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Cers3 (NM_001164201) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215337
Cers3 (tGFP-tagged) - Mouse LAG1 homolog ceramide synthase 3 (Lass3), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Cers3 or NM_001164201"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cers3
Synonyms 4930550L11Rik; Gm488; Lass3; T3L
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215337 representing NM_001164201
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGTGTGTTGCTCTTGAGAACAAAGCTGCCTGGAACGGGAAGCCTCTCCAGTTGCTCCCCACAGGCG
AGGAGTATCCTGTGCAGACTGGCGCTTTGGGAAGAAGAATGTTTCAGACGTTTAGAAAATGGTTCTGGTC
GGAGAGATACTGGCTTCCTCCAACAATAAAGTGGTCAGACCTGGAAGATCATGATGGGCTTGTCTTCGTG
AAAGCTTCCCACTTATACATCACAATTCCATATGCCTTTCTTTTAATGGTCGTCAGATACTTCTTTGAAA
AGTTTGTTGCTACACCTCTAGCAAATGCACTTGGCATTAAAAAGACACAGCACAAAATTAAACCAAATGC
CATCTTAGAGAATTTTTTCAAGCATTCCACAAGCAAACCATCACATACTGATATTTATGGACTGGCAAAG
AAGTGTAACTTGACAGAGCGCCAGGTTGAAAGATGGCTTAGGATTCGGCAGAAGCAAAACAAGCCTTGCA
GGCTGCAGAAATTCCAGGAGTCTTGCTGGCGATTTACATTTTACTTGCTGATAACCATGGCTGGAGCTGT
ATTTCTGTATGACAAACCATGGGCATATGACCTCTGGGAGGTTTGGAATGACTATCCCAGACAGCCTTTG
CTGCCGTCCCAGTACTGGTACTACATTTTGGAGATGAGTTTTTATTGGTCACTGGTGTTTAGCCTGAGCA
CTGATATCAAGAGGAAGGACTTCCTCGCTCATGTCATCCACCACCTGGCTGCTATTAGTCTGATGAGCTT
CTCCTGGTGTGCTAATTACATTCGCAGTGGGACCCTCGTGATGTTTATTCATGATATATCTGACATTTGG
CTAGAGTCTGCTAAGATGTTTTCCTATGCTGGATGGAAGCAGACGTGTAACACCCTGTTCTTCATCTTCA
CCGTCGTCTTCTTCATTAGCCGATTCATTATTTTTCCATTCTGGATTCTGTACTGCACTCTGATCCTGCC
TTTGCACTATCTCGAGCCCTTCTTCTCCTACATCTTCCTCAACCTCCAACTCATGATCCTTCAGGGCCTC
CACGTTTACTGGGGTTACTTTATCCTGAAGATGCTCAACAGATGCATATTCACGCAGAACGTCCAGGATG
TGAGGAGTGACAACGAAGAGGAAGAGGAAGAGGAGGAGGAAGAGGAAGCAGAGAGCACCAAGGGCAAAGA
GACAGAGTATTTGAAGAATGGCCTTGGGACCAACAGGCATCTCATTGCCAATGGTCAGCATGGCCGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215337 representing NM_001164201
Red=Cloning site Green=Tags(s)

MRCVALENKAAWNGKPLQLLPTGEEYPVQTGALGRRMFQTFRKWFWSERYWLPPTIKWSDLEDHDGLVFV
KASHLYITIPYAFLLMVVRYFFEKFVATPLANALGIKKTQHKIKPNAILENFFKHSTSKPSHTDIYGLAK
KCNLTERQVERWLRIRQKQNKPCRLQKFQESCWRFTFYLLITMAGAVFLYDKPWAYDLWEVWNDYPRQPL
LPSQYWYYILEMSFYWSLVFSLSTDIKRKDFLAHVIHHLAAISLMSFSWCANYIRSGTLVMFIHDISDIW
LESAKMFSYAGWKQTCNTLFFIFTVVFFISRFIIFPFWILYCTLILPLHYLEPFFSYIFLNLQLMILQGL
HVYWGYFILKMLNRCIFTQNVQDVRSDNEEEEEEEEEEEAESTKGKETEYLKNGLGTNRHLIANGQHGR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001164201
ORF Size 1257 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001164201.1, NP_001157673.1
RefSeq Size 3251 bp
RefSeq ORF 1260 bp
Locus ID 545975
UniProt ID Q1A3B0
Cytogenetics 7 C
Summary Has (dihydro)ceramide synthesis activity with relatively broad substrate specificity, but a preference for C18:0 and other middle- to long-chain fatty acyl-CoAs. It is crucial for the synthesis of very long-chain ceramides in the epidermis, to maintain epidermal lipid homeostasis and terminal differentiation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001164201" in other vectors (4)
SKU Description Size Price
MC215129 Cers3 (untagged) - Mouse LAG1 homolog, ceramide synthase 3 (Lass3), (10ug) 10 ug
$503.00
MR215337 Cers3 (Myc-DDK-tagged) - Mouse LAG1 homolog, ceramide synthase 3 (Lass3) 10 ug
$289.00 $457.00
MR215337L3 Lenti ORF clone of Cers3 (Myc-DDK-tagged) - Mouse LAG1 homolog, ceramide synthase 3 (Lass3) 10 ug
$757.00
MR215337L4 Lenti ORF clone of Cers3 (mGFP-tagged) - Mouse LAG1 homolog, ceramide synthase 3 (Lass3) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.