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Rgs12 (NM_001163512) Mouse Tagged ORF Clone

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SKU
MG215385
Rgs12 (tGFP-tagged) - Mouse regulator of G-protein signaling 12 (Rgs12) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $862.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rgs12
Synonyms 1200016K18Rik; 4632412M04Rik; AI481290; E130309H11
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215385 representing NM_001163512
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCTCTCTTTTTTCTTTCAGTCTGCAACTGTGTCTGATGGTGAGTTGACTGGAGCTGACCTTAAGG
ACTGCATCAGCAACAACAGCCTGAGCAGCAATGCCAGCCTCCCCAGTGTGCAGAGCTGCCGGCGCCTACG
AGAGAGGAGAGTTGCAAGCTGGGCAGTGTCATTTGAGCGTCTCCTTCAGGATCCTGTTGGTGTTCGCTAC
TTCTCTGATTTTCTACGTAAGGAATTCAGTGAAGAGAACATTTTATTCTGGCAGGCCTGTGAGTGCTTCA
GTCATGTTCCTGCACATGACAAAAAAGAGCTCTCTTACAGGGCCCGGGAGATTTTCAGTAAATTCCTGTG
CAGCAAAGCCACCACTCCTGTCAACATTGACAGCCAGGCCCAGCTGGCAGATGACATTCTCAATGCACCC
CACCCTGACATGTTCAAGGAACAACAGCTCCAGATTTTCAACCTGATGAAGTTTGATAGCTACACTCGCT
TTCTGAAATCCCAGCTGTACCAAGAATGCGTCCTGGCAGAGGTGGAGGGACGTACCCTCCCGGACTCTCA
GCAGGTCCCCAGCAGCCCAGCTTCCAAGCACAGCATCAGCTCTGACCACTCCAATGTGTCCACACCAAAA
AAGTTAAGTGGAAAATCAAAATCAGGACGATCCCTGAATGAAGATGTTGGGGAGGAGGATAGTGAGAAAA
AACGCAGAGGGGCCTTTTTCTCTTGGTCAAGGAGCAGGAGCACGGGGCGGTCCCAGAAGAAGAAGGACCA
TGGTGACCATGCCCATGATGCCCCTCATGCCAATGGAGGCCTCTGCCGTCGGGAGTCCCAAGGCTCTGTA
TCTTCTGCGGGGAGCCTAGACCTGTCAGAGGCCTGTAGGACCTCAGCACTCGAGAAAGATAAGGCTGCCA
AACATTGCTGTGTCCACCTCCCAGACGGGACATCCTGTGTGGTTGCTGTCAAGTCCGGGTTCTCCATCAA
AGAGATTCTGTCTGGATTGTGTGAACGGCATGGCATCAATGGGGCTGCTGTGGACCTCTTCCTGGTGGGC
GGAGACAAGCCTCTTGTGCTGCATCAGGACAGCAGCATCCTGGCCACCAGGGACCTACGCTTGGAAAAGC
GGACTTTGTTTCGGCTGGATCTCGTTCCGATTAACCGGTCAGTGGGACTCAAGGCCAAGCCCACCAAGCC
AGTCACGGAAGTGTTGCGGCCCGTGGTGGCCAAATATGGCCTGGACCTGGGTAGCCTGCTGGTGAGACTG
AGCGGGGAGAAGGAGCCCCTGGATCTTGGTGCCCCTATCTCAAGTCTGGATGGACAACGGGTCATCCTAG
AGGAGAGGGATCCTTCTCGAGGGAAAGTGTCCACAGACAAACAGAAAGGTGCTCCTGTCAAACAAAACTC
AGCTGTGAACTCCAGCCCCAGAAACCACTTGGCTATGGGAGAGGAGAGAACACTAGGCAAGTCTAACTCT
ATTAAAATAAGAGGAGAAAATGGAAAAAGTGCTAGGGATCCCCGCCTTTCAAAGAGAGAAGAATCTATTG
CAAAGATTGGGAAAAAAAAATATCAGAAAATTAATTTGGACGAAGCAGAGGAGTTTTTTGAGCTCATTTC
CAAAGCTCAGAGCAACAGAGCCGATGACCAGCGTGGGCTGCTAAGGAAGGAAGACCTGGTGCTGCCCGAG
TTTCTTCGCTTACCTGCTGGTTCCTCAGAACTTGCCCTGTCCAGCCCACCCCCAGTCAAGGGCTATAGCA
AGAGAGCTGTTACAGGCCATGGCCAGGAGGGAGCAGCTCAGACAGAGGAGAGCTACAGCGACAGCCCAGC
TACCAGCCCTGCCTCAGCCCAGAGCCCCTGCTCAGCCTACAGTCCAGGCTCCGCCCACAGCCCCGGCTCC
GCCCACAGCACCCCTGGGCCTCCTGGGACCACCCAACCTGGAGAGAAGCCTACAAAGCCCTCCTGTGTCT
CCATGGTGCAAGAGGGCACTACACAGGCCTGGAGGAGGCTATCACCAGAAATGGAGGCTGGGGGCATCCA
GACTGTGGAGGATGAGCAGGTGGCTGATCTGACCCTGATGGGGGAGGGGGACATCAGCAGCCCCAACAGC
ACGCTGCTACCACCACCCCCCACACCCCAAGACACACCAGGACCTCCAAGACCAGGTACCTCCAGGTTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215385 representing NM_001163512
Red=Cloning site Green=Tags(s)

MSLSFFFQSATVSDGELTGADLKDCISNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLLQDPVGVRY
FSDFLRKEFSEENILFWQACECFSHVPAHDKKELSYRAREIFSKFLCSKATTPVNIDSQAQLADDILNAP
HPDMFKEQQLQIFNLMKFDSYTRFLKSQLYQECVLAEVEGRTLPDSQQVPSSPASKHSISSDHSNVSTPK
KLSGKSKSGRSLNEDVGEEDSEKKRRGAFFSWSRSRSTGRSQKKKDHGDHAHDAPHANGGLCRRESQGSV
SSAGSLDLSEACRTSALEKDKAAKHCCVHLPDGTSCVVAVKSGFSIKEILSGLCERHGINGAAVDLFLVG
GDKPLVLHQDSSILATRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLDLGSLLVRL
SGEKEPLDLGAPISSLDGQRVILEERDPSRGKVSTDKQKGAPVKQNSAVNSSPRNHLAMGEERTLGKSNS
IKIRGENGKSARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSNRADDQRGLLRKEDLVLPE
FLRLPAGSSELALSSPPPVKGYSKRAVTGHGQEGAAQTEESYSDSPATSPASAQSPCSAYSPGSAHSPGS
AHSTPGPPGTTQPGEKPTKPSCVSMVQEGTTQAWRRLSPEMEAGGIQTVEDEQVADLTLMGEGDISSPNS
TLLPPPPTPQDTPGPPRPGTSRF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001163512
ORF Size 2169 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163512.1, NP_001156984.1
RefSeq Size 3434 bp
RefSeq ORF 2172 bp
Locus ID 71729
Cytogenetics 5 17.96 cM
Summary Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001163512" in other vectors (3)
SKU Description Size Price
MR215385 Rgs12 (Myc-DDK-tagged) - Mouse regulator of G-protein signaling 12 (Rgs12), transcript variant 2 10 ug
$589.00 $662.00
MR215385L3 Lenti ORF clone of Rgs12 (Myc-DDK-tagged) - Mouse regulator of G-protein signaling 12 (Rgs12), transcript variant 2 10 ug
$962.00
MR215385L4 Lenti ORF clone of Rgs12 (mGFP-tagged) - Mouse regulator of G-protein signaling 12 (Rgs12), transcript variant 2 10 ug
$962.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.