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Tspan8 (NM_001168679) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215026
Tspan8 (tGFP-tagged) - Mouse tetraspanin 8 (Tspan8) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tspan8
Synonyms C76990; E330007O21Rik; Tm4sf3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215026 representing NM_001168679
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGTGTGAGCAGCTGTTTAAAGTATTCTATGTTTTTCTTCAACTTCTTGTTCTGGGTATGTGGTA
CACTGATCTTGGGACTGGCCATATGGGTGAGAGTAAGCAAAGATGGAAAAGAGATCATCACGTCAGGAGA
CTCTAGCACGAACCCCTTCATTGCTGTGAATATCTTGATTGCAGTTGGGTCTATCATCATGGTTCTAGGT
TTCCTGGGATGCTGTGGAGCTGTGAAAGAAAGCCGGTGCATGCTTCTCTTGTTTTTTATCGGCTTGCTGC
TGATTCTGATTCTGCAAGTGGCAGCAGGCATCCTAGGAGCCGCTTTCAAACCTGAGTATAACCGAATTCT
GAATGAAACGCTATATGAAAATGCTAAGCTTCTGTCGGACAACACTGATGAGGCAAAAGACTTCCAGAAG
GCCATGATTGTGTTTCAGTCGGAGTTCAAGTGCTGTGGCTTGGAGAATGGAGCTGCCGACTGGGGAAATA
ATTTTGTGGAGGCAAAAGAGTCATGTCAATGTACAGGAACTGACTGTGCAACTTATCAGGGGAGTTCCGT
TTACCCAAAGACCTGTCTTTCTTTGATCAAAGATCTGTTTGAAAAGAACATTATTATTGTCATTGGAATT
GCTTTTGGACTGGCCGTTATTGAGATTCTCGGTTTGGTGTTTTCTATGGTGCTATACTGCCAGATTGGAA
GCAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215026 representing NM_001168679
Red=Cloning site Green=Tags(s)

MAGVSSCLKYSMFFFNFLFWVCGTLILGLAIWVRVSKDGKEIITSGDSSTNPFIAVNILIAVGSIIMVLG
FLGCCGAVKESRCMLLLFFIGLLLILILQVAAGILGAAFKPEYNRILNETLYENAKLLSDNTDEAKDFQK
AMIVFQSEFKCCGLENGAADWGNNFVEAKESCQCTGTDCATYQGSSVYPKTCLSLIKDLFEKNIIIVIGI
AFGLAVIEILGLVFSMVLYCQIGSK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001168679
ORF Size 705 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001168679.1, NP_001162150.1
RefSeq Size 1380 bp
RefSeq ORF 708 bp
Locus ID 216350
UniProt ID Q8R3G9
Cytogenetics 10 D2
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Documents
Resources
"NM_001168679" in other vectors (3)
SKU Description Size Price
MR215026 Tspan8 (Myc-DDK-tagged) - Mouse tetraspanin 8 (Tspan8), transcript variant 2 10 ug
$289.00 $300.00
MR215026L3 Lenti ORF clone of Tspan8 (Myc-DDK-tagged) - Mouse tetraspanin 8 (Tspan8), transcript variant 2 10 ug
$600.00
MR215026L4 Lenti ORF clone of Tspan8 (mGFP-tagged) - Mouse tetraspanin 8 (Tspan8), transcript variant 2 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.