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Nxf2 (NM_031259) Mouse Tagged ORF Clone

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SKU
MG215333
Nxf2 (tGFP-tagged) - Mouse nuclear RNA export factor 2 (Nxf2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $890.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nxf2
Synonyms 4930455N06Rik; Nxf-b
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215333 representing NM_031259
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGTCCTCTCCAAAAGAGAATCTGCAAGGCAGGTCTTCCATGTTTGTTCAGAAGAACATTAATTCTG
AGACATATAAACAACGATATGGTTTACCATACAAGAGATCTGAGAGATTCTATCATTCAGAATACAAAAT
GAATTATAACCATGGTTTTCAAGGAAGAAAGAGAGGTGTGAATTATATCTGGAGTCAATTTGACAGAAAG
AACAATCATTTTGATCATTATGGTGCTCCATATGCCATGGGAATGAAAAGAAGGAGAGAAAGATGCAGTT
ATGATGACCAATATTTTCTTAATGTGTGGGATGATAGTAAAACTGAGGAGGGCGAAACAGATCTGGATGC
TGAAAATGAAACTGAGGAGAAATGGTACAAGGTCACAATTCCCAGTGGAAGAAAGTATGAGAAGACATGG
CTAATGAGGTCAATCCAGAACTTCTGTAGTGAGCCCTTCATCCCTGTTGATTTCCACTATGACAAAACCC
AGGCCCGGTTCTTTGTTCAGAATGCTAAGACTGCCTCTGCATTGAAGGATGTCAGCTACAGGATTTGTGA
TGAAACAAGCAGAAAAATAGCGATCTTTGTCAGTCCTTCTGTTGTGCCCTATTCTGTGCAAAACAAGTTT
ACATCAGAACAAATGGAGTACATAAGGGAATCTATGATGAACCGGTATGACGCCTCCCAGAAAGCTCTGG
ACCTCGAAAAGTTCCGATTTGACCAGGACTTAATGGACAAGGATATTGACATGATGCTGAATCGAAGAAG
CTGCATGGTTGCCACACTACAGATCATTCAAAGTGATATCCCTGAACTGTTGTCCTTGAACTTGACAAAC
AACAAACTGTACCAGCTGGATGGGCTGTCAGACATGACAGAGAAGGCCCCTCACGTTAAGATCCTGAACC
TCTCCCGAAATAAACTGAAGTCATTCACGGAATTGGAGAAGGTGAAAGAACTGAAGCTGGAAGAGCTGTG
GCTGGAAGGGAACCCCTTCTGCAACTGCTTCTTGGATCATTTTGAGTATATAAGTACTATTCATGACCTA
TTCCCCAAGCTGTTGCGTCTGGATGGCGAGGACATAATAGTACCAAAAAGAAATCTTCAGAATGGCAAGG
GCCTAATAGTACCGACAAGAAATCTTCAGAATGGCAAGGACTTAATAGTACCGACAGGAAATCCTCAGGA
TGGCAAGGACTTAATAGTACCGACAGGAAATCCTCAGGATGGCAAGGACCTAATAGTACCAACAGGAAAT
CCTCAGGATGGCAAGGACCTAATAGTACCAACAAAAATGGACATCGAGGTCCCGCAACCATGCAAGGAAA
GCTGTAACACATCTGAAGTCATAAAAAATCTAGTTCTACAATTTCTGAAAGAGTACTACTTGTTTTATGA
CAATGGAGATCGACTTCGTCTTCTCGATGCTTACCATGACCAGGCCTGCTTCTCCTTGTCAGTTCCTTTC
GATGTCAGTGACCCAAACCTGAACAACTTGGAAGAGTATTTCAAATACAGCAGAGATCTAAAGAGGCAGC
AAGACTCAAGCATGCGAATGCAGTTGCTGAAGCACACAAAACATGACATCGTGAACTCTCTTAGCTTGTT
ACCCAAAACTCAGCATGATCTTTGCTCTTTCTTGGTGGACTTGTTTCTCCACACGGAAATGATGCTCTGT
TTTTCTGTGAATGGACTATTCATGGAAGTGGAAGGAAAATGTCGAGGCTGCATCCGTGCCTTCACGAGGA
TCTTCATTGCTATCCCTTGCAGCGATTCAAGAATTTGCATCATGAACGATGAGCTGATTGTGAGGAATGC
CAGTCCCAAAGAGATACAAAAGGCCTTCACCTCATTGCCTGCACCCGACACGTCATTCAAGCCTTTGCTC
TCTGAAGAACAGCAGGAAATGGTGAAGTCTTTCTCTGTGCAGTCTGGAATGAAACTTGACTGGTCTCAGA
AGTGCCTTCAAGATAACGAGTGGGACTACACCAAAGCTGGTGAGGCCTTCACTGCTCTCCAGAATGAGGG
CAAGATCCCAAAGGAATTCTTCAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215333 representing NM_031259
Red=Cloning site Green=Tags(s)

MWSSPKENLQGRSSMFVQKNINSETYKQRYGLPYKRSERFYHSEYKMNYNHGFQGRKRGVNYIWSQFDRK
NNHFDHYGAPYAMGMKRRRERCSYDDQYFLNVWDDSKTEEGETDLDAENETEEKWYKVTIPSGRKYEKTW
LMRSIQNFCSEPFIPVDFHYDKTQARFFVQNAKTASALKDVSYRICDETSRKIAIFVSPSVVPYSVQNKF
TSEQMEYIRESMMNRYDASQKALDLEKFRFDQDLMDKDIDMMLNRRSCMVATLQIIQSDIPELLSLNLTN
NKLYQLDGLSDMTEKAPHVKILNLSRNKLKSFTELEKVKELKLEELWLEGNPFCNCFLDHFEYISTIHDL
FPKLLRLDGEDIIVPKRNLQNGKGLIVPTRNLQNGKDLIVPTGNPQDGKDLIVPTGNPQDGKDLIVPTGN
PQDGKDLIVPTKMDIEVPQPCKESCNTSEVIKNLVLQFLKEYYLFYDNGDRLRLLDAYHDQACFSLSVPF
DVSDPNLNNLEEYFKYSRDLKRQQDSSMRMQLLKHTKHDIVNSLSLLPKTQHDLCSFLVDLFLHTEMMLC
FSVNGLFMEVEGKCRGCIRAFTRIFIAIPCSDSRICIMNDELIVRNASPKEIQKAFTSLPAPDTSFKPLL
SEEQQEMVKSFSVQSGMKLDWSQKCLQDNEWDYTKAGEAFTALQNEGKIPKEFFK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_031259
ORF Size 2055 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031259.2, NP_112549.1
RefSeq Size 2381 bp
RefSeq ORF 2058 bp
Locus ID 83454
Cytogenetics X 56.31 cM
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Documents
Resources
"NM_031259" in other vectors (2)
SKU Description Size Price
MC220448 Nxf2 (untagged) - Mouse nuclear RNA export factor 2 (Nxf2), (10ug) 10 ug
$691.00
MR215333 Nxf2 (Myc-DDK-tagged) - Mouse nuclear RNA export factor 2 (Nxf2) 10 ug
$690.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.