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Mydgf (NM_080837) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215394
D17Wsu104e (tGFP-tagged) - Mouse DNA segment Chr 17 Wayne State University 104 expressed (D17Wsu104e), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Mydgf or NM_080837"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mydgf
Synonyms D17Wsu104e; Il25; Ly6elg
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215394 representing NM_080837
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCCCCAGCGGAGGCTTCTGGACTGCGGTGGTCCTGGCGGCCGCAGCGCTGAAATTGGCCGCCG
CTGTGTCCGAGCCCACCACCGTGCCATTTGACGTGAGGCCCGGAGGGGTCGTGCATTCGTTCTCCCAGGA
CGTAGGACCCGGGAACAAGTTTACATGTACATTCACCTACGCTTCCCAAGGAGGGACCAACGAGCAATGG
CAGATGAGCCTGGGGACAAGTGAAGACAGCCAGCACTTTACCTGTACCATCTGGAGGCCCCAGGGGAAAT
CCTACCTCTACTTCACACAGTTCAAGGCTGAGTTGCGAGGTGCTGAGATCGAGTATGCCATGGCCTACTC
CAAAGCCGCATTTGAGAGAGAGAGTGATGTCCCCCTGAAAAGTGAGGAGTTTGAAGTGACCAAGACAGCA
GTGTCTCACAGGCCTGGGGCCTTCAAAGCTGAGCTCTCCAAGCTGGTGATCGTAGCCAAGGCGGCACGCT
CGGAGCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215394 representing NM_080837
Red=Cloning site Green=Tags(s)

MAAPSGGFWTAVVLAAAALKLAAAVSEPTTVPFDVRPGGVVHSFSQDVGPGNKFTCTFTYASQGGTNEQW
QMSLGTSEDSQHFTCTIWRPQGKSYLYFTQFKAELRGAEIEYAMAYSKAAFERESDVPLKSEEFEVTKTA
VSHRPGAFKAELSKLVIVAKAARSEL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080837
ORF Size 498 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080837.2, NP_543027.1
RefSeq Size 913 bp
RefSeq ORF 501 bp
Locus ID 28106
UniProt ID Q9CPT4
Cytogenetics 17 29.2 cM
Summary The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was identified in error as interleukin 25. This activity has not been reproducible, however, and the function of this protein is currently unknown. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_080837" in other vectors (4)
SKU Description Size Price
MC209808 D17Wsu104e (untagged) - Mouse DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e), (10ug) 10 ug
$330.00
MR215394 D17Wsu104e (Myc-DDK-tagged) - Mouse DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e) 10 ug
$289.00 $300.00
MR215394L3 Lenti ORF clone of D17Wsu104e (Myc-DDK-tagged) - Mouse DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e) 10 ug
$600.00
MR215394L4 Lenti ORF clone of D17Wsu104e (mGFP-tagged) - Mouse DNA segment, Chr 17, Wayne State University 104, expressed (D17Wsu104e) 10 ug
$600.00

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