAGGCAGGCGGACCGACCTCAAGCTCTTGCGTGGTCTTTCTGGCTCTGATCCCACCCTAAATTACA
ACTCCATCTCCCCACCAGAGGGGCTCCCCAACTCTACCTCCCAGGATCCACAGCCTGGCCCAGACCCCTG
GCCCAAGGGCTCCCTGTCTGGAGACTATCTCTATATTTTTGATGGCTCAGAGGGGAGATGCTCTTTGTCT
CCAGGATCAGGTGACACAGATGGCTCTTGCAAGAAACTATCCCCACCTCCTCTCCCTCCTCGTGTCTCTA
TCTGGGATGCCCCTCCCTTGCCTCCTAGAAAAGGATCACCCTCACCCTCCAAGATCTCTCAGCCTAATGA
CATCAACAGTTTCTCTTCGGCAGAACAGCCACCTGACAAACTGCTAGTGGCCCAGGACCCAGAAGAGGGA
GAGCTGCCTGATGGCAGGGGACAGGGACACACGCTGGGGTCTGTGGACTACGATGGCATCAATGATGCAA
TTACCAGGCTCAACTTGAAGTCCACCTACGACTCAGAGATATCGTCTGATGCTACCAGGGGCTGGAAGGA
GGGCCGAGGGCCCCTGGACTTCAACAAGGACACCTCTGGGAAACCTGTGGCCCGGAGCAAGACGATGCCC
CCTCAGGTGCCCCCTCGAACCTATACTTCTCGATATGCCAACCGAAAAAATGCAACACCTGGCAATAACC
GACGGATTTCTGCTGCTCCGGTGGGCTCTCGGCCCCACACTGTTGCCAATGGCCACGAATTGTTTGAGGT
CTCAGAGGAGAGAGATGAGGAAGTTGCTGCGTTCTGCCACATGCTGGATATCCTTCGAACGGGCTCTGAT
ATCCAGGACTATTCCCTAACTGGGTGTGTCTGGAGCACTGTCACCCCAAGTCCAGAACACCTTGGGGATG
AGGTCAACCTGAAGGTGACAGTGTTGTGTGACAGTCTCCGGGAGCCACTCACCTTTACCTGCAACTGTTC
CTCCACCGTGGACCTGCTCATCCACCAGACCCTCTGCTACACTCACGATGAGCTGAGGGAAGTGGACGTG
GGCGACTTTGTGCTGAAGCCCTGTGGGCTGGAGGAGTTCTTGCAAAATAAGCACGCCCTGGGAAGCCATG
AGTATATCCAATATTGCCGCAAGTTTGATATCAGCATCCGGCTACAGCTGATGGAGCAGAAGGCTATCCG
GAGCGACCTGGCCCGGACGGTGAATGACGACCAGAGCCCTTCCACCTTGAACTACCTCATCCATCTCCAA
GAGAGGCCAGTCAAGCAGACCATCAGCAGGCAGGCCTTGAGTCTTCTGTTTGACACTTACCACAATGAGG
TGGACGCCTTCCTGCTGGCTGATGGGGATTTCCCACTGAAGGCTGACAGGGTGGTCCAGTCTGTCAAGGC
CATTTGCAATGCTCTGGCTGCTGTGGAGACTCCCGAGATCACGAGCGCTCTCAACCAGCTGCCTCCCTGC
CCCTCGCGCATGCAGCCAAAAATCCAGAAGGATCCCAGCGTGTTGAGTGTGAGGGAAAACCGAGAGAAGG
TGGTAGAAGCCTTGACAGCGGCCATCTTGGACTTGGTGGAGCTGTACTGCAGCACATTCAATGCAGACTT
CCAGACGGCGGTGCCTGGGAGCCGAAAGCATGATTTGGTTCAAGAGGCCTGCCACTTCCCTGGGGCCCTG
GCCTTCACTGTCTATGGCACTCACCGTATTCCCATCATCTGGGCTACCAGCTATGAAGATTTCTACCTCT
CCTGCTCCCTCAGCCACGGTGGCAAGGAACTTTGCAGCCCCCTGCAGACCCGGCGGGCTCACTTCTCCAA
GTACCTGTTCCACCTCATCATCTGGGACCAGCAGATCTGCTTTCCAGTGCAGGTGAACAGGCTACCTCGG
GAGACTCTGCTGTGTGCCACACTCTATGCCCTGCCCGTTCCCCCTCCTGGGGGTTCCTCTGAAGCGAATA
AGCAGAAGCGGGTGCCTGAAGCTCTGGGTTGGGTCACTACCCCTCTCTTCAACTTCAGGCAAGTCTTGAC
CTGTGGTCGAAAGCTCCTGGGCTTGTGGCCAGCGACACAGGAAAACTCCGGTGCCCGCTGGAGTGCGCCT
AATTTCCACCAGCCAGACAGTGTTATCCTGCAGATTGACTTCCCCAGCTCGGCCTTTGACATCAAGTTCA
CCAGCCCCCCTGGAGACAAGTTCAGCCCCCGCTATGAGTTTGGCAGTCTCCGGGAGGAGGACCAGCGCAA
ACTTAAAGACATTACGCAGAAGGAGTCCCTATACTGGCTCACGGATGCTGACAAGAAGCAGCTGTGGGAG
AAACGCTATTACTGCCACACTGAGGTGAGCTCACTCCCCCTGGTTCTCGCCAGTGCACCCAGCTGGGAGT
GGGCTTGCCTGCCGGACATCTATGCTCTCCTGCAACAGTGGACTCACATGAACCACCAAGATGCCCTGGG
ACTCCTGCATGCCACCTTCCCGGACCAGGAGGTGCGGCGGATGGCCGTGCAGTGGATCGGCTCCCTCTCA
GATGCGGAGCTGCTGGACTACCTGCCCCAGCTAGTCCAGGCCCTGAAGTATGAGTGCTACCTGGATAGCC
CCTTGGTGCGCTTTCTCCTGAAACGAGCCATCTCTGACCTGCGAGTGACCCACTACTTCTTCTGGTTACT
GAAGGACAGCCTCAAGGACTCTCAGTTCAGTATCCGCTACCAATACCTGCTAGCAGCCCTGCTGTGCTGC
TGTGGCAAGGGACTGAGGGAGGAGTTTAACCGCCAGTGCTGGCTTGTCAACACCCTGGCCAAACTGGCCC
AGCAGGTCCGAGAGGCCACCCCATCCGCCCGCCAGGGCATCCTTCGTGTGGGCTTGGAGGAGGTGAAGCA
GTTCTTTGCCCTCAATGGTTCGTGCCGTCTGCCGCTCAGTCCCAGTCTCCTGGTGAAGGGCATCGTGCCC
AGGGACTGCTCCTACTTCAATTCCAACGCAGTGCCCCTGAAGCTTGCCTTCCAAAATGTGGATCCGCTGG
GCGAGAACATCAGAGTCATCTTTAAGTGTGGGGATGATCTTCGTCAGGACATGCTCACCCTGCAGATGAT
TCGCATCATGAGCAAGATCTGGGTACAGGAGGGGCTGGACATGCGCATGGTCATCTTCCGCTGCTTCTCC
ACTGGCCGAGGAAAAGGGATGGTAGAGATGATACCCAATGCAGAGACACTGCGCAAGATCCAGGTGGAAC
ATGGGGTGACTGGCTCCTTCAAGGACCGTCCCCTGGCCGACTGGCTACAAAAACACAACCCTGGGGAGGA
TGAATATGAAAAGGCTGTGGAGAACTTCATCTACTCTTGTGCTGGCTGCTGTGTAGCTACATACATCTTG
GGCATCTGTGACAGACACAATGACAACATCATGCTAAAGACCACGGGCCACATGTTCCATATAGATTTCG
GCCGCTTCCTGGGTCACGCACAGATGTTTGGTAACATCAAGAGGGACCGTGCCCCGTTTGTCTTCACCTC
AGACATGGCGTATGTCATCAATGGGGGTGACAAGCCTTCCAGCCGCTTCCATGATTTTGTTGACCTTTGC
TGCCAAGCCTACAACCTCATTCGCAAGCACACCCACCTCTTCCTCAACCTTCTGGGCCTGATGCTGTCCT
GTGGAATCCCCGAGCTCTCAGACCTGGAGGACCTCAAGTATGTGTATGATGCCCTGAGACCTCAGGACAC
AGAGGCCAATGCCACCACCTACTTCACAAGGTTGATTGAGTCCAGCCTGGGCAGCGTAGCCACGAAACTC
AACTTTTTCATCCATAACCTGGCTCAGATGAAGTTCACAGGCTCAGATGACCGGCTGACCCTCTCCTTCG
CTCCCCGGACACACACTCTCAAGAGCTCTGGCCGCATCCGAGATGTCTTCCTCTGCCGACATGAGAAGAT
CTTCCACCCCAGCAAGGGCTATGTATATGTGGTGAAGGTGATGCGAGAGAATGCTCACGAGGCGACATAC
ATCCAGAGGACATTCGAGGAGTTCCAGGAACTGCACAATAAGCTGCGCCTGCTCTTCCCTTCTTCCTTCC
TGCCCAGCTTCCCTAGCCGGTTTGTGATTGGCCGCTCCCGGGGAGAGGCAGTGGCTGAGAGGAGGAAGGA
GGAGTTAAATGGCTACATCTGGCACCTGATTCATGCAGCCCCTGAGGTGGCTGAGTGTGATCTGGTGTAT
ACTTTCTTCCACCCCCTGCCCCGAGATGAGAAGGCTTCAGGCCCCAGCCCAGCTCCGAAGTCCTCAGATG
GGACGTGGGCCCGGCCGGTTGGGAAGGTCGGTGGGGAGGTGAAGCTGTCTATCTCCTACAAAAACAACAA
ACTCTTCATCATGGTGATGCATATTCGGGGCTTGCAACCGCTCCAGGATGGCAATGACCCTGACCCCTAT
GTAAAGATTTACCTCCTTCCTGACCCTCAGAAAACCACAAAGAGGAAAACCAAGGTGGCCCGGAAAACCT
GCAACCCTACCTACAATGAGATGCTGGTATATGATGGGATCCCCAAGGGAGACCTGCAGCAGCGGGAGCT
ACAGTTGAGTGTGCTGAGTGAGCAAGGATTTTGGGAGAATCTGCTTCTCGGGGAGGTGCACATCCGCCTT
CGGGAGCTGGACCTGGCCCAGGAGAAGACCGGCTGGTTTGGACTGGGTTCTCGAGGTCATGGAACC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215420 representing NM_001099276
Red=Cloning site Green=Tags(s)

MSSTQGNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHHKEESRAKQNTEPSLISWDEPALDFYSK
PAGRRTDLKLLRGLSGSDPTLNYNSISPPEGLPNSTSQDPQPGPDPWPKGSLSGDYLYIFDGSEGRCSLS
PGSGDTDGSCKKLSPPPLPPRVSIWDAPPLPPRKGSPSPSKISQPNDINSFSSAEQPPDKLLVAQDPEEG
ELPDGRGQGHTLGSVDYDGINDAITRLNLKSTYDSEISSDATRGWKEGRGPLDFNKDTSGKPVARSKTMP
PQVPPRTYTSRYANRKNATPGNNRRISAAPVGSRPHTVANGHELFEVSEERDEEVAAFCHMLDILRTGSD
IQDYSLTGCVWSTVTPSPEHLGDEVNLKVTVLCDSLREPLTFTCNCSSTVDLLIHQTLCYTHDELREVDV
GDFVLKPCGLEEFLQNKHALGSHEYIQYCRKFDISIRLQLMEQKAIRSDLARTVNDDQSPSTLNYLIHLQ
ERPVKQTISRQALSLLFDTYHNEVDAFLLADGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPPC
PSRMQPKIQKDPSVLSVRENREKVVEALTAAILDLVELYCSTFNADFQTAVPGSRKHDLVQEACHFPGAL
AFTVYGTHRIPIIWATSYEDFYLSCSLSHGGKELCSPLQTRRAHFSKYLFHLIIWDQQICFPVQVNRLPR
ETLLCATLYALPVPPPGGSSEANKQKRVPEALGWVTTPLFNFRQVLTCGRKLLGLWPATQENSGARWSAP
NFHQPDSVILQIDFPSSAFDIKFTSPPGDKFSPRYEFGSLREEDQRKLKDITQKESLYWLTDADKKQLWE
KRYYCHTEVSSLPLVLASAPSWEWACLPDIYALLQQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSLS
DAELLDYLPQLVQALKYECYLDSPLVRFLLKRAISDLRVTHYFFWLLKDSLKDSQFSIRYQYLLAALLCC
CGKGLREEFNRQCWLVNTLAKLAQQVREATPSARQGILRVGLEEVKQFFALNGSCRLPLSPSLLVKGIVP
RDCSYFNSNAVPLKLAFQNVDPLGENIRVIFKCGDDLRQDMLTLQMIRIMSKIWVQEGLDMRMVIFRCFS
TGRGKGMVEMIPNAETLRKIQVEHGVTGSFKDRPLADWLQKHNPGEDEYEKAVENFIYSCAGCCVATYIL
GICDRHNDNIMLKTTGHMFHIDFGRFLGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDLC
CQAYNLIRKHTHLFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFTRLIESSLGSVATKL
NFFIHNLAQMKFTGSDDRLTLSFAPRTHTLKSSGRIRDVFLCRHEKIFHPSKGYVYVVKVMRENAHEATY
IQRTFEEFQELHNKLRLLFPSSFLPSFPSRFVIGRSRGEAVAERRKEELNGYIWHLIHAAPEVAECDLVY
TFFHPLPRDEKASGPSPAPKSSDGTWARPVGKVGGEVKLSISYKNNKLFIMVMHIRGLQPLQDGNDPDPY
VKIYLLPDPQKTTKRKTKVARKTCNPTYNEMLVYDGIPKGDLQQRELQLSVLSEQGFWENLLLGEVHIRL
RELDLAQEKTGWFGLGSRGHGT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001099276
ORF Size 4896 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001099276.2, NP_001092746.2
RefSeq Size 7929 bp
RefSeq ORF 4899 bp
Locus ID 240752
Cytogenetics 1 E4
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Documents
Resources
"NM_001099276" in other vectors (4)
SKU Description Size Price
MC224776 Pik3c2b (untagged) - Mouse phosphoinositide-3-kinase, class 2, beta polypeptide (Pik3c2b), (10ug) 10 ug
$1,536.00
MR215420 Pik3c2b (Myc-DDK-tagged) - Mouse phosphoinositide-3-kinase, class 2, beta polypeptide (Pik3c2b) 10 ug
$589.00 $1,396.00
MR215420L3 Lenti ORF clone of Pik3c2b (Myc-DDK-tagged) - Mouse phosphoinositide-3-kinase, class 2, beta polypeptide (Pik3c2b) 10 ug
$1,696.00
MR215420L4 Lenti ORF clone of Pik3c2b (mGFP-tagged) - Mouse phosphoinositide-3-kinase, class 2, beta polypeptide (Pik3c2b) 10 ug
$1,696.00

file-alt Citations

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