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Rdh12 (NM_030017) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215363
Rdh12 (tGFP-tagged) - Mouse retinol dehydrogenase 12 (Rdh12), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rdh12
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215363 representing NM_030017
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTTTATCTTGGTACTGCTTACGTCCTTCCTCTCCATCTTGTATCTGACAGCTCCATCCATCAGGA
AGTTCTTTGCTGGTGGAGTTTGTACAACAAATGTCCAGATCCCAGGGAAGGTAGTGGTCATCACAGGTGC
CAACACAGGCATTGGCAAGGAGACAGCCAGAGAGCTTGCTCGAAGAGGAGCACGAGTATACATTGCTTGC
CGAGATGTGCTGAAGGGAGAGTCTGCTGCTAGTGAAATCCGAGCAGATACCAAGAACTCCCAGGTGCTAG
TGCGGAAATTGGACCTGTCTGACACCAAATCCATCCGAGCCTTTGCTGAACGCTTCCTAGCAGAGGAAAA
GAAGCTTCATATTTTGATCAACAATGCAGGAGTGATGATGTGCCCATATTCTAAGACAACAGATGGCTTT
GAGACCCACTTTGGAGTCAACCACCTGGGACACTTTCTTCTTACATACCTGCTGTTGGAGAGGCTGAAGG
AGTCTGCTCCCGCACGGGTGGTCAACCTTTCCTCAATAGCTCACCTGATTGGCAAGATCCGTTTCCATGA
CCTCCAAGGCCAGAAACGATACTGCAGTGCTTTTGCCTATGGCCACAGCAAGCTGGCCAATCTGCTCTTC
ACTCGAGAACTGGCTAAGCGGCTCCAAGGGACTGGAGTCACCGCCTATGCGGTTCACCCGGGCGTTGTAT
TGTCAGAGATCACCAGGAACTCCTACCTGCTGTGTTTGCTATGGCGGCTCTTCTCACCCTTCTTCAAGTC
CACTTCTCAGGGGGCTCAGACCAGCCTGCACTGTGCTCTGGCGGAGGACCTAGAGCCCCTGAGTGGAAAG
TACTTCAGTGACTGCAAGAGGATGTGGGTATCCTCAAGGGCCCGGAACAAGAAAACAGCTGAGCGATTGT
GGAACGTCAGCTGCGAGCTTCTAGGAATCCAGTGGGAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215363 representing NM_030017
Red=Cloning site Green=Tags(s)

MLFILVLLTSFLSILYLTAPSIRKFFAGGVCTTNVQIPGKVVVITGANTGIGKETARELARRGARVYIAC
RDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAERFLAEEKKLHILINNAGVMMCPYSKTTDGF
ETHFGVNHLGHFLLTYLLLERLKESAPARVVNLSSIAHLIGKIRFHDLQGQKRYCSAFAYGHSKLANLLF
TRELAKRLQGTGVTAYAVHPGVVLSEITRNSYLLCLLWRLFSPFFKSTSQGAQTSLHCALAEDLEPLSGK
YFSDCKRMWVSSRARNKKTAERLWNVSCELLGIQWE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030017
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030017.4, NP_084293.1
RefSeq Size 1674 bp
RefSeq ORF 951 bp
Locus ID 77974
UniProt ID Q8BYK4
Cytogenetics 12 C3
Summary The protein encoded by this gene is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. The encoded enzyme also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Defects in the human gene are associated with Leber congenital amaurosis type 13, and Retinitis Pigmentosa 53. provided by RefSeq, Sep 2015
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Documents
Resources
"NM_030017" in other vectors (4)
SKU Description Size Price
MC211851 Rdh12 (untagged) - Mouse retinol dehydrogenase 12 (Rdh12), (10ug) 10 ug
$330.00
MR215363 Rdh12 (Myc-DDK-tagged) - Mouse retinol dehydrogenase 12 (Rdh12) 10 ug
$289.00 $300.00
MR215363L3 Lenti ORF clone of Rdh12 (Myc-DDK-tagged) - Mouse retinol dehydrogenase 12 (Rdh12) 10 ug
$600.00
MR215363L4 Lenti ORF clone of Rdh12 (mGFP-tagged) - Mouse retinol dehydrogenase 12 (Rdh12) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.