Cpb1 (NM_029706) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG215270
Cpb1 (tGFP-tagged) - Mouse carboxypeptidase B1 (tissue) (Cpb1), (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cpb1
Synonyms 0910001A18Rik; 1810063F02Rik; 2210008M23Rik; AI504870
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215270 representing NM_029706
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGCTGCTACTGGCCCTGGTGAGTGTGGCCCTGGCTCATGCTTCTGAGGAGAACTTTGATGGCAACC
GGGTGTTCCGTGTCAGTGTACATGATGAAGATGACGTCAGCTTAATTCAGGAACTAGCCAACACCAAACA
GATTGATTTCTGGAAACCAGATTCTGCTACACAAGTGAAACCTCTTACTACAGTTGACTTTCATGTTAAA
GCAGAAGATGTCGTTGATGTGGAGAACTTTCTCGAGGAGAATGAAGTTCTCTATGAGGTATTGATAAGCA
ACGTGAAACATATTCTGGAATCCCAGTTTGACAGCCACACCCGTGCAAGTGGACACAGCTACACCAAGTA
CAACAACTGGGAAACGATAGAGGCATGGATTCAACAAGTTGCCAATGATAATCCAGACCTTGTTTCTAAG
AGAGTCATTGGAACCACATTTGAAGGACGTAACATGTATGTTCTCAAGATTGGCAAAGATAGACCAAATA
AGCCTGCCTTCTTCATCGACTGTGGTTTCCACGCAAGAGAGTGGATTTCTCCTGCATTCTGTCAGTGGTT
TGTGAGAGAGGCTGTCCGTACCTATAAACAAGAGATCCACATGAAACGACTTCTTGACGAACTGGACTTT
TATGTTCTGCCTGTGGTCAACATTGACGGCTATGTCTACACATGGGCTAAGGACCGAATGTGGAGAAAAA
CCCGCTCTACTACGGCTGGAAGTTCCTGCTTTGGTGTAGACCCCAACAGAAATTTTGATGCTGGCTGGTG
TGAAGTGGGAGCTTCTCGGAGTCCCTGCTCTGACACTTACTGTGGACCAACTCCAGAGTCAGAAAAAGAG
ACAAAGGCCCTGGCAGATTTCATCCGCCAGAACCTCTCCTCCATCAAGGCCTATCTGACCGTCCACTCCT
ACTCACAGATGATGCTCTATCCTTACTCCTACGACTATAAGCTGCCCGAGAACTATGAGGAGCTGAATGC
CCTGGTGAAAGGTGCAGCAAAGGAGCTTTCCACTCTGCATGGCACCAAGTACACATATGGCCCAGGAGCT
ACAACGATCTATCCTGCTGCTGGGGGATCTGACGACTGGGCTTATGATCAGGGAATCAAATATTCCTTTA
CCTTTGAACTCCGGGATAAAGGCTTCTTTGGCTTTCTGCTTCCTGAGTCTCAGATCCGCCAAACCTGTGA
GGAGACAATGCTTGCAGTCAAGTACATTGCCAATTATGTCCTAGAGCATCCATAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215270 representing NM_029706
Red=Cloning site Green=Tags(s)

MLLLLALVSVALAHASEENFDGNRVFRVSVHDEDDVSLIQELANTKQIDFWKPDSATQVKPLTTVDFHVK
AEDVVDVENFLEENEVLYEVLISNVKHILESQFDSHTRASGHSYTKYNNWETIEAWIQQVANDNPDLVSK
RVIGTTFEGRNMYVLKIGKDRPNKPAFFIDCGFHAREWISPAFCQWFVREAVRTYKQEIHMKRLLDELDF
YVLPVVNIDGYVYTWAKDRMWRKTRSTTAGSSCFGVDPNRNFDAGWCEVGASRSPCSDTYCGPTPESEKE
TKALADFIRQNLSSIKAYLTVHSYSQMMLYPYSYDYKLPENYEELNALVKGAAKELSTLHGTKYTYGPGA
TTIYPAAGGSDDWAYDQGIKYSFTFELRDKGFFGFLLPESQIRQTCEETMLAVKYIANYVLEHPY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029706
ORF Size 1245 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029706.2, NP_083982.1
RefSeq Size 1494 bp
RefSeq ORF 1248 bp
Locus ID 76703
Cytogenetics 3 6.29 cM
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Documents
Resources
"NM_029706" in other vectors (4)
SKU Description Size Price
MC211767 Cpb1 (untagged) - Mouse carboxypeptidase B1 (tissue) (Cpb1), (10ug) 10 ug
$503.00
MR215270 Cpb1 (Myc-DDK-tagged) - Mouse carboxypeptidase B1 (tissue) (Cpb1) 10 ug
$289.00 $457.00
MR215270L3 Lenti ORF clone of Cpb1 (Myc-DDK-tagged) - Mouse carboxypeptidase B1 (tissue) (Cpb1) 10 ug
$757.00
MR215270L4 Lenti ORF clone of Cpb1 (mGFP-tagged) - Mouse carboxypeptidase B1 (tissue) (Cpb1) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.