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Gm4836 (NM_009529) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215300
Gm4836 (tGFP-tagged) - Mouse predicted gene 4836 (Gm4836), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gm4836
Synonyms 22526
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215300 representing NM_009529
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTATTAAGAAACTGTGGGTGATACCAAAGGATGGTTACTTATTACTTCTTGACTATGATTCAGATG
AAGAAGAAGAGCAGGCTCATTCTGAAGTAAAGAGGCCAGCATTTGGCAAACATGAGAATATGCCGCCTCA
CGTAGAAGCAGATGAAGATATAAGAGATGAACAAGACAGTATGTTGGATAAATCTGGAGAAAACGTAAGT
TTCTCAGAGGAATGGCAGCGTTTTGCACGTTCTGTAGAGACACCAATGGAAAACTGGAATTTGTTAAGTG
GTGAACAGCAAGTTAGGAATGCTTCAGAATTGGACCTTATGGAAGTACAGAATCCAGTAACTCATGATGA
TGGGAATGCAAATCCTGAAGAAGTAGTTGGAGATACACGAAAGAAGATCAACAACAAATTGTGTGAGCAG
AAGTTTGATATGGATATACAGAAATTCAATGAAGAACAAGAAAAATCAGTGAACAATTATCAAAAAGAAC
AACAAGCATTAAAACTGTCCGAATGTAGTCAGAGTCCGACCATGGAAGCAATTGAAGACATGCATGAGAA
GTCCATGGAGGGTTTGATGAACATGGAGACCAACAACTACGATATGCTTTTTGATGTAGATGGTGAAGAG
ACATTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215300 representing NM_009529
Red=Cloning site Green=Tags(s)

MSIKKLWVIPKDGYLLLLDYDSDEEEEQAHSEVKRPAFGKHENMPPHVEADEDIRDEQDSMLDKSGENVS
FSEEWQRFARSVETPMENWNLLSGEQQVRNASELDLMEVQNPVTHDDGNANPEEVVGDTRKKINNKLCEQ
KFDMDIQKFNEEQEKSVNNYQKEQQALKLSECSQSPTMEAIEDMHEKSMEGLMNMETNNYDMLFDVDGEE
TL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009529
ORF Size 636 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009529.3, NP_033555.1
RefSeq Size 917 bp
RefSeq ORF 639 bp
Locus ID 22526
Cytogenetics X
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Documents
Resources
"NM_009529" in other vectors (4)
SKU Description Size Price
MC209660 Gm4836 (untagged) - Mouse predicted gene 4836 (Gm4836), (10ug) 10 ug
$330.00
MR215300 Gm4836 (Myc-DDK-tagged) - Mouse predicted gene 4836 (Gm4836) 10 ug
$289.00 $300.00
MR215300L3 Lenti ORF clone of Gm4836 (Myc-DDK-tagged) - Mouse predicted gene 4836 (Gm4836) 10 ug
$600.00
MR215300L4 Lenti ORF clone of Gm4836 (mGFP-tagged) - Mouse predicted gene 4836 (Gm4836) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.