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Nlrp2 (NM_177690) Mouse Tagged ORF Clone

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SKU
MG215150
Nlrp2 (tGFP-tagged) - Mouse NLR family pyrin domain containing 2 (Nlrp2), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,111.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nlrp2
Synonyms E330007A02Rik; Nalp2; NBS1; PAN1; PYPAF2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215150 representing NM_177690
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACATTTTGATCCCCTGGGTTTCAACCTAAAAGACGTCCTGAGAAAGCTGGACAAGACTGAGTTGA
ACAATTTCAAGAGAACATTGAGGAGCTGTTCTCTGCCAGATACAATGAAACAGATCAACAAGCTGACAAT
GGACCTGGCTAATGGAGCTCAGCTAGCAGAAATTCTCACAGACCACTGCCCCAGTGCTTGGATAAAGAGA
GTCACCGTCCAGATCTTAGAGGAAATAAACCGTGTAGATCTAGCTGAGTTAGTAGTAAAGCAAATCGAAG
AAGCAGTATTAAAGGTACCTGAGGAAAAGGTTTCTTCTAAACCAAGAGAACCCTCGGGGACACTAACTTT
CCCATGGAATTTTGTTCAAGGAGCTAAAAGGCCAGAGGACAAGCAAAAAGAGGAATGGAAGACTAGATAC
ACAGCTAAATGGAAGCAGAACTTCTGGCCTAAATGCAATAAAGAAATCTATGTGGTTACCGAGAGCTACA
AGACACTCCTGGCATTGTGTAACCCAAAGATAGAAACCCCATTTGCTCATGCAATTGTGCTGCATGGCCC
TCCAGGCTCTGGGAAAACCACAATGGCAAAGCAGCTAATGTTAGAATGGTCAGAGAGTAAGCAGGCCCAG
ATTTTCTCATGTGCCTTCTACATCAGCTGCAGGGAAGTAAATAATACTAAACCTTGCACTTTTGCCCACC
TCCTCTCCATGGACAATCCCTCCTGGAGGGACTGTGTGATTCGAGATCTAATCCTGGGAAAGGAATTCTT
ATTTGTGGTAGATGGTTTTGATGAGCTGACATTCCCAGCAGGAGCTCTGATCCGTGACCTTTGTGGTGAC
TGGAACACAGTGAAGCCTGTAGAGGTCTTACTGGGCAGTTTGCTGAAGAGGAAGATGGCACCTCATGCCA
CCCTGCTGGTAACCACACGGACACAGTCTTTGCACCAAATCTTTGTCATGATGGATCAGCCACTACTAGT
AGAAACTCTGGGCTTCTTGGAGCAAGAAAAGCAGGAGTACTTTCAGAAATACTTTGAAGATGAGGAGGGT
GAGGAGGAGGATAAAGGTGAGGGAAAGGCACTTAGAGCTCTCAAGGAAGTGAGATGCAATGCTGATCTAT
ACCAAATGGCCTCACTTCCTACTGCATGTGGGATATTCTGCCTCTGTCTGGAGCTAAGGATGAAGAAGGG
GGAAGACCTGTCTCTGACATGTCAGACATACACCTCTATGTTCCTGAACTTCCTGTGCGAAGTCTTCTCA
TCAGAAACCTGTGAGGACCATCTCAATGAGGAATTCCAAATCTTATTCAAGAAAATATGTATCCTGGCTG
CCAATAGTCTCTTGGAACAGGTGCCCATACTCTGTGAAGAAGACTTCTTGACATTAAAATTGAACCTAAA
CAATTTGCATCCCATGGTGTGCAGGCACATCCTCTTTAAGGACAGTTCCAGTACACACTGTCTCTCTTTC
ATCTGCCTCGGTATCCAGCAACTCCTGGCTGCAATAATATTTGTTCAGGAGCTTGGACAGGAAAGCAAGG
GTGTCTCTAAATATAGTATACAGAACATGCTTTCAAGGGAAGCAAGATTAAAAAACCCTGACCTGTCTGG
GCTGCTGCCATTTGTATTTGGCCTCCTGAATGAGACTCGTATTCAAGAGTTGAAGACTACTTTTGGCTGC
CAAATATCAACTGAGGTCAAGAGAAAATTCCTGGAGTGTGAGTCAGGGGAAAATAAACCCTTACTATTAC
TGATGAATATGCAAGAAATCTTGTCCTGTCTCTATGAATCTCAGGAGGAGGGGTTTGTGAAGGAAGCCAT
GGTCCTCTTTGAGGACATCTCCTTGCATTTGAAAACCAGCACTGACCTCATTCATGCTTCATTCTGCCTC
AAGAACTCTCAGAACTTGCAGACAATGTCCCTGAAAGTAGAAAAGGCGGTCTTTCCAGAGAATGTTGCTG
CACTAGAGTCCACAGCTAAGCATCAAAGGTCCCCGGATGAACAACGCATGTTAACTTTCTGGACAGACTT
TTGTGACACATTTAATTCAAATAAGAAACTGGTATTTCTAGACATCCATGAAAGCTTCCTCAACAGCTCT
GCACTGGAGATTCTTTGTGAAAAGCTGCCCTCTGCTTCCTGTTGTCTCCAGAAAGTGGTCCTCAAAAATA
TTTCCCCAGATGATGCCTACGAGAAATTGTGCCTCATTTTTAATGGTTATAAAACCATCTCACATCTGAT
CCTGCAAGGTGGCAACCTGGACAGCATGCATCACTCACTGTGTGAGGTTCTGAAAAATCCAGCCTGCAAC
CTGAAGTTTCTCAGCCTGGGATCTTGTTCTACTGCTGCTCAGAAATGGGATGACTTCTTCCCAGTCCTGA
AAGTCAATCAATCCCTGATATTTCTGGATCTCACAGACAACAGTCTCTTGGACAAAAGTGCTAAGCTCCT
ATGTAATATTTGGAAGGAACCAAAGTGCATACTGCAGAGGGTATCGTTGGAGAACTGTCAGCTCACTGAA
GCCTGCTGCAAGGACCTCTCTTCCGTTTTGATGGTCAGCCGAACTCTGACACACCTCAGCCTGGCCAACA
ATAAACTTGGGGATAACGGAGTGAAAAACCTGTGTGAAAGCATAAGCTGCACGGAGTGCAATCTACAGAC
CCTGGTGCTGTGGTCCTGTAACATCACCAACGCTGGTTGCCACTATCTCTCAAAGATGCTCAAACAAACA
CTGAGCCTGAAACACTTGGATCTGGGGCTAAATCGCATCGGAACCAAAGGAGCAAAGTTTCTGTGTGAGG
CTTTGAAGAACCCCAAGAGTAAATTGAAAAGTCTGTGGCTGTGTGGATGCTCCATCACTCCACTGAACTG
CCAAGACTTCTCTGAGACTCTTAGAAGCAACAAGAGCCTGAACACTCTAGACCTGTCTCAGAACGTCCTG
GGAACTGATGCCATAAAGACATTCTGTGAAGCCTTGAAACTTCAGATTTGCCCCCTGCAAATGCTTAGAT
TGAAATTTGATGAAGCTAAACCTAGTATCCAGAACCTGATCCAAGAGATGAAGGTGAGCCATCCACAACT
GAGAATCAGCAGTGACCAAGTGGATCTTAAGAATCCTCCTCTTCCTCATTTCATTTTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215150 representing NM_177690
Red=Cloning site Green=Tags(s)

MEHFDPLGFNLKDVLRKLDKTELNNFKRTLRSCSLPDTMKQINKLTMDLANGAQLAEILTDHCPSAWIKR
VTVQILEEINRVDLAELVVKQIEEAVLKVPEEKVSSKPREPSGTLTFPWNFVQGAKRPEDKQKEEWKTRY
TAKWKQNFWPKCNKEIYVVTESYKTLLALCNPKIETPFAHAIVLHGPPGSGKTTMAKQLMLEWSESKQAQ
IFSCAFYISCREVNNTKPCTFAHLLSMDNPSWRDCVIRDLILGKEFLFVVDGFDELTFPAGALIRDLCGD
WNTVKPVEVLLGSLLKRKMAPHATLLVTTRTQSLHQIFVMMDQPLLVETLGFLEQEKQEYFQKYFEDEEG
EEEDKGEGKALRALKEVRCNADLYQMASLPTACGIFCLCLELRMKKGEDLSLTCQTYTSMFLNFLCEVFS
SETCEDHLNEEFQILFKKICILAANSLLEQVPILCEEDFLTLKLNLNNLHPMVCRHILFKDSSSTHCLSF
ICLGIQQLLAAIIFVQELGQESKGVSKYSIQNMLSREARLKNPDLSGLLPFVFGLLNETRIQELKTTFGC
QISTEVKRKFLECESGENKPLLLLMNMQEILSCLYESQEEGFVKEAMVLFEDISLHLKTSTDLIHASFCL
KNSQNLQTMSLKVEKAVFPENVAALESTAKHQRSPDEQRMLTFWTDFCDTFNSNKKLVFLDIHESFLNSS
ALEILCEKLPSASCCLQKVVLKNISPDDAYEKLCLIFNGYKTISHLILQGGNLDSMHHSLCEVLKNPACN
LKFLSLGSCSTAAQKWDDFFPVLKVNQSLIFLDLTDNSLLDKSAKLLCNIWKEPKCILQRVSLENCQLTE
ACCKDLSSVLMVSRTLTHLSLANNKLGDNGVKNLCESISCTECNLQTLVLWSCNITNAGCHYLSKMLKQT
LSLKHLDLGLNRIGTKGAKFLCEALKNPKSKLKSLWLCGCSITPLNCQDFSETLRSNKSLNTLDLSQNVL
GTDAIKTFCEALKLQICPLQMLRLKFDEAKPSIQNLIQEMKVSHPQLRISSDQVDLKNPPLPHFIF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177690
ORF Size 3138 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177690.3, NP_808358.2
RefSeq Size 3404 bp
RefSeq ORF 3141 bp
Locus ID 232827
Cytogenetics 7 A1
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Documents
Resources
"NM_177690" in other vectors (4)
SKU Description Size Price
MC223378 Nlrp2 (untagged) - Mouse NLR family, pyrin domain containing 2 (Nlrp2), (10ug) 10 ug
$1,002.00
MR215150 Nlrp2 (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 2 (Nlrp2) 10 ug
$589.00 $911.00
MR215150L3 Lenti ORF clone of Nlrp2 (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 2 (Nlrp2) 10 ug
$1,211.00
MR215150L4 Lenti ORF clone of Nlrp2 (mGFP-tagged) - Mouse NLR family, pyrin domain containing 2 (Nlrp2) 10 ug
$1,211.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.