Cort (NM_007745) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG215280
Cort (tGFP-tagged) - Mouse cortistatin (Cort), (10ug)
  $489.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cort
Synonyms CS; CST; PC; PCST
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215280 representing NM_007745
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGGTGGCCGAGGCACAGGAGGCAAGTGGCCCTCAGCCTTCGGGCTGCTGCTGCTCTGGGGGGTCG
CAGCCTCCGCCCTTCCCCTGGAGAGTGGCCCTACTGGCCAGGACAGTGTGCAGGAAGCCACCGAGGGGAG
GAGCGGCCTTCTGACTTTCCTTGCCTGGTGGCACGAGTGGGCTTCCCAAGCCAGCTCCAGCACCCCCGTC
GGAGGGGGTACCCCCGGGCTGTCCAAGAGCCAGGAAAGGCCACCCCCCCAACAGCCCCCACACCTGGATA
AAAAGCCCTGCAAGAACTTCTTCTGGAAAACCTTCTCCTCGTGCAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215280 representing NM_007745
Red=Cloning site Green=Tags(s)

MMGGRGTGGKWPSAFGLLLLWGVAASALPLESGPTGQDSVQEATEGRSGLLTFLAWWHEWASQASSSTPV
GGGTPGLSKSQERPPPQQPPHLDKKPCKNFFWKTFSSCK

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007745
ORF Size 327 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007745.4, NP_031771.1
RefSeq Size 498 bp
RefSeq ORF 330 bp
Locus ID 12854
UniProt ID P56469
Cytogenetics 4 78.89 cM
Summary This gene encodes a member of the somatostatin family of multifunctional peptides attributed with neurohormone, neurotransmitter/modulator and autocrine/paracrine actions. The encoded preproprotein undergoes proteolytic processing to generate a mature functional peptide that can bind to somatostatin receptors. Mice lacking the encoded protein exhibit elevated levels of growth hormone in the plasma without major changes in somatic growth and have exacerbated nociceptive responses to neuropathic and inflammatory pain sensitization. Transgenic mice overexpressing the encoded protein in neurons do not express long-term potentiation in the dentate gyrus and exhibit deficits in synaptic plasticity and learning. provided by RefSeq, Nov 2015
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Documents
Resources
"NM_007745" in other vectors (4)
SKU Description Size Price
MC208307 Cort (untagged) - Mouse cortistatin (Cort), (10ug) 10 ug
$165.00
MR215280 Cort (Myc-DDK-tagged) - Mouse cortistatin (Cort) 10 ug
$289.00
MR215280L3 Lenti ORF clone of Cort (Myc-DDK-tagged) - Mouse cortistatin (Cort) 10 ug
$450.00
MR215280L4 Lenti ORF clone of Cort (mGFP-tagged) - Mouse cortistatin (Cort) 10 ug
$450.00

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