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Parp10 (NM_001163575) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215096
Parp10 (tGFP-tagged) - Mouse poly (ADP-ribose) polymerase family member 10 (Parp10) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,079.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Parp10 or NM_001163575"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Parp10
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215096 representing NM_001163575
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGGTGGAGGCAGGGGCAGCCTTGGAGCTCCGGGGACTGCCCCCAGAAATACCAGATGAGCTCA
TTACTCTGTACTTTGAAAACCACCGTCGCTCGGGTGGAGGACTTCTATTGAGCTGGCAGAGACTTGGCTG
TGGGGGTGTCCTCATCTTTCAAGATCCTGCAGATGCAAAGAGGGTCTTAGCCCAGGCAGAGCACCGACTA
CATGGTGTCCGACTGAGCCTGAGGCCAGCCCCACCGCGTGCCCCAGAACGTGTGCTTCTCCAACACCTGC
CACCTGGCACTTCACCTCTGAGTCTGGAGCAACATGTTCAGGCCTTGCTGGGTGCTGCAGGACACCCCGT
GCAGACTTGCCATGCCCTGGCCAGTCCTCGGCAGGACTGTGCCCTGGTCCAGTTATCCACGCCCCTTTCT
GAGGCAGAGGTCAGTGCCCTGGCAGAACAGGCCCGGAATCTGCCCCTGAATGGAGCCACTGTGTCCTTGG
CCTGGGTGCCCCAGACCCGAGCAGTACGTGTGGTGGACAGTGCTTCTCCTGTGGACCTGTTATTGTTGGA
GCTCTACCTGGAAAATGAGCGTCGCAGTGGTGGCGGACCCCTGGAGGGTCTGCGCAGCCTGCCTGGGCAG
CTAGGCACTGTTATTTCCTTTCAGCAATGGCAGGTGGCTGAACGGGTGCTGAAGCAGAAACACTGGCTAC
AGGGCATTGAGTTGAGCCTTGTCCCTCACTATGATGTCTTAGAGCCAGAAGCGCTCGCTGAGGGCGTCAG
TGGCAGGGACCATTCAGCTACTCAAGAATCTGGAGTCATTGGTCATGCGCCTACAGGAACTGGAGGACTG
GCCGGGGCTCTGACCATGGCTGTGGGCTCTGGGGAGGCACCACAACAATTAGGAACCCTTCTAAGGGCAG
GTCCTGTAGGAGCTCCAGGACAAGCCCTGCCAGTTGACTCGGGATCAATTAGGATCCAGGGGAGTATGGG
GTCTACAAGCCCAGTCGATCCAGTAGAGAGCTCTACAGAGCTCCCAGAGCAAGTAGGACCCATGGCCTCA
GACTCAGTGGGTGTTCAGGAGCAGGAGGGCCTGGGGGAAGTTGCCACAGGGCAAGAGGGGCTAATGGGTC
TAGTGGGGACAGCCATGGAGTCTGTAGAGACTGGGCTGGAGAGCCCTGGGTATGGGGAGATGCAGAAGCA
AGAGGGCCTGGTGGAAATGGTGATGTCAGTGGAACCAGGAGCTGTGCGCTACCTGCAGCTTTATTATGAG
GACCTCCTCGCCAGCCTAGAAGATGTTGCCCTTTTCCCACTTGAAGGAACGGATGTGACTGGCTTTCGGC
TGTGTGGAGCCAGGGCCCCCTGCCAGGCAGCCCAAGAGTTGCTTCAGAGTCTGCTGGGCAGCATTAGCTG
TCACACGCTGAACATGAAGCACCCGGGCAGTGCCAGGTTCCTGCTGGGTGTGGAGGGGCAGCACCTTCTT
CACAGACTGGAGGCTCAGTTCCAGTGTGTCTTCGGGACAGAACATCTGGCCAGCGCCACCCTGGACATAG
ACCCTGAAAGGACAGATCCCACTGAGGCCCTCCAAGTCCTCCATGGCCACATTACAGGCATCGATCAGGA
GAGCTTGAGACTGGAGGACGTCCGAGAGCTACTGGCTACCCTGGAGAGCCCACATGGAGGGGAGGACAGG
GTGCCTCTGGAGATGGAGAAGGAGAAACCTGGAGGACCAGGAGAGACTGTTGTAGAGCAGCAGGAGGAAA
TCCCCACACTTGAGGCTGAGGAGGAGCCTGTGGCCCTTAGCACCGGGGCACGTGGGCAGTTAGAAGAAGA
AGCCACACTTCAACTGGCTATCCACCGGTCCTTGGAGTCTCAAAGCCAGGTGGCAGATCAGCAGGAGGCT
AATGCACTAAGGCGAGCCATGGCCCTTTCCCTGTTAGAAGCAGAAGAGGCCCTGGATGAGGACACTGGGG
GCGAGGCCCAGCTGGTGGTGCACACATCCTTTGAGCAGGATGTGGATGAATTGAACCAGGCCTTATCCAA
TGCCTTGGAGGCACACCTTCGGGAGGAGACAGTGAGTCTCCAAGGGCGCATGCTACCCCCGGAGCTGGGG
GCTCGTCTGGAAAGGTGTCATGATGTGAGTGCTACCCTCCGTGGCGACCGAGTTGTCCTCCGTGGCTTTG
GGGTCCAGCCTGCCCGCGCGGCTCGCCACTTAGCTGCTCTGCTTGTTGACCCCTGGGACCAGAATTTGAC
CTTTCCTTTGGAGGCTTCAAAACCCAACTTGTCTGAGCAGGGGCTGAAGGAACCCTTGGGCAGACTGGAG
GCCCTGGAAGAGAATTCCCAGGAGTTCCAAGATGTGGTGCGGGCCTTTTATAGCACCCTGGATGCTGTCC
ACGGCAGGATCCGCATTGTTCGAGTAGAACGCGTGTCACACCCATTGCTGCAGCAACAGTACCAGCTGCA
CCGGGAGCGTTTAATGCAGAGCTGCCAGCAACGCCCAGTGGAGCAGGTCCTCTACCATGGCACTTCAGAG
TCTGCAGTGCTCGACATCTGTGCGCACGGCTTCAATCGCAGTTTCTGTGGCCGAAACGGCACACTCTACG
GACAGGGCGTGTACTTCGCCAAGCGTGCCTCTCTGTCGGTACTGGATCGCTACTCGCCTCCCAACGCAGA
AGGCTACAAGGCAGTGTTTGTGGCACAGGTGCTGACGGGTGACTATGGACAGGGTTCCCGCGGTCTGAAG
GCACCTCCTTTGAGGGTCTCCGGTCAGGTGCTTCGCTACGACAGCGCTGTGGACTGCCTTCAGCAGCCCA
GAATCTTTGTCATCTTTCACGATACCCAAGCTTTACCGACTCATCTAATCACCTGCAAGAACATACTCCC
GGGTACCCCC

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215096 representing NM_001163575
Red=Cloning site Green=Tags(s)

MAEVEAGAALELRGLPPEIPDELITLYFENHRRSGGGLLLSWQRLGCGGVLIFQDPADAKRVLAQAEHRL
HGVRLSLRPAPPRAPERVLLQHLPPGTSPLSLEQHVQALLGAAGHPVQTCHALASPRQDCALVQLSTPLS
EAEVSALAEQARNLPLNGATVSLAWVPQTRAVRVVDSASPVDLLLLELYLENERRSGGGPLEGLRSLPGQ
LGTVISFQQWQVAERVLKQKHWLQGIELSLVPHYDVLEPEALAEGVSGRDHSATQESGVIGHAPTGTGGL
AGALTMAVGSGEAPQQLGTLLRAGPVGAPGQALPVDSGSIRIQGSMGSTSPVDPVESSTELPEQVGPMAS
DSVGVQEQEGLGEVATGQEGLMGLVGTAMESVETGLESPGYGEMQKQEGLVEMVMSVEPGAVRYLQLYYE
DLLASLEDVALFPLEGTDVTGFRLCGARAPCQAAQELLQSLLGSISCHTLNMKHPGSARFLLGVEGQHLL
HRLEAQFQCVFGTEHLASATLDIDPERTDPTEALQVLHGHITGIDQESLRLEDVRELLATLESPHGGEDR
VPLEMEKEKPGGPGETVVEQQEEIPTLEAEEEPVALSTGARGQLEEEATLQLAIHRSLESQSQVADQQEA
NALRRAMALSLLEAEEALDEDTGGEAQLVVHTSFEQDVDELNQALSNALEAHLREETVSLQGRMLPPELG
ARLERCHDVSATLRGDRVVLRGFGVQPARAARHLAALLVDPWDQNLTFPLEASKPNLSEQGLKEPLGRLE
ALEENSQEFQDVVRAFYSTLDAVHGRIRIVRVERVSHPLLQQQYQLHRERLMQSCQQRPVEQVLYHGTSE
SAVLDICAHGFNRSFCGRNGTLYGQGVYFAKRASLSVLDRYSPPNAEGYKAVFVAQVLTGDYGQGSRGLK
APPLRVSGQVLRYDSAVDCLQQPRIFVIFHDTQALPTHLITCKNILPGTP

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001163575
ORF Size 2880 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163575.1, NP_001157047.1
RefSeq Size 3307 bp
RefSeq ORF 2883 bp
Locus ID 671535
UniProt ID Q8CIE4
Cytogenetics 15 D3
Summary ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate and aspartate residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Catalyzes mono-ADP-ribosylation of GSK3B, leading to negatively regulate GSK3B kinase activity. Involved in translesion DNA synthesis in response to DNA damage via its interaction with PCNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001163575" in other vectors (4)
SKU Description Size Price
MC222860 Parp10 (untagged) - Mouse poly (ADP-ribose) polymerase family, member 10 (Parp10), transcript variant 1, (10ug) 10 ug
$968.00
MR215096 Parp10 (Myc-DDK-tagged) - Mouse poly (ADP-ribose) polymerase family, member 10 (Parp10), transcript variant 1 10 ug
$589.00 $879.00
MR215096L3 Lenti ORF clone of Parp10 (Myc-DDK-tagged) - Mouse poly (ADP-ribose) polymerase family, member 10 (Parp10), transcript variant 1 10 ug
$1,179.00
MR215096L4 Lenti ORF clone of Parp10 (mGFP-tagged) - Mouse poly (ADP-ribose) polymerase family, member 10 (Parp10), transcript variant 1 10 ug
$1,179.00

file-alt Citations

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