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Sbsn (NM_172205) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215160
Sbsn (tGFP-tagged) - Mouse suprabasin (Sbsn) transcript variant 1, (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $825.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sbsn
Synonyms 1110005D19Rik; AI747508
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215160 representing NM_172205, codon optimized.
Due to the complexity of NM_172205, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACCTGGTGTCCCTGCTCTCTTCATGCTGCCTGCTCGTGCTGCTCGGTACCCTGCCGGCACGAGCCG
CTCACGAGGACCCCGTGGAGAAAGTGATCGAAGGCTTCTCACGGGGACTGTCTAATGCCGAGCGAGAGGT
TGGAAAGGCTTTGGAGGGTATCAACAACGGCATAACACAGGCCGGTCGAGAAGTCGAAAAGATTTTCGGG
GAGCTGTCTAATATGGGCTCTCAGGCCGGAAAGAATGTGGAGCACGGTCTGGATAAGGTCGCACATGATA
TTAATTCCGGGATTGGTCACGCTGGGAAAGAGGCGGAGAAGTTTGCTCACGGGGTGAACCATGCTGCCGG
CCAGGTGGGTAAGGAAACTAATAAGATCATCCACCACGGAGTCAGTCAGGGTGGCAGCGAGGCTGGGAAA
TTCGGCCAGGGGAGCCACCACGCATTCGGGCAAGGAGGCAATGTGGCTGATAAGCTTGGCCACGAGACAC
ACCACGCCTTCGGGCAGGGCGGTAATGTTGCAGACAAACTGGGTCACGAAACACATCACGCCTTCGGACA
GGGCGGGAATGTGGCAGATAAATTGGGACACGGGGGGAACGTAGCAGACAAGTTGGGACACGGGACCCAT
CATGCCTTTGGACAGGGGGGGAACGTAGCTGATAAACTGGGGCATGAAACTCATCATGCCTTTGGCCAAG
GTGGCAATGTGGCTGACAAGCTTGGACATGGTACACACCACGCCTTCGGCCAGGGAGGAAATGTTGCAGA
CAAGTTGGGTCACGAGACTCATCATGCTTTCGGTCAGGGCGGGAATGTCGCCGAAAAGTTGGGGCATGAG
ACTCACCACGCTTTTGGTCAAGGGGGAAATATGGCTGATAAGTTTGGGCAGGGTGCACATCACGCTTTCG
GCCAGGGGGGAAACATGGCTGATAAATTCGGCCAAGGTGCTCACCACGCATTCGGTCAGGGCGGAAATAT
GGCTGACAAATTTGGTCAGGGTGCCCACCATGCGTTCGGCCAAGGAGGCAATATGGCAGATAAGTTCGGC
CAGGGCGCCCACCACGCCTTCGGACAAGGTGGAAACATGGCTGACAAGTTCGGACAAGGCGCTCATCATG
CGTTCGGACAGGGGAGAGACATGGCTGAAACATTTGACCAGGGCGCGCACCACGCCTTCGGACAGGGAGG
AAGGGAAGGCGGGAGGCTCGTGCAGGGGGCCGGACAGGGCCTGTCTCATGCTGCTAAGGAGGCTCAGCAA
TTCGGACATGGGCACGGCCACGGTTACTATGCAGCCGGCCAGACCTGGCAGGAAGGCGACAAGGTGATTA
GGCCAGGGGTGTCACAGGCAGGTGAGGAAATGGAACAGTTCGGGCAGGGTGTGCGCCACACCATAAAGCA
AGCCGAGAAGGAAGCCGAAAAAGTCGCACACGGGGTCCAGAACGGCGTCAACCAGGCGCAGAAGGAGGCC
GAAAAGGTGGCACACGGAGTACAGAATGGCGTGAATCAGGCCCAAAAGGAGGCGGAGAAGGTCGCTCATG
GGGTGCAAAATGGTGTGAATCAGGCCCAGAAGGAGGCAGAGAAGGTCGCTCACGGAGTGCAAACTGGAGT
AAATCAGGCAGGTAAAGAAACCCAGAGAGTTGGACAGGGCGTCCAAACTGGCTTCAATCAGGGCCAGAAG
GAGGCAGAAAAGGTCGCCCATGGAGTGCAGACGGGAGTGAACCAGGCGGGCAAAGAGACGCAGAAGGCTG
GCCAAGGCGTCAACTACGCGGCTGGGCAGGCTGAAAAGGAAGCCGAAAAGCTGGGGCAAGGGGTGCACCA
CGCGGCAGGGCAGGAAATGAATCGGTTGCAGCAGGATGTCCATAACGGCGTAAACCAGCCGTCAAAGGAA
GCTAACCAGCTTCTGAATGGTTCCCATCAAGGTCAGGGAGGATATGGGGGGCAGCACGGCGGCGCTGCCA
CCACTACAGTTGTGTCTGGCGCTAGTGTTAATAAGCCCTTCATCAATTTCCCCGCGCTTTGGCGGTCCAT
TGCCGCCACAATGCCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215160 representing NM_172205
Red=Cloning site Green=Tags(s)

MYLVSLLSSCCLLVLLGTLPARAAHEDPVEKVIEGFSRGLSNAEREVGKALEGINNGITQAGREVEKIFG
ELSNMGSQAGKNVEHGLDKVAHDINSGIGHAGKEAEKFAHGVNHAAGQVGKETNKIIHHGVSQGGSEAGK
FGQGSHHAFGQGGNVADKLGHETHHAFGQGGNVADKLGHETHHAFGQGGNVADKLGHGGNVADKLGHGTH
HAFGQGGNVADKLGHETHHAFGQGGNVADKLGHGTHHAFGQGGNVADKLGHETHHAFGQGGNVAEKLGHE
THHAFGQGGNMADKFGQGAHHAFGQGGNMADKFGQGAHHAFGQGGNMADKFGQGAHHAFGQGGNMADKFG
QGAHHAFGQGGNMADKFGQGAHHAFGQGRDMAETFDQGAHHAFGQGGREGGRLVQGAGQGLSHAAKEAQQ
FGHGHGHGYYAAGQTWQEGDKVIRPGVSQAGEEMEQFGQGVRHTIKQAEKEAEKVAHGVQNGVNQAQKEA
EKVAHGVQNGVNQAQKEAEKVAHGVQNGVNQAQKEAEKVAHGVQTGVNQAGKETQRVGQGVQTGFNQGQK
EAEKVAHGVQTGVNQAGKETQKAGQGVNYAAGQAEKEAEKLGQGVHHAAGQEMNRLQQDVHNGVNQPSKE
ANQLLNGSHQGQGGYGGQHGGAATTTVVSGASVNKPFINFPALWRSIAATMP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172205
ORF Size 2046 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172205.3, NP_757342.2
RefSeq Size 2310 bp
RefSeq ORF 2049 bp
Locus ID 282619
Cytogenetics 7
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Documents
Resources
"NM_172205" in other vectors (2)
SKU Description Size Price
MC220473 Sbsn (untagged) - Mouse suprabasin (Sbsn), transcript variant 1, (10ug) 10 ug
$688.00
MR215160 Sbsn (Myc-DDK-tagged) - Mouse suprabasin (Sbsn), transcript variant 1. Note: ORF is codon optimized 10 ug
$589.00 $625.00

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