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Tmem104 (NM_001033393) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215039
Tmem104 (tGFP-tagged) - Mouse transmembrane protein 104 (Tmem104), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem104
Synonyms C630005D06Rik; mFLJ00021
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215039 representing NM_001033393
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGCGAGATCACAGAGACCGGAGAGCTCTATTCTCCTTATGTCGGGCTGGTGTACATGTTTAACC
TCATCGTGGGCACAGGTGCGCTCACCATGCCCAAGGCCTTTGCCACCGCCGGGTGGCTCGTCAGCCTCGT
TCTGCTGGTATTCGTAGGCTTCATGAGCTTTGTGACAACCACCTTTGCGATGGAAGCCATGGCTGCAGCC
AATGCCCAGCTCCGCTGGAAGAGGATGGAGACCCACAAGGAAGAGGATGACGAAGACTCGTCCACAGCCT
CAGACAGTGACCTCCTCAGCCAAGACAACTACGAGAGGGCAGAGAAACGCCCCATCCTGTCTGTGCAGAG
ACGCTCATCTGCCAACCTTTTTGAAATCACAGACCGGGTGGAAATGGGACAGATGGCTTCCATGTTCTTC
AATAAAGTGGGGGTCAACTTGTTCTATTTCTGCATCATTACTTACCTGTATGGAGACCTCGCCATCTACG
CGGCCGCGGTGCCCGTGTCCCTCATGCAGGTGACCTGCAGCGTCTCTGGAAATGACTCTTGCAGCGTGGA
TACAGACGCCAGATACAACGACACTGATCTGTGCTGGGGCCCCCTGCGCCGGGTGGATGTCTACCGAATC
TATCTGGCCATCTTCACCGTCCTTCTTGGCCCGTTCACCTTCTTTGATGTCCAGAAGACTAAGTACCTGC
AGATCTTGACCTCTATGATGAGATGGATCGCCTTTGCCATCATGATTGTGCTGGCCCTGGTGCGCATCGG
GAAAGGACAAGGAGAGGGGCACCCGCCCCTAGCCAACTTCTTGGGGGTCCAGAACCTGTTTGGGGTGTGT
GTCTACTCGTTCATGTGCCAACACTCCCTGCCGTCCCTCATCACTCCCATCTCCTCCAAGCGCCACATCA
CGCGGCTGCTGTTCCTGGACTACGCACTGATCCTGGCCTTCTACGGCCTCCTCTCCTTCACGGCTATCTT
CTGCTTCCGTGGGGACAGCCTCATGGACATGTACACCCTCAACTTCGCGAGGTGTGACGTCGTGGGCCTT
GCTGCAGTCCGGTTCTTTCTGGGCCTCTTCCCTGTGTTCACCATCAGCACCAACTTCCCCATCATCGCGG
TGACCCTGCGCAACAACTGGAAGACGCTCTTCCACCGTGAAGGTGGCACCTACCCATGGGTGGTAGACCG
AGTGGTGTTCCCCACGATCACCCTGGTGCCTCCCATTCTGGTGGCCTTCTGCACCCATGACCTGGAGTCC
CTGGTGGCCATTACAGGAGCCTATGCGGGCACCGGCATCCAGTACGTCATCCCTGCCTTCCTGGTGTACC
TCTGCCGCAAGGACACCCAGCTGACCTTTGGCTATGGAACTGTCAACAAGCATAGGTCCCCGTTCCGCCA
CACCTTCTGGGTGGCCTTTGTGCTGCTGTGGGCTTTCTCCTGCTTCTTCTTTGTCACGGCCTACATCGTC
CTCAAGGAGACCCAGCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215039 representing NM_001033393
Red=Cloning site Green=Tags(s)

MAGEITETGELYSPYVGLVYMFNLIVGTGALTMPKAFATAGWLVSLVLLVFVGFMSFVTTTFAMEAMAAA
NAQLRWKRMETHKEEDDEDSSTASDSDLLSQDNYERAEKRPILSVQRRSSANLFEITDRVEMGQMASMFF
NKVGVNLFYFCIITYLYGDLAIYAAAVPVSLMQVTCSVSGNDSCSVDTDARYNDTDLCWGPLRRVDVYRI
YLAIFTVLLGPFTFFDVQKTKYLQILTSMMRWIAFAIMIVLALVRIGKGQGEGHPPLANFLGVQNLFGVC
VYSFMCQHSLPSLITPISSKRHITRLLFLDYALILAFYGLLSFTAIFCFRGDSLMDMYTLNFARCDVVGL
AAVRFFLGLFPVFTISTNFPIIAVTLRNNWKTLFHREGGTYPWVVDRVVFPTITLVPPILVAFCTHDLES
LVAITGAYAGTGIQYVIPAFLVYLCRKDTQLTFGYGTVNKHRSPFRHTFWVAFVLLWAFSCFFFVTAYIV
LKETQL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033393
ORF Size 1488 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033393.2, NP_001028565.1
RefSeq Size 4583 bp
RefSeq ORF 1491 bp
Locus ID 320534
UniProt ID Q3TB48
Cytogenetics 11 E2
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Documents
Resources
"NM_001033393" in other vectors (4)
SKU Description Size Price
MC217080 Tmem104 (untagged) - Mouse transmembrane protein 104 (Tmem104), (10ug) 10 ug
$503.00
MR215039 Tmem104 (Myc-DDK-tagged) - Mouse transmembrane protein 104 (Tmem104) 10 ug
$289.00 $457.00
MR215039L3 Lenti ORF clone of Tmem104 (Myc-DDK-tagged) - Mouse transmembrane protein 104 (Tmem104) 10 ug
$757.00
MR215039L4 Lenti ORF clone of Tmem104 (mGFP-tagged) - Mouse transmembrane protein 104 (Tmem104) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.