Slc35d1 (NM_177732) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG215146
Slc35d1 (tGFP-tagged) - Mouse solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter) member D1 (Slc35d1), (10ug)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc35d1
Synonyms AI834976; C330011J09; mKIAA0260; UGTREL7
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215146 representing NM_177732
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAAGTCCACAGACGTCAGCATGCTCCGGTTAAAGGAGAAGCCCCCGCGAAATCCTCCACACACA
GAGATGAGGAGGAGCTGGGGATGGCGCCGGCCGAAACGCTAACGGTGTTCCTGAAGCTGCTGGCCGCCGG
TTTCTACGGCGTGAGCTCCTTCCTCATCGTGGTGGTCAACAAGAGCGTGCTCACCAACTACAGATTTCCA
TCCTCGCTATGTGTTGGACTTGGCCAGATGGTGGCCACGGTAGCGGTTCTCTGGGTGGGAAAGACACTCA
GAGTAGTCAAGTTTCCTGACTTCGACAGAAATGTACCTCGAAAGACGTTTCCACTACCTCTACTATATTT
TGGGAACCAAATCACGGGACTGTTCAGCACAAAGAAACTGAACCTGCCCATGTTTACAGTTCTGAGAAGG
TTCTCCATTCTGTTTACAATGTTTGCTGAAGGCGCTCTACTCAAGAAGACTTTCTCCTGGGGTATTAAGA
TGACTGTATTTGCAATGATCATCGGAGCCTTCGTAGCTGCCAGCTCTGACTTGGCCTTTGATCTGGAAGG
ATATGTTTTTATTTTGATCAATGATGTCCTGACAGCAGCAAATGGTGCATACGTGAAACAGAAGTTAGAT
TCAAAAGAGCTGGGAAAGTATGGCCTGCTCTATTACAATGCACTGTTCATGATTCTGCCCACCCTGGCCA
TTGCGTATTTCACAGGAGATGCACAAAAGAATATATTAATAACTTATATTGGAATGGTCTTTGGTGGAGA
TTATATTTTCACATGGACAAATTTTATCGGCCTAAATATCAGTATTGCTGGAAGCCTGGTGTACTCTTAC
ATCACTTTCACGGAAGAGCAGCTGAGCAAGCAGTCAGAGGCCAGTAACAAGCTGGACACGAAGGGAAAAG
GAGCAGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215146 representing NM_177732
Red=Cloning site Green=Tags(s)

MAEVHRRQHAPVKGEAPAKSSTHRDEEELGMAPAETLTVFLKLLAAGFYGVSSFLIVVVNKSVLTNYRFP
SSLCVGLGQMVATVAVLWVGKTLRVVKFPDFDRNVPRKTFPLPLLYFGNQITGLFSTKKLNLPMFTVLRR
FSILFTMFAEGALLKKTFSWGIKMTVFAMIIGAFVAASSDLAFDLEGYVFILINDVLTAANGAYVKQKLD
SKELGKYGLLYYNALFMILPTLAIAYFTGDAQKNILITYIGMVFGGDYIFTWTNFIGLNISIAGSLVYSY
ITFTEEQLSKQSEASNKLDTKGKGAV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177732
ORF Size 918 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177732.5
RefSeq Size 2893 bp
RefSeq ORF 921 bp
Locus ID 242585
UniProt ID A2AKQ0
Cytogenetics 4 C6
Summary Transports both UDP-glucuronic acid (UDP-GlcA) and UDP-N-acetylgalactosamine (UDP-GalNAc) from the cytoplasm into the endoplasmic reticulum lumen. Plays a role in chondroitin sulfate biosynthesis, which is important for formation of cartilage extracellular matrix and normal skeletal development.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_177732" in other vectors (4)
SKU Description Size Price
MC213148 Slc35d1 (untagged) - Mouse solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1 (Slc35d1), (10ug) 10 ug
$330.00
MR215146 Slc35d1 (Myc-DDK-tagged) - Mouse solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1 (Slc35d1) 10 ug
$289.00 $300.00
MR215146L3 Lenti ORF clone of Slc35d1 (Myc-DDK-tagged) - Mouse solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1 (Slc35d1) 10 ug
$600.00
MR215146L4 Lenti ORF clone of Slc35d1 (mGFP-tagged) - Mouse solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1 (Slc35d1) 10 ug
$600.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.