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Spg20 (NM_001144988) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215145
Spg20 (tGFP-tagged) - Mouse spastic paraplegia 20 spartin (Troyer syndrome) homolog (human) (Spg20) transcript variant 3, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $772.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Spg20
Synonyms AI840044; C79168; mKIAA0610; SPARTIN; TAHCCP1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215145 representing NM_001144988
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGAGAACCAGAAAATGGAGAACCTGCTGAAATTAAGATCATCAAGGAAGCATACGAGAAGGCCT
TTATGTTTGTTAATAAAGGACTGAACACGGACGAGTTAGGGCAGAAGGAAGAAGCAAAGAACTATTACAA
GCAAGGAATAGGACACCTGCTTAGGGGCATCAGCATCGCGGCTGCAGAGCCGGGGCACACAGGCCCTGCG
TGGGAGGCTGCCAGGCAGATGCAACAGAAAATGAAGGAAACGCTTCAGAACGTCCGCACCAGGCTAGAGA
TCCTGGAGAAGGGCCTTGCCACTTCTCTACGGAATGACCTTCAGGATGTGCCCAAGCTATACCCAGAGTT
CCCGCCTAAAGACGCGTGCAAGAAGTCCCCAGAGCAGGAGTCCGTTAGTACAGCGCCCCAGCGTGCTGAA
GTCGATGGGAGCGCCTCAGCCGCGTGTGCCGGCCCAAGTGGTGCGCCTTCTGCTCTGCCCGTACCATCGC
CCAGCTGCCCTGCCGAAGCGCCACCAGCCTACAGCCCACAGGCTGCTGAGGGCCACTACACTGTTTCCTA
TGGAACAGATTCCGGGGAGTTTTCATCCGTGGGAGAAGACTTCTACAGGAACCGTTCCCAGCCGCCCCCT
CTTGAGACCTTGGGGCTCGATGCAGATGAGTTGATCTTGATACCAAACGGAGTGCAGATCTTTTTTGTAA
ACCCTGCGGGAGAGGTCAGTGCTCCCTCGTACCCTGGGTATCTGCGGATCGTGCGGTTCTTGGATAATTC
TCTGGATACAGTTCTGAACCGCCCTCCTGGGTTTCTCCAGGTGTGTGACTGGTTATACCCTCTAGTTCCT
GATAGGTCACCAGTTCTGAAGTGCACTGTGGGAGCCTACATGTTTCCAGATACCATGTTACAAGCTGCTG
GGTGCTTCGTAGGGGTTGTTCTTTCATCTGAGCTCCCAGAAGATGACAGAGAGCTGTTTGAGGATCTGTT
GAGACAGATGTCTGACCTTCGGCTCCAGACCAAAGATTCAAGTGAAGAAGTTAATCTGAGCCAGATTGTG
CCCTGTGAGCCGAGTTCAGAAGAAAAATCGAAAGAATTGCCTGAATGGAGCGAGAAAGTGGCCCACAACA
TCCTGTCAGGTGCTTCCTGGGTGAGCTGGGGTTTAGTCAAAGGTGCTGAATTTACTGGCAAAGCAATTCA
GAAAGGTGCTTCTAAACTCAGAGAGCGCATTCAGCCGGAGGAAAAGCCAGTGGAGGTGAGCCCAGCTGTC
ACCAGGGGTCTCTACATAGCGAAGCAGGCAACCGGAGGAGCAGCCAAAGTCAGCCAGCTCCTGGTTGACG
GCGTCTGCACAGTAGCGAATTGTGTTGGTAAAGAACTTGCTCCACACGTCAAGAAGCACGGAAGCAAACT
GGTTCCGGAATCCCTGAAGAGGGACAAAGACGGGAAATCTGCCCTGGATGGCGCCATGGTCGTGGCAGCG
AGTAGTGTTCAAGGATTCTCAACTGTCTGGCAAGGGTTGGAATGTGCAGCTAAATGCATCGTTAACAACG
TGTCTGCAGAAACTGTACAGACTGTGAGATACAAGTATGGGCACAACGCAGGAGAAGCTACACATAACGC
AGTGGATTCAGCTATCAATGTTGGGCTCACTGCCTACAATATTGACAACATCGGCATCAAAGCCATGGTG
AAGAAAACTGCCAAGCAGACGGGACACACTCTCCTCGAGGACTACCAGATCGTTGAGCGCCCTCAGAGGG
AGAGTCAGGGAGGAGCCACAAGCACAGAAGGGAGAAGAGACATAGGAAAGCAGGTGGAGGAAGAGAAGCC
AGGGGCAGGAAAGAAGGACAAA

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215145 representing NM_001144988
Red=Cloning site Green=Tags(s)

MEREPENGEPAEIKIIKEAYEKAFMFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISIAAAEPGHTGPA
WEAARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQDVPKLYPEFPPKDACKKSPEQESVSTAPQRAE
VDGSASAACAGPSGAPSALPVPSPSCPAEAPPAYSPQAAEGHYTVSYGTDSGEFSSVGEDFYRNRSQPPP
LETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVP
DRSPVLKCTVGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQTKDSSEEVNLSQIV
PCEPSSEEKSKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAV
TRGLYIAKQATGGAAKVSQLLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKRDKDGKSALDGAMVVAA
SSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHNAGEATHNAVDSAINVGLTAYNIDNIGIKAMV
KKTAKQTGHTLLEDYQIVERPQRESQGGATSTEGRRDIGKQVEEEKPGAGKKDK

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001144988
ORF Size 1842 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001144988.1, NP_001138460.1
RefSeq Size 3626 bp
RefSeq ORF 1845 bp
Locus ID 229285
UniProt ID Q8R1X6
Cytogenetics 3 C
Summary May be implicated in endosomal trafficking, or microtubule dynamics, or both. Participates in cytokinesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001144988" in other vectors (4)
SKU Description Size Price
MC219762 Spg20 (untagged) - Mouse spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 3, (10ug) 10 ug
$630.00
MR215145 Spg20 (Myc-DDK-tagged) - Mouse spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 3 10 ug
$289.00 $572.00
MR215145L3 Lenti ORF clone of Spg20 (Myc-DDK-tagged) - Mouse spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 3 10 ug
$872.00
MR215145L4 Lenti ORF clone of Spg20 (mGFP-tagged) - Mouse spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 3 10 ug
$872.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.