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Zbed5 (NM_183088) Mouse Tagged ORF Clone

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SKU
MG215197
2410018M08Rik (tGFP-tagged) - Mouse SCAN domain containing 3 (Scand3), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $892.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zbed5
Synonyms 2410018M08Rik; Scand3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215197 representing NM_183088
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCACGTGGAAGCCGAATCCGCACTTCCCGCGTGACCCTTCTGGCCGGCCGGGCCCCTCAGATGAGGG
CTGCTCCCCGAAGAGCACCTGCAGCTCAGCCTCCAGCAGCAGCTGCGCCATCTGCAGTTGGCTCACCTGC
CGCTGCGCCCCGGCAGCCAGGCCTGATGGCCCAGATGGCTACCACCGCGGCCGGTGTGGCTGTGGGCTCT
GCAGTGGGACACACCCTGGGTCACGCCATCACTGGGGGCTTCAGCGGAGGTGGCAGTGCTGAGCCCGCAA
AGCCCGACATCACTTACCAGGAGCCTCAGGGAGCCCAGCTGCAGAACCAGCAGTCTTTTGGACCTTGCTC
TCTAGAGATCAAGCAGTTTCTGGAGTGTGCTCAGAACCAGAGCGATGTCAAGCTCTGTGAGGGCTTCAAC
GAGGTGCTGCGGCAGTGCAGGATTGCAAATGTGGACCATCCCAGGCCAGTGGATCATGGAGAGAGGAACG
TTAAGAGAAGAAAATATAATGAGGGTTTTTTGCAGTATGGTTTTACCTCAACCATCACAGTAGGAATTGA
GAGACCACAGTGTGTCATTTGTGGGGTGGTTCTGTCGGCGGAGTCGATGAAACCCAACAAACTGAAACGC
CATTTTGAGAGCAAGCATTCCAGCTTCGCCGGCAAGGATACCAACTATTTCAGAAGCAAAGCTGATGGAC
TCAAGAAAGCCAGACCTGATACTGGCAGCAAGTCCAACAAACAGAACGTGGCAGCCGTGGAAACTTCCTA
TTTGGTGGCCCTCAGAATTGCCAGAGATATGAAACCGCACACCTTTGCAGAGCACTTACTGTTCCCGGTG
GCCAAGGACAGGGTTCGCGTCATGATCGGAGACGAATTTGTTACAAAACTGAGTGCAGTCTCCTTATCTA
ACGACACTGTCCGAAGAAGAATACATGACATGTCTGCTGATATTCTTGATCAGGTGGTCCAGGAAATTAA
ATCTGCTCCACTTCCAATATGCAGCATCCAGCTCGATGAATCGACAGACGTTGCAAACTGTTTACAGTTA
ATGGTTTACGTGAGGTATATTAATGATGGTGACTTTAAAGATGAGTTTCTTTGCTGCAAACCTCTTGAAA
GGACAGCTACTGCCCTTGATGTATTTGAGGCAGTCGACTCGTTTCTGAGACAGCATGAGATCTCTTGGAA
AAGCATCTGTGGAGTTTGCACAGACGGTGCCCCAGCTACGCTGGGCTGTCAATCTGGGTTTCAGCGCCTG
GTCCTAAATGAGTCCCCAAAAGCCATTGGAGCTCATTGTATGCTTCATCTGCAAACATTAGCAATGAAGA
CGCTGCCCCAAGACTTCCAGGAAGTAATGAAAAGTGTCCTGAGTTCTGTCAATTTCGTAAAGGCGAGTTC
TTTGAACAGTCGACTGTTTCTGCAGCTCTGCAGTGATTTAGATGAGCCAAGCAAAACCCTGCTACTTCAT
ACTGAAGGGAGATGGCTGTCAAGAGGGAAAGTTCTAAAACGTATCTTTGAGCTCCGTGACGAACTCAAAA
TGTTTTTTAACCAAAAAGCAATACGGCAGTTCGAAGCACTTTTCAGTGATAACAGTGCATTGCAGAAGGT
AGCTTATTTGGTCGATATCTTTACCATTTTGAATGAGTTGAATTTATCACTGCAAGGACCAAATTCAACC
TGCCTGGATCTGTCTGAAAAGATCCACTCATTCCAAATGAAACTTCAACTCTGGCAAAAAAAATTGGATG
AAAATAAATTTTACATGTTGCCTACCTTATCCGCCTTCTTTGAGGAACATGACATTGAACAGCACAAAAG
GATTACAGTGGTGATTTCCGTGAAAGAACACTTGGACATGCTTGCAAGTGAAATTTCCTGGTACTTTCCA
AATCTACCCGAGATTCCGTTTGCACTCGCCAGGAGCCCGTTCTCAGTCAAAGCTGAAGATGTTCCCGAGA
CTGCGCAAGAAGATTTCACCAGACTTACCAACAGCGACGCGGCGAGAGCTGATTTCTCCACAATGCCAGT
CACACAGTTCTGGGTCAAGTGTTTGCAGTCATACCCAGTTCTGTCAGAGATGGTGTTGCGCCTCCTCCTT
CCTTTTCCAACAACATATCTGTGTGAAACAGGGTTCTCAAGTTTGTTAGTTATCAAGTCTAAATACAGAA
GTAGACTTGTGGTGGAAGATGATCTTCGCTGTGCTCTTGCAAAGACCACCCCGAGAATTTCTGATCTGGT
GAGAAAGAAGCAGTCTCAACCTTCGCAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215197 representing NM_183088
Red=Cloning site Green=Tags(s)

MPRGSRIRTSRVTLLAGRAPQMRAAPRRAPAAQPPAAAAPSAVGSPAAAPRQPGLMAQMATTAAGVAVGS
AVGHTLGHAITGGFSGGGSAEPAKPDITYQEPQGAQLQNQQSFGPCSLEIKQFLECAQNQSDVKLCEGFN
EVLRQCRIANVDHPRPVDHGERNVKRRKYNEGFLQYGFTSTITVGIERPQCVICGVVLSAESMKPNKLKR
HFESKHSSFAGKDTNYFRSKADGLKKARPDTGSKSNKQNVAAVETSYLVALRIARDMKPHTFAEHLLFPV
AKDRVRVMIGDEFVTKLSAVSLSNDTVRRRIHDMSADILDQVVQEIKSAPLPICSIQLDESTDVANCLQL
MVYVRYINDGDFKDEFLCCKPLERTATALDVFEAVDSFLRQHEISWKSICGVCTDGAPATLGCQSGFQRL
VLNESPKAIGAHCMLHLQTLAMKTLPQDFQEVMKSVLSSVNFVKASSLNSRLFLQLCSDLDEPSKTLLLH
TEGRWLSRGKVLKRIFELRDELKMFFNQKAIRQFEALFSDNSALQKVAYLVDIFTILNELNLSLQGPNST
CLDLSEKIHSFQMKLQLWQKKLDENKFYMLPTLSAFFEEHDIEQHKRITVVISVKEHLDMLASEISWYFP
NLPEIPFALARSPFSVKAEDVPETAQEDFTRLTNSDAARADFSTMPVTQFWVKCLQSYPVLSEMVLRLLL
PFPTTYLCETGFSSLLVIKSKYRSRLVVEDDLRCALAKTTPRISDLVRKKQSQPSH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183088
ORF Size 2268 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183088.2, NP_898911.2
RefSeq Size 2492 bp
RefSeq ORF 2271 bp
Locus ID 71970
Cytogenetics 5 G1.3
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Documents
Resources
"NM_183088" in other vectors (4)
SKU Description Size Price
MC221385 2410018M08Rik (untagged) - Mouse RIKEN cDNA 2410018M08 gene (2410018M08Rik), (10ug) 10 ug
$762.00
MR215197 2410018M08Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2410018M08 gene (2410018M08Rik) 10 ug
$589.00 $692.00
MR215197L3 Lenti ORF clone of 2410018M08Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2410018M08 gene (2410018M08Rik) 10 ug
$992.00
MR215197L4 Lenti ORF clone of 2410018M08Rik (mGFP-tagged) - Mouse RIKEN cDNA 2410018M08 gene (2410018M08Rik) 10 ug
$992.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.