Cpa6 (NM_177834) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG215354
Cpa6 (tGFP-tagged) - Mouse carboxypeptidase A6 (Cpa6), (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cpa6
Synonyms 9030616D13Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215354 representing NM_177834
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTTCCTCGGGAATCCCAGGAGCCACACAGCAGCTTTTCTGCCGGTTTGCTGGCTCCTTTTGAATA
TTCTGAAACCTGGGCACTGCCACTCTTACGACAACCGCTATGTTGGTGATAAAGTGATAAGACTCATCCC
CAAATCCGAAGAGGAAGCACTTGCACTCAAGAACATATACCACCAACTTAAGGTGGACCTGTGGCAGCCC
AGCAGTATCTCCTATGTGTCAGAGGGGACAATTACTGATGTCCATATTTCCCAAAATGCTTCCAGAACCC
TGTTAGCGTTCTTACAGGAAACCCACATCTACTACAAGGTCCTCATAGAAGATCTTCAGAAAGCTGTGGA
AAATGAAAACAGCTTGCAAACCCAGAGAAACAGAAGATCCCTGTCTGAATACAACTACGAGGTGTATCAC
TCCTTAGAAGATATTCAGAGTTGGTTGCACCATCTGAACCAAACTCAGCCAGGCCTCGTTCGAGTGTTCT
CTATTGGAAGATCATACGAAGGAAGACCTCTCTTTATTATGCAGCTGGGCAGAAAATCACGAGCCTACAA
AAGAGCTGTCTGGATAGACTGCGGCATTCACGCAAGAGAATGGATCGGTCCTGCCTTTTGTCAGTGGTTT
GTAAGAGAAGCCATTCTGACCTACAAGACTGACCCAGCCATGAAAAAAATGTTGAATCATCTGTATTTCT
ATATCATGCCTGTATTCAATGTCGATGGATACCATTTCAGCTGGACACATGATAGATTCTGGAGAAAGAC
CCGGTCAAGAGACTCTAAGTTTCGCTGTCGTGGAGTTGATGCCAATCGCAACTGGAAAGTGAAATGGTGC
GATGAAGGAGCCTCCGCACACCCTTGTGATGATACATACTGTGGTCCTTTCCCAGAATCTGAACCAGAAG
TGAAGGCTGTAGCTAACTTCCTTCGAAAACACAGAAAGCGCATCAGGGCGTACCTGTCTTTTCATGCCTA
TGCTCAGATGTTGCTATATCCTTATTCTTACAAATATGCAACAATCCCCAATTTTAGCTGTGTGGAATTT
GCAGCTCACAAAGCTGTGAAGGCACTTCGGTCGGTACATGGGATCCGATACAGACATGGCCCCGCCTCCC
AAACACTGTATGTAAGCTCTGGTAACTCAATGGATTGGGCTTATAAAAATGGAATACCCTATGCATTTGC
TTTTGAACTGCGAGACACTGGGCATTTTGGATTTCTACTGCCAGAGATGCTCATTAAACCCACCTGCACA
GAGACCATGCTGGCTGTGAAGAATATCACAATGCACCTGCTAAAGAAATGTCCT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215354 representing NM_177834
Red=Cloning site Green=Tags(s)

MNFLGNPRSHTAAFLPVCWLLLNILKPGHCHSYDNRYVGDKVIRLIPKSEEEALALKNIYHQLKVDLWQP
SSISYVSEGTITDVHISQNASRTLLAFLQETHIYYKVLIEDLQKAVENENSLQTQRNRRSLSEYNYEVYH
SLEDIQSWLHHLNQTQPGLVRVFSIGRSYEGRPLFIMQLGRKSRAYKRAVWIDCGIHAREWIGPAFCQWF
VREAILTYKTDPAMKKMLNHLYFYIMPVFNVDGYHFSWTHDRFWRKTRSRDSKFRCRGVDANRNWKVKWC
DEGASAHPCDDTYCGPFPESEPEVKAVANFLRKHRKRIRAYLSFHAYAQMLLYPYSYKYATIPNFSCVEF
AAHKAVKALRSVHGIRYRHGPASQTLYVSSGNSMDWAYKNGIPYAFAFELRDTGHFGFLLPEMLIKPTCT
ETMLAVKNITMHLLKKCP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177834
ORF Size 1314 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177834.3, NP_808502.2
RefSeq Size 1930 bp
RefSeq ORF 1317 bp
Locus ID 329093
UniProt ID Q5U901
Cytogenetics 1 A2
Summary This gene encodes a protein that belongs to the metallocarboxypeptidase family of proteins that catalyze the release of a C-terminal amino acid from the target protein. The encoded preproprotein undergoes proteolytic cleavage to yield the mature form which is thought to play a role in cell migration. In humans, this protein regulates neuropeptides in the brain and mutations in this gene are associated with a recessive familial form of febrile seizures and with temporal lobe epilepsy. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Jan 2014
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Documents
Resources
"NM_177834" in other vectors (4)
SKU Description Size Price
MC215888 Cpa6 (untagged) - Mouse carboxypeptidase A6 (Cpa6), (10ug) 10 ug
$503.00
MR215354 Cpa6 (Myc-DDK-tagged) - Mouse carboxypeptidase A6 (Cpa6) 10 ug
$289.00 $457.00
MR215354L3 Lenti ORF clone of Cpa6 (Myc-DDK-tagged) - Mouse carboxypeptidase A6 (Cpa6) 10 ug
$757.00
MR215354L4 Lenti ORF clone of Cpa6 (mGFP-tagged) - Mouse carboxypeptidase A6 (Cpa6) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.