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Synb (NM_173420) Mouse Tagged ORF Clone

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SKU
MG215334
Synb (tGFP-tagged) - Mouse RIKEN cDNA D930020E02 gene (D930020E02Rik), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $776.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Synb
Synonyms D930020E02Rik; syncytin-B
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215334 representing NM_173420
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGGCTTTTGGGTCCTCTGTTTCGTCCTTTTCCCCTCCTCCTTATCCTATCCGGAAAGCTGGATGC
CCCTTGTAAACCTCACTCACCACATCCTACGTGATACCAACTCTTCCCTGTTTTCCAACTGTTGGGTCTG
CTTGTCTACCCAAACCCAGCGGTCCTTAGCAGTCCCAGCCCCTCTGTCCATTTGGACAGATACACCCATG
AAGCTTCATCTTACCTACTCAGTCAGGCCCTTCTCTGGCTCCTTTTCCATTAGCGACATTGAAAGACGCC
TCCGTCTCTTCCGCCCACTGACTGCCTCCTATTCTTTCCACAATCCTGACAGAAGGGCGATTGCTTTTCT
TCAACTCGTCAGCTCAACAGGCATATTTCGGATCATCACCCGGATAACCTCTGTGATATATCCCCATAAG
GACCGTTTCTTCGAATCTGCCCAACGCCCTCTCTGGGGACCACTCTTTACTGAGACCGTGCTCAGGTCGC
AGGCCCCACTCTGCATATCTCGCTTTTTCAAGGTCTCAGCATATGCCACTTTTGTAGGCAACCTCTCTGC
CTCTCTCTGCAACTACACCATGCATATTTCACCTTCTACCAGTCATGAAAACCTAGATCTTTCCACCACC
CATACGTTCAAACAGGCAATGAAAAGACCGGATGCCAAATGGAAAAACCCGCTCCGTTTTTCCGGGCCCC
CCTCCCTCATCTTCTCGAAGCCGGCTTACTATCCCTGCCCAACAGACATCAAACACTGCCATACCTCTCC
GGCCACTCCCTGGATGCACTGTCCTCAGGCTCCCTTCGGCACCTGCTATAACCTCACTTTATTTGAACCA
GACAACTCAACCCACCCTGTTACCATGTCAGTGAACCCTACCCACTTCAAGGTCAAACTCCAGGGGCACA
GAGACCCCTATCCGCTCTCCCATTACCAGCCCCTCACGGGAGCTGCCCTGTCTGGACAATATTCAGTCTG
GGAGAACGAGATCACTGTCCAAGAAAACTGGGACATCACCTCCAACATTTTCTCACATCTTCTCAGCTTC
TCGTACGCCTTCTGCCTCAACTCTTCAGGCGTTTTCTTCCTCTGCGGAACATCGACTTACATCTGCCTCC
CAGCCAATTGGTCCGGTGTCTGTACCCTGGTCTTCCAATACCCGGATATTGAACTTCTCCCCAATAACCA
AACGGTGCCTGTTCCCCTTTTTGCTTCAGTTCTTTCCTCAGACTCAGTTCTTCGCCCAAAGAGGTCCCCT
CACCTCTTTCCCTTCCTTGCAGGCCTGGGTATCTCTTCTGCCCTTGGTACGGGGATAGCTGGCTTGGCCA
CCTCGACTCTCTATTTCCAACAGCTTTCTAAGGTTCTTTCCGAAACCTTGGAAGAAATAGCTGCCTCTAT
CACTACCCTCCAGAACCAAATAGACTCGCTCGCAGGTGTTGTTCTACAAAACCGCCGAGCTCTGGACCTC
ATCACTGCTGAGAAAGGGGGCACCTGTCTCTTCCTCCAGGAAGAGTGCTGCTTCTACGTAAACCAGTCTG
GAATAGTCCGGGACGCGGCAAGGAAACTCCAAGAACGAGCATCTGAACTCGGCCAGCATTCTGACTCTTG
GGGACAGTGGCCTGACCTTGGACGTTGGTTGCCCTGGCTGACTCCCTTTCTGGGACCTCTTCTCTTCCTC
TTCTTCCTACTGACATTTGGGTCTTGTCTTCTGAACTGCCTAACCCGTTTTGTGTCCCAGAGACTTGGCT
CCTTTGTTCAAGACACTGCCAAAAGGCATGTGGACAGCATCCTCCAAAATTTCCAATATAAAAAACTGCC
CCAAGACTCCCCAGATGAGGACACCATTCCTACA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215334 representing NM_173420
Red=Cloning site Green=Tags(s)

MTGFWVLCFVLFPSSLSYPESWMPLVNLTHHILRDTNSSLFSNCWVCLSTQTQRSLAVPAPLSIWTDTPM
KLHLTYSVRPFSGSFSISDIERRLRLFRPLTASYSFHNPDRRAIAFLQLVSSTGIFRIITRITSVIYPHK
DRFFESAQRPLWGPLFTETVLRSQAPLCISRFFKVSAYATFVGNLSASLCNYTMHISPSTSHENLDLSTT
HTFKQAMKRPDAKWKNPLRFSGPPSLIFSKPAYYPCPTDIKHCHTSPATPWMHCPQAPFGTCYNLTLFEP
DNSTHPVTMSVNPTHFKVKLQGHRDPYPLSHYQPLTGAALSGQYSVWENEITVQENWDITSNIFSHLLSF
SYAFCLNSSGVFFLCGTSTYICLPANWSGVCTLVFQYPDIELLPNNQTVPVPLFASVLSSDSVLRPKRSP
HLFPFLAGLGISSALGTGIAGLATSTLYFQQLSKVLSETLEEIAASITTLQNQIDSLAGVVLQNRRALDL
ITAEKGGTCLFLQEECCFYVNQSGIVRDAARKLQERASELGQHSDSWGQWPDLGRWLPWLTPFLGPLLFL
FFLLTFGSCLLNCLTRFVSQRLGSFVQDTAKRHVDSILQNFQYKKLPQDSPDEDTIPT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173420
ORF Size 1854 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173420.3, NP_775596.1
RefSeq Size 3001 bp
RefSeq ORF 1857 bp
Locus ID 239167
UniProt ID Q8BI41
Cytogenetics 14 D2
Summary Many different endogenous retrovirus families are expressed in normal placental tissue at high levels, suggesting that endogenous retroviruses are functionally important in reproduction. This gene is part of an endogenous retrovirus provirus that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product plays a major role in placental development and trophoblast fusion. The protein has the characteristics of a typical retroviral envelope protein, including a cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. provided by RefSeq, Apr 2015
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Documents
Resources
"NM_173420" in other vectors (4)
SKU Description Size Price
MC219767 Synb (untagged) - Mouse RIKEN cDNA D930020E02 gene (D930020E02Rik), (10ug) 10 ug
$576.00
MR215334 Synb (Myc-DDK-tagged) - Mouse RIKEN cDNA D930020E02 gene (D930020E02Rik) 10 ug
$289.00 $576.00
MR215334L3 Lenti ORF clone of Synb (Myc-DDK-tagged) - Mouse RIKEN cDNA D930020E02 gene (D930020E02Rik) 10 ug
$876.00
MR215334L4 Lenti ORF clone of Synb (mGFP-tagged) - Mouse RIKEN cDNA D930020E02 gene (D930020E02Rik) 10 ug
$876.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.