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Clca3b (NM_139148) Mouse Tagged ORF Clone

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SKU
MG215355
Clca4 (tGFP-tagged) - Mouse chloride channel calcium activated 4 (Clca4), (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
  $1,200.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Clca3b
Synonyms Clca4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215355 representing NM_139148, codon optimized.
Due to the complexity of NM_139148, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCCTGGCCTGCAAGTTCTGCTTTTCCTCACACTTCACCTGCTCCAAAACACAGAGAGTAGTATGG
TGCACCTGAACAGCAACGGATATGAAGGTGTTGTGATAGCAATTAACCCAAGCGTGCCTGAAGATGAGAG
GCTGATCCCGTCCATCAAGGAGATGGTGACACAGGCCAGTACCTATCTCTTCGAGGCTACAGAACGCCGC
TTTTATTTCCGAAATGTCTCCATACTTGTGCCCATTACCTGGAAAAGCAAGACCGAGTATCTCACACCTA
AACAGGAGTCCTACGACCAGGCAGACGTCATCGTAGCCGACCCACATCTGCAACATGGTGATGACCCCTA
TACTCTGCAGTATGGACAGTGTGGGGATAGGGGTCAATACATCCACTTTACGCCCAACTTTCTCCTGACC
GATAACCTCGGAATATATGGACCGCGCGGAAGGGTATTCGTTCACGAATGGGCCCATTTGCGGTGGGGCG
TGTTTGATGAATACAATATGGATAGACCATTCTATATGTCTAGAAAGAATACGGTCGAAGCTACTCGGTG
TTCCACCGATATAACAGGAACAAGTGTTGTGAGAGAGTGCCAGGGTGGCTCTTGCGTGTCTAGGCGGTGT
AGGAGGGACGCAAAAACCGGTATGCAAGAAGCGAAATGTACCTTCATACCCAATAAGTCCCAGACCGCTC
GGGGGAGCATTATGTTCATGCAGAGTCTTGATTCAGTGGTGGAGTTCTGTACAGAAAAAACTCATAATGT
GGAAGCACCTAATCTTCAGAATAAGATGTGTAACTTGCGATCTACTTGGGACGTCATTAAGGCCAGCGCA
GATTTCCAGAACGCCTCTCCAATGACCGGGACGGAGGCCCCGCCACTGCCTACTTTCTCTTTGCTGAAGT
CTCGCCAGCGGGTAGTGTGCCTGGTGCTGGACAAGAGCGGTTCAATGAGACTGGGCTCACCCATCACACG
GCTCACTCTGATGAACCAGGCCGCCGAGCTGTACCTGATTCAAATCATTGAGAAAGAGAGTCTTGTGGGT
TTGGTAACCTTTGACTCAACCGCTACCATCCAGACTAACCTCATCCGCATAATTAACGACTCCTCTTACC
TTGCAATTTCTACCAAACTCCCACAATATCCCAACGGCGGGACGTCCATCTGTAATGGACTGAAGAAGGG
CTTCGAGGCGATTACTAGCTCCGACCAATCCACATCTGGCTCCGAGATCGTGCTGCTGACAGACGGTGAA
GACAACCGAATCAGCTCCTGCTTCCAGGAAGTCAAACATAGTGGCGCCATCATCCATACCATTGCACTGG
GCCCTTCCGCTGCACGAGAACTGGAAACCCTGAGTGATATGACCGGAGGATTGAGATTTTACGCAAAAGA
GGATGTTAATGGGCTCATCGATGCATTTTCTGGTATCTCCTCTAAGTCAGGGTCCATCTCACAGCAAGCA
CTTCAGCTGGAAAGCAAAGCTTTCAATGTGGGCGCGGGGGCGTGGATCAACAGTACCGTGCCTGTCGACT
CTACCGTGGGCAACGATACCTTCTTCGTGATTACATGGACGGTCAGAAAGCCCGAAATCATCCTGCAGGA
CCCCAAGGGGAAGAACTACACCACATCTGATTTCCAGGAGGACAAGCTCAACATCTTCAGCGTGCGCCTG
AGGATTCCCGGCATTGCAGAGACTGGAACATGGACGTATAGTCTGTTGAATAAAGGCGCTACTAGTCAAC
TGCTTACAGTAACGGTGACTACCCGCGCGAGGAGCCCCACAACATTGCCCGTCATTGCGACAGCCCACAT
GTCCCAATCCACCGCCCAATATCCCTCAAGGATGATCGTGTACGCCAGAGTGAGTCAGGGGTTCTTGCCT
GTGCTTGGAGCTAACGTCACCGCCGTAATCGAGGCGGAGTCTGGCAATCAGGTTACCTTGGAATTGTGGG
ACAATGGAGCTGGAGCTGACACTCTCAAGAACGACGGCATCTACTCACGATACTTTACAGATTTTCACGG
TAACGGGCGCTACTCTCTGAAGGTGAACGCTCAGGCAAGAAAGAACATGGCGAAACTGAACCTGAAGCAG
AAGAACAAATCCCTCTATATCCCCGGGTACGTGGAAAACGACCAAATTGTTCTGAATCCTCCTCGCCCGG
AAATCCCTGAGGCCACCGAAGCCACCGTGGAGGACTTTTCTCGCCTGACCAGTGGCGGGAGCTTTACCGT
CTCCGGAGCACCCCCTGACGGAGATCATGCAAGGGTTTTCCCTCCTAGTAAGGTGACCGATTTGGAGGCT
GAGTTTATTGGGGATCATATTCATCTCACATGGACCGCTCCCGGCAAGGTTCTTGACAAAGGTCGCGCTT
ATCGCTATGTCATCCGCATGTCTGGCCATTCCTTGGCGCTGCAGGAGGATTTCTCTAACAGCACCCTTGT
CAACACATCTTCTGTAATGCCCAAGGAGGCAGGGTCTAAGGAAACCTTCAAATTCAAGCCTGAGACATTT
AAGATCGAGAACGGCACTCAGGTGTACATAGCAATCCAGGCCGATAACGAGGCCAGACTGTCCTCTGAGG
TGTCTAACATCGCACAGGCCGTAAAGTTCATCCCCCCACAGGTGTATCTTACTCCTAGTACCCCCCCAGG
TCTGTCCACCCCGAGTACCCCTCCCGGCCTGAGTACACCTAGTACTCCACCCGGCCTGAGCACCCCTTCA
ACACCCCCTGGACTGTCTACTCCATCTACTCCCCCCGGGCTCTCCACTCCTTCAACACCTCCGGGATTGT
CCACCCCATCCACCCCACCGGGTCTGAGTACTCCCTCCACTCCGCCAGGCTTGTCTACCCCTTCCACTCC
TCCCGGGCTGTCAACTCCCAGTACGCCACCTGGTCTGAGCACTCCATCTACCCCTCCTGGCCTGTCAACG
CCGTCCACCCCTCCAGGATTGAGCACTCCTAGCACTCCTCCCGGGCTGAGCACTCCCTCAACCCCCCCTG
GTCTGAGCACCCCCAGTACTCCTCCAGGCCTCTCCACCCCCAGCACGCCACCCGGTTTGGGCACAAAAGT
TAGCGTGCCCTCACTGACAGTCTTCGTCCTGGTGGCCACCTTGTTTATTTTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215355 representing NM_139148
Red=Cloning site Green=Tags(s)

MVPGLQVLLFLTLHLLQNTESSMVHLNSNGYEGVVIAINPSVPEDERLIPSIKEMVTQASTYLFEATERR
FYFRNVSILVPITWKSKTEYLTPKQESYDQADVIVADPHLQHGDDPYTLQYGQCGDRGQYIHFTPNFLLT
DNLGIYGPRGRVFVHEWAHLRWGVFDEYNMDRPFYMSRKNTVEATRCSTDITGTSVVRECQGGSCVSRRC
RRDAKTGMQEAKCTFIPNKSQTARGSIMFMQSLDSVVEFCTEKTHNVEAPNLQNKMCNLRSTWDVIKASA
DFQNASPMTGTEAPPLPTFSLLKSRQRVVCLVLDKSGSMRLGSPITRLTLMNQAAELYLIQIIEKESLVG
LVTFDSTATIQTNLIRIINDSSYLAISTKLPQYPNGGTSICNGLKKGFEAITSSDQSTSGSEIVLLTDGE
DNRISSCFQEVKHSGAIIHTIALGPSAARELETLSDMTGGLRFYAKEDVNGLIDAFSGISSKSGSISQQA
LQLESKAFNVGAGAWINSTVPVDSTVGNDTFFVITWTVRKPEIILQDPKGKNYTTSDFQEDKLNIFSVRL
RIPGIAETGTWTYSLLNKGATSQLLTVTVTTRARSPTTLPVIATAHMSQSTAQYPSRMIVYARVSQGFLP
VLGANVTAVIEAESGNQVTLELWDNGAGADTLKNDGIYSRYFTDFHGNGRYSLKVNAQARKNMAKLNLKQ
KNKSLYIPGYVENDQIVLNPPRPEIPEATEATVEDFSRLTSGGSFTVSGAPPDGDHARVFPPSKVTDLEA
EFIGDHIHLTWTAPGKVLDKGRAYRYVIRMSGHSLALQEDFSNSTLVNTSSVMPKEAGSKETFKFKPETF
KIENGTQVYIAIQADNEARLSSEVSNIAQAVKFIPPQVYLTPSTPPGLSTPSTPPGLSTPSTPPGLSTPS
TPPGLSTPSTPPGLSTPSTPPGLSTPSTPPGLSTPSTPPGLSTPSTPPGLSTPSTPPGLSTPSTPPGLST
PSTPPGLSTPSTPPGLSTPSTPPGLSTPSTPPGLSTPSTPPGLGTKVSVPSLTVFVLVATLFIF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_139148
ORF Size 3132 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139148.2, NP_631887.2
RefSeq Size 3306 bp
RefSeq ORF 3135 bp
Locus ID 229927
Cytogenetics 3 H2
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Documents
Resources
"NM_139148" in other vectors (2)
SKU Description Size Price
MC223337 Clca4 (untagged) - Mouse chloride channel calcium activated 4 (Clca4), (10ug) 10 ug
$1,001.00
MR215355 Clca4 (Myc-DDK-tagged) - Mouse chloride channel calcium activated 4 (Clca4). Note: ORF is codon optimized 10 ug
$1,000.00

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