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Rhox8 (NM_001004193) Mouse Tagged ORF Clone

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SKU
MG215389
Rhox8 (tGFP-tagged) - Mouse reproductive homeobox 8 (Rhox8), (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
  $680.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rhox8
Synonyms Tox
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215389 representing NM_001004193, codon optimized.
Due to the complexity of NM_001004193, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCTTCTTCCTGAGGCTCAATCAAGAAGTGAAACCCAGGATTTCTCGGAGCCGGTCTACCGTCAACA
GCATACATGCTATGGAGCCTCAAGAAGTTACCCAGTCATCCCTGCTGAGAGATGACGAAATTAAGGAGAG
CGACGACGCGGCGGCCTGGATAGTTAGCCAGGAGATGAAAGAAAGGGAGGAGAACGCAGGCATACAGGAC
TGCCCTCTGCTGGGAGCCATGGCTGCCGAGGGCGAGGGCGAAAACGAGGAGGAGGGGGAAGAGGGTGAGG
AAGGCGAGGAAGGAGAAGAGGGAGAAGAGGGCGAGGAAGGCGAGGAGGGGGAGGAGGGTGAGGAAGAAGA
GGAGGAAGAAGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGAGGAAGGA
GAAGAAGGCGAGGAAGGCGAAGAGGGCGACGAGGGCGGGGAGGGGGGCGAGGGTGGCGAGGAAGGTGAGG
AAGAGGGAGAGGAGGAAGAAGGCGGCGAGGGCGAAGGCGAAGAAGAGGAGGAAGAGGAGGAGGAGGAAGA
AGAAGAAGAGGAAGAGGAGGAGGAGGAAGAGGAGGAAGAGGAGGCCGCTGCTGCCGCCGCCGCGCGGGAC
GAAACCACCGCTGGATCATCTGCTCCAGTAGACGATCGGTCCCACGATGCCGGCGCCTCTAGCAATGGAG
AGGACCGCGGCCAAGGCGAGGAACTCATTCCTGGTGGGACTAAGGGACTGGAGGCACTTCCTAGCCCAAG
TGGACAGTTGCCACGGAACCGATATAGATTCACAAAGTTCCAATTGCAGGAACTCGAGAGAATCTTCGAA
AGAAACCACTACCCTTCTGCTGCGGCTCGAAGGGAGCTTGCTAGATGGATCGGAGTGACCGAAAGCAGGG
TAGAAAATTGGTTCAAGTCCCGCAGAGCAAAATATAGAAAATGTCTGATG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215389 representing NM_001004193
Red=Cloning site Green=Tags(s)

MLFFLRLNQEVKPRISRSRSTVNSIHAMEPQEVTQSSLLRDDEIKESDDAAAWIVSQEMKEREENAGIQD
CPLLGAMAAEGEGENEEEGEEGEEGEEGEEGEEGEEGEEGEEGEEEEEEEEEEEEEEEEEEEEEEEEEEG
EEGEEGEEGDEGGEGGEGGEEGEEEGEEEEGGEGEGEEEEEEEEEEEEEEEEEEEEEEEEEAAAAAAARD
ETTAGSSAPVDDRSHDAGASSNGEDRGQGEELIPGGTKGLEALPSPSGQLPRNRYRFTKFQLQELERIFE
RNHYPSAAARRELARWIGVTESRVENWFKSRRAKYRKCLM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001004193
ORF Size 960 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001004193.2, NP_001004193.1
RefSeq Size 1202 bp
RefSeq ORF 963 bp
Locus ID 434768
Cytogenetics X A3.3
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Documents
Resources
"NM_001004193" in other vectors (2)
SKU Description Size Price
MC215009 Rhox8 (untagged) - Mouse reproductive homeobox 8 (Rhox8), (10ug) 10 ug
$450.00
MR215389 Rhox8 (Myc-DDK-tagged) - Mouse reproductive homeobox 8 (Rhox8). Note: ORF is codon optimized 10 ug
$480.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.