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Cerkl (NM_001048176) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215371
Cerkl (tGFP-tagged) - Mouse ceramide kinase-like (Cerkl), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $689.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Cerkl or NM_001048176"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cerkl
Synonyms Gm1958; Rp26
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215371 representing NM_001048176
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCGGGGGAGACGCAGGAGCCGGGTGGGCGCCCTGGAGGAGGCGCCTCCGGAGAACCCAAAGGCAG
CTGCTGAACCCGAGCGGGTTTTGCTGCGTGGCATCTTCGAGATCCGGAGGGAAAGCTGCGATGTGGCGTT
GGGCGAGCGGGCCCTGCGGTGGCGACGCATCCAGCCCGAGCTCCCGGCTGGTGGCACCAGGCATGATCTG
CTGAGCAAAGAAGAGTCCCTAGAACTTGAAGACATCTTCTCAGTGAAGCTGAAGCGGCGTTGCTGGGCCA
AGCATCCAGGCAGAGACACACTGCTGGGCATAACGCTTTTCATCTGCTTGAAGGAGCAACGGAACAAACT
AAAGGACTCCACGCTGGATCTTATTAACTTCAGTGAAGACCACTGTGACATATGGTTTAGACAGTTCAAG
AAAATCTTGGATGGTTTCACAAGCAGGCCCAAGGCTCTCAAGATCCTCCTCAACCCCCAGAGTCACAGAA
AGGAGTCTGTCCACGTCTATTACGAGAAGGTGGAGCCTCTGCTGAAGCTTGCGGGCATAGAGACTGATGT
GACAATAACAGAATATGAAGGGCATGCCCTGTCTCTGCTTGACGAATGTGAACTCCGAGGATTTGATGGT
GTTGTCTGTGTTGGTGGAGATGGATCTGCGAGTGAGGCCGCCCGCGCTTTGCTCCTCAGAGCTCAGAAGA
ACGCGGGAGTGGAGATGGACTGCATCCCGACTCTCGTTGGAGCAGAGCTTCCTCTTGGCTTCATTCCAGC
AGGATCTACTAACGCACTGGCACATTCTCTCTTTGGAACTCCTCACGTGGTGACTGCAACCATGCATATT
ATACTAGGTCACATACGGTCAGTGGATGTCTGCACCTTTAGCTCTGCTGGCAAGCTTCTTTGCTTTGGGT
TCTCAGCCATGTTTGGCTTTGGTGGAAGGACCTTGGCTCTGGCAGAGAAATACCGATGGATGTCCCCCAG
TCAGCGAAGAGACTTTGCTATAATCAAAGCACTGGCCAAACTTAAGCCAGAGGACTGCAAAATATCATTT
TTACTAGCAGGTTGTTCTCAGAATAAACAAGAAAGAAAATCACAGAGATCTGCAGAATCTGACTGTGGTG
GTCACTGGCAAACCATCCAGGGGCAGTTCTTGAACATCAGCATCATGGCAATGCCCTGTCCATGTTCAAT
GGCACCCAGAGGCTTTACACCTGACACCAGATTACATAATGGAAGCATGGCTCTTAGGGTTGTCCGGAAC
ACCACTCGATCAGAATTTGTAAAGCACCTGAAAAGACACTCCAGTGTAAAGAATCAGTTCAATTTTCCCT
TTGTGGAGACTTATATCATTGAAGAAGTAAAAATTCACCCAAAGAGTAATAGCAATGGATACAGCCCACA
GGAGGAGGGTGGAAACCATACAACTGCTTCAGAACACGGGTTTCCGTGGAATGTAGATGGTGACTTAATG
GAAGCTGCATCAGAGGTTCACATTAGGCTGCACCCAAGACTGATCAGGCTCTATGGAGGAAGCCTGGAAG
AAATGAATGATTGCAAAGTAGCATGTGATTGCATA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215371 representing NM_001048176
Red=Cloning site Green=Tags(s)

MFRGRRRSRVGALEEAPPENPKAAAEPERVLLRGIFEIRRESCDVALGERALRWRRIQPELPAGGTRHDL
LSKEESLELEDIFSVKLKRRCWAKHPGRDTLLGITLFICLKEQRNKLKDSTLDLINFSEDHCDIWFRQFK
KILDGFTSRPKALKILLNPQSHRKESVHVYYEKVEPLLKLAGIETDVTITEYEGHALSLLDECELRGFDG
VVCVGGDGSASEAARALLLRAQKNAGVEMDCIPTLVGAELPLGFIPAGSTNALAHSLFGTPHVVTATMHI
ILGHIRSVDVCTFSSAGKLLCFGFSAMFGFGGRTLALAEKYRWMSPSQRRDFAIIKALAKLKPEDCKISF
LLAGCSQNKQERKSQRSAESDCGGHWQTIQGQFLNISIMAMPCPCSMAPRGFTPDTRLHNGSMALRVVRN
TTRSEFVKHLKRHSSVKNQFNFPFVETYIIEEVKIHPKSNSNGYSPQEEGGNHTTASEHGFPWNVDGDLM
EAASEVHIRLHPRLIRLYGGSLEEMNDCKVACDCI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001048176
ORF Size 1575 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001048176.1, NP_001041641.1
RefSeq Size 1759 bp
RefSeq ORF 1578 bp
Locus ID 228094
Cytogenetics 2 C3
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Documents
Resources
"NM_001048176" in other vectors (4)
SKU Description Size Price
MC217516 Cerkl (untagged) - Mouse ceramide kinase-like (Cerkl), (10ug) 10 ug
$539.00
MR215371 Cerkl (Myc-DDK-tagged) - Mouse ceramide kinase-like (Cerkl) 10 ug
$289.00 $489.00
MR215371L3 Lenti ORF clone of Cerkl (Myc-DDK-tagged) - Mouse ceramide kinase-like (Cerkl) 10 ug
$789.00
MR215371L4 Lenti ORF clone of Cerkl (mGFP-tagged) - Mouse ceramide kinase-like (Cerkl) 10 ug
$789.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.