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Vrtn (NM_001033776) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215030
Vrtn (tGFP-tagged) - Mouse RIKEN cDNA 7420416P09 gene (7420416P09Rik) transcript variant 3, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $878.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vrtn
Synonyms 7420416P09Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215030 representing NM_001033776
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGTCTCGGGATCAGCTGGTGCAGCAGGTGCTGCGCGACCTGCAGGAGGCAGTGGAGTCCGAGGGCT
TAGAGGGTCTCATCGGTGCTGCCCTGGAGGCCAAGCAGGTCCTATCTTCCTTCACCCTTCCCATCTGCCA
GAAGGGCGGCCCTGGCGCTCAGGTGCTGGAGGTGGACTCAGTGGCCTTGAGTCTGTACCCGGAGGATGCT
CCTCGGAACATGCTGCCCTTGGTGTGCAAGGGTGAGGGGAGCCTGTTGTTTGAAGCGACCAGCCTGTTGT
TGTGGGGGCACACGGGTCTCAGCCTGGAGCTGCGAGCCCGCACTGTGGTGGAGATGCTGCTGCACAGGCA
CTATTATCTGCAGGGCATGATCGACTCCAAGGTGATGCTGCAGGCTGTGCGCTACTCCTTGTGCTCTGAG
GAGTCCCCTGAGATGACCAACTTATCTTTTGCCACACTGGAGGCCATCTTTGATGCTGATGTAAAGGCTA
CGTGCTTCCCAACGAGCTTCTCCAATGTGTGGCACTTGTATGCCCTCGCCTCCATCCTTGAATGCAACAT
CTACTCCATCTACCCCATGCGCAACATCAAGATCCGACCCTATTTTAACCGTGTTATCATGCCTCGCTGC
TCCACCCACGTGACCTCCATGCTGCACATCATGTGGGCTGGTCAGCCCCTCACTAGCCACCTCTTCCGTC
ACCAGTATTTTGCCCCTGTTGTTGGGCTGGAGGAGGTGGAGGCTGACTGTACTGCTAGCCTGAACCCCGT
TCCTCCAAACCTGGGTCCTCTGCTACCACCCGCTAAAACCCTGGAGCTGCTCAACCGTGAACCTGGCCTC
AGCTACTCCCACCTCTGTGACCGCATCAGCATCACCAAGAGCACCTTCTACCGCTGGCGGCGGCAGACCC
AAGAGCACCGTCAAAAGGTAGCCACCCGCTTCTCTGCCAAGCACTTCCTGCAGGACAGTTTCCACCGTGG
GGGCTTTGTGCCATTGCAGCAGTTCCTGCAGAGATTCCCTGAGATCTCCCGTTCTACCTACTATGCTTGG
AAGCATGAGTTGCTGGGCTCTGGTGCCAACTCGGCCCTGGGCCCGGCCACTCCATCCAGGGAGGCACTGG
CTGTGCCGGAGGTGGAGAGGCCACCGGGGAAAAAGGCTGCTGAGGAGGTGGGGTGCTCCTCACTGGCAGC
AGCGATGTTGAGCCCTCCTAGTATGATCTTAATGCAGCGTGCCAAGTCTTTCTTGGAGTATTGCATCTCT
CTGAACAAACTGGTACCATATCGCTGCTTCAAATGCAGGTTCCCTGGCATCTCGAGGTCTACCTACTACA
ACTGGAGGCGGAAAGCCCTCAGAAGGACCCCCAGTTTCAAGTTGTCCCAAGCAGCTTTTGAAACTGCTGA
GTCTCTCCAGCCAACAGATGTTGGGAAAGAGACCCCCTTCTCTTTGAAGAGAGAAGCGGGAGAGGAGGAG
ACAGGAAAAGCAGGAAGTGGGGCTCCTCTGACTTCCCGGGGGCTCATATCCCCAAAGATGCCCTTATCTC
GTTGGCAGAGACGACTACGCAGGGCTGCCCGCAAGCAGGTGCTAAATGGGCACCTACCCTTCTGTCGCTT
CCGCCTCCGCTATCCTAGTCTGTTACCTTCCACTTTTTGGGTATGGAAAAGTCTTTCCCGGAGATCGCCC
GGGATGCAGATTCCCTCTTTGAGCCAGAGGAGGCAGAAGCCTCAGGGACGGCAGAAGCCCGAGGGACGGC
AGAAGCCCGAGGAACAGCAGAAGCCCGAGGGACGGCAGAAGCCCGAGGGACGGCAGAAGCCAGTTGAACC
ACAGGCTATGGAAGCTGATCAGAATGTACCGGCTATGGTGGTTCCTCCAGCGGAGACACTGCCAGTGGCT
ACTTCTCCAGAAGATGTCCCAGGAGGGCCTTCCAGAGAGGGAAACATCATTCAAGAGGCAGCTATGACCC
AGAGCCAACCCCATAGTGGATCGTTGCCAAGCCAGACTCTGGCGGAGGCACCCGGGGGCAGTGATGGGCA
GGTGCTGGTGATGGACATGCTTACCACCACGAGGTTCAAGGCCCAGGCCAAGCTGTTCCTGCAGAAGCGG
TTCCAGTCTAAGACTTTCCCCTCCTACAAGGAGTTCCAGGCGCTCTTCCCCCTCACTGCCCGCTCCACCT
ACTACATGTGGAAGCGGGCGCTCTATGAAGGTCTCACACTGATTGATGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215030 representing NM_001033776
Red=Cloning site Green=Tags(s)

MTSRDQLVQQVLRDLQEAVESEGLEGLIGAALEAKQVLSSFTLPICQKGGPGAQVLEVDSVALSLYPEDA
PRNMLPLVCKGEGSLLFEATSLLLWGHTGLSLELRARTVVEMLLHRHYYLQGMIDSKVMLQAVRYSLCSE
ESPEMTNLSFATLEAIFDADVKATCFPTSFSNVWHLYALASILECNIYSIYPMRNIKIRPYFNRVIMPRC
STHVTSMLHIMWAGQPLTSHLFRHQYFAPVVGLEEVEADCTASLNPVPPNLGPLLPPAKTLELLNREPGL
SYSHLCDRISITKSTFYRWRRQTQEHRQKVATRFSAKHFLQDSFHRGGFVPLQQFLQRFPEISRSTYYAW
KHELLGSGANSALGPATPSREALAVPEVERPPGKKAAEEVGCSSLAAAMLSPPSMILMQRAKSFLEYCIS
LNKLVPYRCFKCRFPGISRSTYYNWRRKALRRTPSFKLSQAAFETAESLQPTDVGKETPFSLKREAGEEE
TGKAGSGAPLTSRGLISPKMPLSRWQRRLRRAARKQVLNGHLPFCRFRLRYPSLLPSTFWVWKSLSRRSP
GMQIPSLSQRRQKPQGRQKPEGRQKPEEQQKPEGRQKPEGRQKPVEPQAMEADQNVPAMVVPPAETLPVA
TSPEDVPGGPSREGNIIQEAAMTQSQPHSGSLPSQTLAEAPGGSDGQVLVMDMLTTTRFKAQAKLFLQKR
FQSKTFPSYKEFQALFPLTARSTYYMWKRALYEGLTLIDG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033776
ORF Size 2220 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033776.2, NP_001028948.1
RefSeq Size 3028 bp
RefSeq ORF 2223 bp
Locus ID 432677
UniProt ID Q3SYK4
Cytogenetics 12 D1
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Documents
Resources
"NM_001033776" in other vectors (4)
SKU Description Size Price
MC221267 Vrtn (untagged) - Mouse vertebrae development homolog (pig) (Vrtn), transcript variant 3, (10ug) 10 ug
$746.00
MR215030 Vrtn (Myc-DDK-tagged) - Mouse vertebrae development homolog (pig) (Vrtn), transcript variant 3 10 ug
$589.00 $678.00
MR215030L3 Lenti ORF clone of Vrtn (Myc-DDK-tagged) - Mouse vertebrae development homolog (pig) (Vrtn), transcript variant 3 10 ug
$978.00
MR215030L4 Lenti ORF clone of Vrtn (mGFP-tagged) - Mouse vertebrae development homolog (pig) (Vrtn), transcript variant 3 10 ug
$978.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.