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Nlrp1b (NM_001040696) Mouse Tagged ORF Clone

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SKU
MG215179
Nlrp1b (tGFP-tagged) - Mouse NLR family pyrin domain containing 1B (Nlrp1b) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,222.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nlrp1b
Synonyms ENSMUSG00000070390; Nalp1b; OTTMUSG00000006087; OTTMUSG00000006089
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215179 representing NM_001040696
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACAATCTCAGCCCAAGAAGAAAAGTCGCACAAAGGTGGCTCAGCATGAGGGTCAACTAAATTTGA
ACCCCACCTTCAAAACAAGGAAACGTAAAGAGGTGGAGCTGATGAAGCGCAGACCCAAGCCAGAGGGACA
CTTGAAGCTAGGGACAATTCCAAAAGTACACATAAAACAAAAAGGAGAGACTCTGGACCCCACATGGTCA
AGGAAAAGAAAGAATCTGGTCCAAAAGTTAACAAACCTACTACTCTTTGAAAAATTGTGCTCAAGAGGCT
CAGAAAACTTGATCAGGAAAAGCTGGTATTCATGTGAAGAAGAAGAAAGAGGACATATGATTGAGATCCA
AGACTTATTTGGCCCAAACAGAGGTACCCATAAAAAGCCTCAATTAGTCATAATAGAGGGTGCTGCTGGA
ATTGGGAAGTCAACACTGGCCAGGCAGGTGAAGAGAGCCTGGAAGGAAGGCCAGCTCTACAGGAATCACT
TCCAGCATGTCTTCTTCCTCAGTTGCAGGGAGCTGGCCCAGTATGAGCAGCTGAGTCTGGCTGAGCTCAT
AGCACAAGGCCAGGAAGTGCCCACCGTTCCCATCAGGCAGATCCTGTCTCACCCTAAGGAGCTGCTCTTC
ATCCTGGATGGCATAGATGAGCCAGCATGGGTCTTGGCAGACCAGAATCCTGAGCTATGTCTGCACTGGA
GTCAAAGACAGCCTGTGCACACTCTGCTGGGCAGTTTACTAGGGAAATCCATCCTTCCTGGGGCTTCCTT
CTTGCTCACAGCTCGGACCACAGCTCTACAGAAGATCATTCCTTATGTGGGGCAGCCACGTCGGGTGGAA
GTCCTGGGGTTCTCCAAGTTTGAACGGGAGGTCTATTTCCGCAAATATTTTGTGAAGGAGAGCGATGCAA
TTGCTGCTTTTAGATTGGTGGTATCTAACCCAGTGCTCCTGACCCTGTGTGAGGTTCCCTGGGTGTGCTG
GTTGGTCTGCACTTGCCTGAAAAAGCAGATGGAGCAGGGTGGAGAGCTCTCCCTGACCTCACAGACCACC
ACAGCCCTCTGCCTGAAATACCTTTCCCTGACAATCCCAGGGCAGCACATGAGGACCCAGCTCAGAGCCC
TCTGCTCACTGGCTGCTGAGGGGATCTGCCAAAGAAGAACCCTGTTCAGTGAAAGCGACCTCTGTAAGCA
AGGGTTAGCAGAGGATGCCATTGCCACTTTCCTGAAGATCGGTATCCTTCAAAAGCAGGCCAGCTCTCTG
AGCTACAGCTTTGCCCACTTGTGTCTCCAGGAGTTCTTTGCAGTCATGTCTTACATCTTGGAGGATAGTG
ATGAAAGATGTGATGGTATGGAATTCAAAAGAACTGTGGAAACACTGATAGAAGTATATGGAAGACATAC
TCTGTGTGAGGAACCCACTGTGCACTTTCTATTTGGTCTTGTGAATGAACAGGGAATGAGAGAAATGAAA
AAGATCTTTGACTGCAAACTGCCTTTGGGGACAGAGTTGAAGATGCTAAAGAGCACCCTAGGGAACCCAA
CCTATCAACATCATCTGGGTTTGCTCCACTGTCTGTATGAAAGTCAGGAAGAGGTGCTCCTGACATATGT
GTTATGTAATCTTCACTTAACAGGGCCTGACAAAAATTACATGGAAGCCACAGTGTCACAGACAAATGTG
AAGCACCTGGTGATACAGACAGATATGGAGCTCATGGTGGTCACTTTCTGCATTCAGTTCTGTTGTCACG
TGAGGAGTCTTCGGGTGAACATGAAAGGACAGCAGGGACATAAGCTGACAGTTGCCAGTATGGTTCTGTA
CAGGTGGACCCCAATCACTAATGCCAGTTGGAAGATTCTTTTCTACAATCTTAAATTCAACAGTAATCTG
GAGGGGTTGGACCTCAGTGGAAATCCACTGAGTTACTCTGCAGTGCAATATCTCTGTGATGCCATGATAT
ACCCTGGCTGCCAACTAAAGACTTTGTGGCTTGTTGAATGCGGCCTCACACCCACATACTGCTCACTCCT
GGCCTCAGTGCTCAGTGCCTGCTCCAGCCTGAGAGAGCTAGACCTGCAGCTAAATGACCTGTGTGACGAT
GGTGTAAGGATGCTGTGTGAGGGGCTCAGGAATCGTGCCTGCAACCTCAGAATCCTGCGGCTGGACCTGT
ACTCTCTCAGTGCCCAGGTGATTACAGAGCTCAGGACACTGGAGGAAAATAATCTGAAGCTGCACATCTC
CAGCATCTGGATGCCACAAATGATGGTCCCCACTGAGAACATGGATGAAGAAGATATCCTGACCTCATTC
AAGCAGCAGAGACAACAGTCAGGAGCCAATCCCATGGAAATTCTGGGGACTGAAGAAGACTTCTGGGGCC
CTATAGGACCTGTGGCTACTGAGGTGGTTTACAGAGAAAGGAACCTGTACCGAGTTCAATTGCCCATGGC
TGGTTCCTACCACTGTCCCAGCACAAGACTCCACTTTGTAGTGACAAGGGCAGTGACAATAGAGATCGAA
TTCTGTGCCTGGAGCCAATTCCTGGACAAGACTCCCTTGCAGCAGAGTCACATGGTGGTTGGACCTCTGT
TTGACATCAAGGCTGAGCAAGGAGCTGTGACTGCAGTGTACCTCCCTCATTTTGTGTCCCTCAAAGACAC
AAAGGCAAGCACATTTGACTTCAAGGTGGCCCACTTTCAAGAACATGGGATGGTTCTAGAAACGCCAGAT
AGGGTGAAGCCTGGATACACAGTACTGAAAAACCCAAGCTTCTCCCCAATGGGAGTTGTACTGAGAATAA
TCCCTGCTGCCCGGCACTTCATCCCCATTACTTCCATCACACTGATCTACTATCGAGTCAATCAGGAAGA
AGTCACCCTTCACCTCTACCTAGTCCCTAATGACTGCACCATACAGAAGGCCATAGATGATGAAGAAATG
AAGTTTCAGTTTGTGAGAATAAACAAGCCACCCCCAGTAGACAATCTTTTCATTGGCTCCCGCTACATTG
TGTCTGGTTCTGAGAACCTGGAAATCACCCCAAAGGAACTGGAGCTGTGCTACAGGAGCAGCAAGGAATT
CCAACTCTTCTCTGAGATATACGTTGGAAACATGGGTTCAGAGATTAAGCTGCAAATCAAAAACAAAAAG
CACATGAAACTCATATGGGAAGCCTTGCTGAAACCAGGAGACCTCAGACCTGCTCTGCCCAGGATTGCCC
AAGCCCTCAAAGATGCCCCTTCCTTGCTGCACTTCATGGACCAGCATCGGGAGCAGCTGGTAGCCCGAGT
GACATCAGTGGACCCTCTCTTGGACAAGCTGCATGGTCTGGTGCTGAATGAAGAATCCTATGAGGCAGTG
CGGGCTGAGAACACAAACCAAGATAAGATGAGGAAGCTCTTCAACCTCAGCCGGTCCTGGAGTCGAGCCT
GCAAAGACCTATTCTACCAAGCTCTGAAGGAGACCCATCCTCACCTGGTCATGGACCTCTTAGAAAAGTC
AGGTGGGGTCTCTTTGGGATCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215179 representing NM_001040696
Red=Cloning site Green=Tags(s)

MEQSQPKKKSRTKVAQHEGQLNLNPTFKTRKRKEVELMKRRPKPEGHLKLGTIPKVHIKQKGETLDPTWS
RKRKNLVQKLTNLLLFEKLCSRGSENLIRKSWYSCEEEERGHMIEIQDLFGPNRGTHKKPQLVIIEGAAG
IGKSTLARQVKRAWKEGQLYRNHFQHVFFLSCRELAQYEQLSLAELIAQGQEVPTVPIRQILSHPKELLF
ILDGIDEPAWVLADQNPELCLHWSQRQPVHTLLGSLLGKSILPGASFLLTARTTALQKIIPYVGQPRRVE
VLGFSKFEREVYFRKYFVKESDAIAAFRLVVSNPVLLTLCEVPWVCWLVCTCLKKQMEQGGELSLTSQTT
TALCLKYLSLTIPGQHMRTQLRALCSLAAEGICQRRTLFSESDLCKQGLAEDAIATFLKIGILQKQASSL
SYSFAHLCLQEFFAVMSYILEDSDERCDGMEFKRTVETLIEVYGRHTLCEEPTVHFLFGLVNEQGMREMK
KIFDCKLPLGTELKMLKSTLGNPTYQHHLGLLHCLYESQEEVLLTYVLCNLHLTGPDKNYMEATVSQTNV
KHLVIQTDMELMVVTFCIQFCCHVRSLRVNMKGQQGHKLTVASMVLYRWTPITNASWKILFYNLKFNSNL
EGLDLSGNPLSYSAVQYLCDAMIYPGCQLKTLWLVECGLTPTYCSLLASVLSACSSLRELDLQLNDLCDD
GVRMLCEGLRNRACNLRILRLDLYSLSAQVITELRTLEENNLKLHISSIWMPQMMVPTENMDEEDILTSF
KQQRQQSGANPMEILGTEEDFWGPIGPVATEVVYRERNLYRVQLPMAGSYHCPSTRLHFVVTRAVTIEIE
FCAWSQFLDKTPLQQSHMVVGPLFDIKAEQGAVTAVYLPHFVSLKDTKASTFDFKVAHFQEHGMVLETPD
RVKPGYTVLKNPSFSPMGVVLRIIPAARHFIPITSITLIYYRVNQEEVTLHLYLVPNDCTIQKAIDDEEM
KFQFVRINKPPPVDNLFIGSRYIVSGSENLEITPKELELCYRSSKEFQLFSEIYVGNMGSEIKLQIKNKK
HMKLIWEALLKPGDLRPALPRIAQALKDAPSLLHFMDQHREQLVARVTSVDPLLDKLHGLVLNEESYEAV
RAENTNQDKMRKLFNLSRSWSRACKDLFYQALKETHPHLVMDLLEKSGGVSLGS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001040696
ORF Size 3522 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001040696.1, NP_001035786.1
RefSeq Size 4159 bp
RefSeq ORF 3525 bp
Locus ID 637515
UniProt ID A1Z198
Cytogenetics 11 B4
Summary As the sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of Nlrp1b, CASP1, and possibly PYCARD. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP1 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (By similarity). May be activated by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, in a NOD2-dependent manner (PubMed:18511561). Might be activated by Toxoplasma gondii, although at a lower extent than allele 1 (PubMed:24218483). Contrary to Nlrp1b allele 1, allele 2 is not activated by Bacillus anthracis lethal toxin (PubMed:16429160, PubMed:21170303, PubMed:24492532).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001040696" in other vectors (4)
SKU Description Size Price
MC223845 Nlrp1b (untagged) - Mouse NLR family, pyrin domain containing 1B (Nlrp1b), transcript variant 1, (10ug) 10 ug
$1,125.00
MR215179 Nlrp1b (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 1B (Nlrp1b), transcript variant 1 10 ug
$589.00 $1,022.00
MR215179L3 Lenti ORF clone of Nlrp1b (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 1B (Nlrp1b), transcript variant 1 10 ug
$1,322.00
MR215179L4 Lenti ORF clone of Nlrp1b (mGFP-tagged) - Mouse NLR family, pyrin domain containing 1B (Nlrp1b), transcript variant 1 10 ug
$1,322.00

file-alt Citations

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