Cort (NM_007745) Mouse Tagged ORF Clone
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Cort |
| Synonyms | CS; CST; PC; PCST |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007745 |
| ORF Size | 327 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007745.4, NP_031771.1 |
| RefSeq Size | 498 bp |
| RefSeq ORF | 330 bp |
| Locus ID | 12854 |
| UniProt ID | P56469 |
| Cytogenetics | 4 78.89 cM |
| Summary | This gene encodes a member of the somatostatin family of multifunctional peptides attributed with neurohormone, neurotransmitter/modulator and autocrine/paracrine actions. The encoded preproprotein undergoes proteolytic processing to generate a mature functional peptide that can bind to somatostatin receptors. Mice lacking the encoded protein exhibit elevated levels of growth hormone in the plasma without major changes in somatic growth and have exacerbated nociceptive responses to neuropathic and inflammatory pain sensitization. Transgenic mice overexpressing the encoded protein in neurons do not express long-term potentiation in the dentate gyrus and exhibit deficits in synaptic plasticity and learning. provided by RefSeq, Nov 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007745" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC208307 | Cort (untagged) - Mouse cortistatin (Cort), (10ug) | 10 ug |
$165.00
|
|
| MR215280 | Cort (Myc-DDK-tagged) - Mouse cortistatin (Cort) | 10 ug |
$289.00
|
|
| MR215280L3 | Lenti ORF clone of Cort (Myc-DDK-tagged) - Mouse cortistatin (Cort) | 10 ug |
$450.00
|
|
| MR215280L4 | Lenti ORF clone of Cort (mGFP-tagged) - Mouse cortistatin (Cort) | 10 ug |
$450.00
|
|
Plasmid Map for MG215280
Download sequence for
Cloning scheme for MG215280
Circular map for MG215280
Citations
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