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Usp17lc (NM_010089) Mouse Tagged ORF Clone

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SKU
MG215208
Usp17lc (tGFP-tagged) - Mouse ubiquitin specific peptidase 17-like 5 (Usp17l5), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $707.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Usp17lc
Synonyms Dub-2; Dub2; Dub2b; Usp17l5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215208 representing NM_010089
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGTTTCTCTTTCCTTCCCAGAAGCAGATCCAGCCCTATCATCTCCTGGTGCCCAACAGCTGCATC
AGGATGAAGCTCAGGTAGTGGTGGAGCTAACTGCCAATGACAAGCCCAGTCTGAGTTGGGAATGTCCCCA
AGGACCAGGATGCGGGCTTCAGAACACAGGCAACAGCTGCTACCTGAATGCAGCCCTGCAGTGCTTGACA
CACACACCACCTCTAGCTGACTACATGCTGTCCCAGGAGTACAGTCAAACCTGTTGTTCCCCAGAAGGCT
GTAAGATGTGTGCTATGGAAGCCCATGTAACCCAGAGTCTCCTGCACTCTCACTCGGGGGATGTCATGAA
GCCCTCCCAGATTTTGACCTCTGCCTTCCACAAGCACCAGCAGGAAGATGCCCATGAGTTTCTCATGTTC
ACCTTGGAAACAATGCATGAATCCTGCCTTCAAGTGCACAGACAATCAGAACCCACCTCTGAGGACAGCT
CACCCATTCATGACATATTTGGAGGCTTGTGGAGGTCTCAGATCAAGTGTCTCCATTGCCAGGGTACCTC
AGATACATATGATCGCTTCCTGGATGTCCCCCTGGATATCAGCTCAGCTCAGAGTGTAAATCAAGCCTTG
TGGGATACAGAGAAGTCAGAAGAGCTACGTGGAGAGAATGCCTACTACTGTGGTAGGTGTAGACAGAAGA
TGCCAGCTTCCAAGACCCTGCATATTCATAGTGCCCCAAAGGTACTCCTGCTAGTGTTAAAGCGCTTCTC
GGCCTTCATGGGTAACAAGTTGGACAGAAAAGTAAGCTACCCAGAGTTCCTTGACCTGAAGCCATACCTG
TCCCAGCCTACTGGAGGACCTTTGCCTTATGCCCTCTATGCTGTCCTGGTCCATGAAGGTGCGACTTGTC
ACAGTGGACATTACTTCTCTTATGTCAAAGCCAGACATGGGGCATGGTACAAGATGGATGATACTAAGGT
CACCAGCTGCGATGTGACTTCTGTCCTGAATGAGAATGCCTATGTGCTCTTCTATGTGCAGCAGACTGAC
CTCAAACAGGTCAGTATTGACATGCCAGAGGGCAGAGTACATGAGGTTCTCGACCCTGAATACCAGCTGA
AGAAATCCCGGAGAAAAAAGCATAAGAAGAAAAGCCCTTGCACAGAAGATGCGGGAGAGCCCTGCAAAAA
CAGGGAGAAGAGAGCAACCAAAGAAACCTCCTTAGGGGAGGGGAAAGTGCTTCAGGAAAAGAACCACAAG
AAAGCTGGGCAGAAACATGAGAATACCAAACTTGTGCCTCAGGAACAGAACCACCAGAAACTTGGGCAGA
AACACAGGATCAATGAAATCTTGCCTCAGGAACAGAACCACCAGAAAGCTGGGCAGAGCCTCAGGAACAC
GGAAGGTGAACTTGATCTGCCTGCTGATGCAATTGTGATTCACCTGCTCAGATCCACAGAAAACTGGGGC
AGGGATGCTCCAGACAAGGAGAATCAACCCTGGCACAATGCTGACAGGCTCCTCACCTCTCAGGACCCTG
TGAACACTGGGCAGCTCTGTAGACAGGAAGGAAGACGAAGATCAAAGAAGGGGAAGAACAAGAACAAGCA
AGGGCAGAGGCTTCTGCTTGTTTGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215208 representing NM_010089
Red=Cloning site Green=Tags(s)

MVVSLSFPEADPALSSPGAQQLHQDEAQVVVELTANDKPSLSWECPQGPGCGLQNTGNSCYLNAALQCLT
HTPPLADYMLSQEYSQTCCSPEGCKMCAMEAHVTQSLLHSHSGDVMKPSQILTSAFHKHQQEDAHEFLMF
TLETMHESCLQVHRQSEPTSEDSSPIHDIFGGLWRSQIKCLHCQGTSDTYDRFLDVPLDISSAQSVNQAL
WDTEKSEELRGENAYYCGRCRQKMPASKTLHIHSAPKVLLLVLKRFSAFMGNKLDRKVSYPEFLDLKPYL
SQPTGGPLPYALYAVLVHEGATCHSGHYFSYVKARHGAWYKMDDTKVTSCDVTSVLNENAYVLFYVQQTD
LKQVSIDMPEGRVHEVLDPEYQLKKSRRKKHKKKSPCTEDAGEPCKNREKRATKETSLGEGKVLQEKNHK
KAGQKHENTKLVPQEQNHQKLGQKHRINEILPQEQNHQKAGQSLRNTEGELDLPADAIVIHLLRSTENWG
RDAPDKENQPWHNADRLLTSQDPVNTGQLCRQEGRRRSKKGKNKNKQGQRLLLVC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010089
ORF Size 1635 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010089.3, NP_034219.3
RefSeq Size 1697 bp
RefSeq ORF 1638 bp
Locus ID 13532
UniProt ID G5E8I7
Cytogenetics 7 54.85 cM
Summary Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes (PubMed:8995226, PubMed:11443643). Important for preimplantation stage embryonic development (PubMed:22984479).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010089" in other vectors (4)
SKU Description Size Price
MC218799 Usp17lc (untagged) - Mouse ubiquitin specific peptidase 17-like 5 (Usp17l5), (10ug) 10 ug
$559.00
MR215208 Usp17lc (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 17-like 5 (Usp17l5) 10 ug
$289.00 $507.00
MR215208L3 Lenti ORF clone of Usp17lc (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 17-like 5 (Usp17l5) 10 ug
$807.00
MR215208L4 Lenti ORF clone of Usp17lc (mGFP-tagged) - Mouse ubiquitin specific peptidase 17-like 5 (Usp17l5) 10 ug
$807.00

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