1700047I17Rik2 (NM_001100116) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG214994
1700047I17Rik2 (tGFP-tagged) - Mouse RIKEN cDNA 1700047I17 gene 2 (1700047I17Rik2), (10ug)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 1700047I17Rik2
Synonyms MGC61221
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214994 representing NM_001100116
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGTGAGTCGGGATGCGCGGCGGCGGGAGAGACCGAGGCAGCGGCCGCCACGGCGTTCCGAGACG
CGACACGGCAGATAAGTAATGAAAGAGGATTTGAGAATGTAGAACTGGGCGTCATGGGGAAGAAGAAGAA
AGTCCCGAGGAGGGTCATCCACTTTGTCAGTGGAGAGACAATGGAGGAGTACAGCACCGACGAGGACGAG
GTGGACGGCCTGGACAAGAAGGACGTGCTGCCCACTGTCGACCCGACAAAACTTACCTGGGGCCCCTACC
TATGGTTTTACATGCTGCGGGCCGCCACATCAACCCTCTCAGTGTGTGACTTTCTTGGAGAGAAGATTGC
GTCAGTTTTGGGCATCAGCACCCCAAAATACCAGTACGCCATTGATGAGTATTACCGGATGAAGAAGGAG
GAGGAGGAGGAGGAAGAAGAAAATAGGATGTCAGAAGAAGCAGAAAGACAATACCAACAAAACAAGCTGC
AGGCCGATTCCATTGTACAGACAGACCAACCAGAAACAGTGTCGTCCAGCTTTGTAAATATTAATTTTGA
AATGGAGGAAGACTGTGAAGCAATTAAGGAAAACAAACAGCGTCCAGTCTCTGTCCCACCA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214994 representing NM_001100116
Red=Cloning site Green=Tags(s)

MERESGCAAAGETEAAAATAFRDATRQISNERGFENVELGVMGKKKKVPRRVIHFVSGETMEEYSTDEDE
VDGLDKKDVLPTVDPTKLTWGPYLWFYMLRAATSTLSVCDFLGEKIASVLGISTPKYQYAIDEYYRMKKE
EEEEEEENRMSEEAERQYQQNKLQADSIVQTDQPETVSSSFVNINFEMEEDCEAIKENKQRPVSVPP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001100116
ORF Size 621 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001100116.1, NP_001093586.1
RefSeq Size 3863 bp
RefSeq ORF 624 bp
Locus ID 100101807
UniProt ID Q8BR63
Cytogenetics 12 C1
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_001100116" in other vectors (4)
SKU Description Size Price
MC215654 1700047I17Rik2 (untagged) - Mouse RIKEN cDNA 1700047I17 gene 2 (1700047I17Rik2), (10ug) 10 ug
$330.00
MR214994 1700047I17Rik2 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700047I17 gene 2 (1700047I17Rik2) 10 ug
$289.00 $300.00
MR214994L3 Lenti ORF clone of 1700047I17Rik2 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700047I17 gene 2 (1700047I17Rik2) 10 ug
$600.00
MR214994L4 Lenti ORF clone of 1700047I17Rik2 (mGFP-tagged) - Mouse RIKEN cDNA 1700047I17 gene 2 (1700047I17Rik2) 10 ug
$600.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.