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Gm12250 (NM_001135115) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215178
Gm12250 (tGFP-tagged) - Mouse predicted gene 12250 (Gm12250), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gm12250
Synonyms Irgb10; OTTMUSG00000005723
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215178 representing NM_001135115
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTCAGTCTTCTTCTAAACCCGATGCAAAGGCTCACAATATGGCCTCCAGCCTTACTGAATTCTTCA
AGAATTTCAAGATGGAAAGCAAAATCATTTCTAAGGAGACCATCGATTCAATTCAGTCATGTATCCAAGA
AGGAGACATCCAGAAGGTGATTTCTATAATCAATGCTGCCTTGACAGACATTGAGAAAGCCCCCCTGAAC
ATCGCTGTGACAGGGGAGACAGGGGCAGGGAAGTCCACGTTCATTAATGCCCTTCGGGGAATAGGACATG
AGGAAAGTGAGTCAGCTGAGAGTGGAGCGGTGGAGACAACCAAGGACAGGAAGAAATACACCCACCCTAA
GTTCCCTAATGTGACCATCTGGGATCTCCCTGGGGTTGGGACAACTAACTTCAAACCAGAAGAATATCTG
AAGAAAATGAAGTTCCAGGAGTATGATTTTTTCCTGATCATCTCATCTGCTCGCTTTAGAGATAATGAAG
CCCAGCTGGCTGAAGCAATCAAAAAAATGAAAAAGAAGTTCTACTTTGTTCGGACCAAAATTGACAGTGA
TTTGTGGAATGAGAAGAAAGCTAAACCCAGTTCCTACAATAGGGAGAAAATCCTGGAGGTGATCCGAAGT
GACTGTGTAAAGAATCTGCAGAATGCTAACGCAGCCTCTACTCGTGTCTTCTTAGTCTCAAGCTTTGAGG
TAGCACAGTTTGATTTTCCTAGCCTGGAGTCCACCCTTTTGGAAGAGCTGCCAGCACACAAGCGCCACAT
CTTCGTGCAGTGCCTCCCTACCATTACTGAGCCTGCTATTGATCGCAGGAGAGATGTCCTGAAACAGACA
ATCTGGCTGGAGGCTCTGAAGGCTGGAGCGTCGGCCACCATCCCCATGATGTCATTCTTCAATGACGACA
TTGAGGAATTTGAGAAGATCCTGAGCCACTACAGGGCTTGCTTTGGGCTAGATGATGAGTCGCTGGAAAA
CATGGCCAAGGAGTGGTCCATGTCTGTGGAGGAGCTGGAGTCCACCATTAAGTCACCCCATTTGCTGTCA
AGTGAGCCGAATGAATCTGTGGCGGACAAGCTGGTGAAAACCATGGAGAAAATTTTTGCCGTCACTGGCG
GATTCGTAGCCACTGGCCTTTACTTTAGAAAGAGTTACTATATGCAAAATTATTTTCTTGACACAGTGAC
AGAGGATGCTAAAGTTCTACTTAAGAAAAAAGTCTTTTTGCAGGACAGTGTGGACTCTGAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215178 representing NM_001135115
Red=Cloning site Green=Tags(s)

MGQSSSKPDAKAHNMASSLTEFFKNFKMESKIISKETIDSIQSCIQEGDIQKVISIINAALTDIEKAPLN
IAVTGETGAGKSTFINALRGIGHEESESAESGAVETTKDRKKYTHPKFPNVTIWDLPGVGTTNFKPEEYL
KKMKFQEYDFFLIISSARFRDNEAQLAEAIKKMKKKFYFVRTKIDSDLWNEKKAKPSSYNREKILEVIRS
DCVKNLQNANAASTRVFLVSSFEVAQFDFPSLESTLLEELPAHKRHIFVQCLPTITEPAIDRRRDVLKQT
IWLEALKAGASATIPMMSFFNDDIEEFEKILSHYRACFGLDDESLENMAKEWSMSVEELESTIKSPHLLS
SEPNESVADKLVKTMEKIFAVTGGFVATGLYFRKSYYMQNYFLDTVTEDAKVLLKKKVFLQDSVDSE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001135115
ORF Size 1251 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001135115.1, NP_001128587.1
RefSeq Size 2702 bp
RefSeq ORF 1254 bp
Locus ID 631323
Cytogenetics 11 B1.3
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Documents
Resources
"NM_001135115" in other vectors (4)
SKU Description Size Price
MC215303 Gm12250 (untagged) - Mouse predicted gene 12250 (Gm12250), (10ug) 10 ug
$503.00
MR215178 Gm12250 (Myc-DDK-tagged) - Mouse predicted gene 12250 (Gm12250) 10 ug
$289.00 $457.00
MR215178L3 Lenti ORF clone of Gm12250 (Myc-DDK-tagged) - Mouse predicted gene 12250 (Gm12250) 10 ug
$757.00
MR215178L4 Lenti ORF clone of Gm12250 (mGFP-tagged) - Mouse predicted gene 12250 (Gm12250) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.