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Olfr1509 (NM_020514) Mouse Tagged ORF Clone

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SKU
MG215055
Olfr1509 (tGFP-tagged) - Mouse olfactory receptor 1509 (Olfr1509), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfr1509
Synonyms Mor83; MOR244-3; Or83
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215055 representing NM_020514
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGCTCTAAATCAAACAAGAGTGACTGAATTTATCTTCCTGGGCCTCACTGACAACTGGGTGTTGG
AGATTCTGTTTTTCGTACCATTTACAGTCACTTATATGTTAACACTTTTGGGGAACTTCCTCATTGTTGT
TACCATAGTCTTCACGCCACGTCTCCACAATCCCATGTACTTCTTTCTGAGCAATCTGTCCTTCATTGAC
ATCTGCCACTCATCTGTCACCGTGCCCAAGATGCTTGAGGGTTTGCTTTTAGAGAGGAAGACCATTTCCT
TTGACAATTGCATTGCACAGCTCTTCTTCCTACATCTCTTTGCTTGTTCTGAGATCTTTCTGCTGACGAT
TATGGCGTATGATCGTTATGTGGCTATCTGCATCCCATTGCATTACTCCAATGTGATGAACATGAAGGTC
TGTGTACAGCTTGTCTTTGCACTCTGGCTGGGGGGCACTATTCATTCACTTGTGCAGACCTTCTTGACTA
TTCGTCTACCCTACTGTGGCCCAAACATTATCGATAGCTACTTCTGTGACGTACCTCCTGTCATCAAGCT
GGCCTGCACAGATACATACCTTACAGGGATTCTGATCGTGTCCAATAGTGGAACCATCTCCCTCGTATGT
TTTCTAGCCTTGGTCACTTCTTACACAGTCATCCTGTTTTCTCTTCGAAAACAGTCTGCAGAAGGTCGTC
GGAAAGCCCTGTCCACCTGCTCAGCCCACTTCATGGTGGTCGCCCTGTTCTTTGGGCCATGTATCTTCCT
CTACACTCGGCCAGATAGCAGCTTCTCCATCGACAAGGTGGTATCGGTTTTCTACACAGTGGTCACCCCT
TTGTTGAATCCTCTCATTTACACTTTGAGGAATGAGGAAGTGAAAACTGCCATGAAGCATCTCAGGCAGA
GACGAATTTGCTCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215055 representing NM_020514
Red=Cloning site Green=Tags(s)

MGALNQTRVTEFIFLGLTDNWVLEILFFVPFTVTYMLTLLGNFLIVVTIVFTPRLHNPMYFFLSNLSFID
ICHSSVTVPKMLEGLLLERKTISFDNCIAQLFFLHLFACSEIFLLTIMAYDRYVAICIPLHYSNVMNMKV
CVQLVFALWLGGTIHSLVQTFLTIRLPYCGPNIIDSYFCDVPPVIKLACTDTYLTGILIVSNSGTISLVC
FLALVTSYTVILFSLRKQSAEGRRKALSTCSAHFMVVALFFGPCIFLYTRPDSSFSIDKVVSVFYTVVTP
LLNPLIYTLRNEEVKTAMKHLRQRRICS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020514
ORF Size 924 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020514.2, NP_065260.2
RefSeq Size 1010 bp
RefSeq ORF 927 bp
Locus ID 57271
UniProt ID Q7TQQ0
Cytogenetics 14 C2
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_020514" in other vectors (4)
SKU Description Size Price
MC210132 Olfr1509 (untagged) - Mouse olfactory receptor 1509 (Olfr1509), (10ug) 10 ug
$330.00
MR215055 Olfr1509 (Myc-DDK-tagged) - Mouse olfactory receptor 1509 (Olfr1509) 10 ug
$289.00 $300.00
MR215055L3 Lenti ORF clone of Olfr1509 (Myc-DDK-tagged) - Mouse olfactory receptor 1509 (Olfr1509) 10 ug
$600.00
MR215055L4 Lenti ORF clone of Olfr1509 (mGFP-tagged) - Mouse olfactory receptor 1509 (Olfr1509) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.