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Kncn (NM_001039124) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215233
Kncn (tGFP-tagged) - Mouse kinocilin (Kncn), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kncn
Synonyms Kino; L5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215233 representing NM_001039124
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACATCCCCATCAGCACCAGAGACTTCCGCTGCCTGCAGCTGGCCTGTGTGGCCCTCGGCCTGGTGG
CCGGCAGCATCATCATCGGTGTCTCCGTGTCCAAGGCTGCTGCTGCTGTGGGTGGTATCTTCCTTGGTGC
TGCTGGGCTTGGGCTCCTCATCTTCGCCTACCCCTTCCTGAAGGCTCGGTTCAACCTGGACCACATCCTA
CCTGCCATAGGAAACCTGAGAATCCATCCTAACTCAGGACCAGACCATGGGGAGGGAAGATCTAGCAACA
ACAGCAATAAAGAAGGAGCCCGCAGCGGCCTGTCCACAGTGACCAGAACCCTGGAGAAGCTGAAGCCTGG
AGGCCGGGGAACTGAGGAGGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215233 representing NM_001039124
Red=Cloning site Green=Tags(s)

MDIPISTRDFRCLQLACVALGLVAGSIIIGVSVSKAAAAVGGIFLGAAGLGLLIFAYPFLKARFNLDHIL
PAIGNLRIHPNSGPDHGEGRSSNNSNKEGARSGLSTVTRTLEKLKPGGRGTEEG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039124
ORF Size 372 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039124.4, NP_001034213.1
RefSeq Size 951 bp
RefSeq ORF 375 bp
Locus ID 654462
UniProt ID Q307W7
Cytogenetics 4 D1
Summary May play a role in stabilizing dense microtubular networks or in vesicular trafficking.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001039124" in other vectors (4)
SKU Description Size Price
MC215340 Kncn (untagged) - Mouse kinocilin (Kncn), (10ug) 10 ug
$165.00
MR215233 Kncn (Myc-DDK-tagged) - Mouse kinocilin (Kncn) 10 ug
$289.00
MR215233L3 Lenti ORF clone of Kncn (Myc-DDK-tagged) - Mouse kinocilin (Kncn) 10 ug
$450.00
MR215233L4 Lenti ORF clone of Kncn (mGFP-tagged) - Mouse kinocilin (Kncn) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.