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Trem3 (NM_021407) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214991
Trem3 (tGFP-tagged) - Mouse triggering receptor expressed on myeloid cells 3 (Trem3), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Trem3 or NM_021407"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trem3
Synonyms BB134760; TREM-3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214991 representing NM_021407
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCACCGCTGCTGCTATGGCTGGGGCTGATGCTCTGTGTCTCGGGACTCCAAGCTGGAGATGAGGAAG
AACACAAGTGTTTTCTGGAGGGCGAGAACCTGACCCTGACTTGTCCTTACAACATCATGCTATACTCACT
GAGCCTGAAGGCCTGGCAGCGGGTCAGAAGCCACGGTTCTCCAGAGACTCTGGTGCTCACAAACACCAGA
AAGGCAGACTTCAACGTGGCCAGGGCTGGGAAGTACTTGCTGGAGGATTATCCCACCGAATCTGTCGTCA
AGGTCACGGTGACTGGGCTGCAGAGGCAAGATGTGGGGCTGTACCAGTGTGTGGTCTACCTCTCTCCTGA
CAATGTTATCATTCTGCGTCAACGGATACGGCTGGCATGGTGTCAAGGGAAGCCAGTGATGGTGATCGTT
CTGACGTGTGGCTTCATACTAAACAAGGGCCTGGTCTTCTCAGTCCTGTTTGTCTTTCTCTGCAAAGCTG
GGCCTAAGGTGTTACAGCCTTCCAAGACATCCAAAGTACAGGGAGTCTCTGAGAAACAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214991 representing NM_021407
Red=Cloning site Green=Tags(s)

MSPLLLWLGLMLCVSGLQAGDEEEHKCFLEGENLTLTCPYNIMLYSLSLKAWQRVRSHGSPETLVLTNTR
KADFNVARAGKYLLEDYPTESVVKVTVTGLQRQDVGLYQCVVYLSPDNVIILRQRIRLAWCQGKPVMVIV
LTCGFILNKGLVFSVLFVFLCKAGPKVLQPSKTSKVQGVSEKQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021407
ORF Size 549 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021407.3, NP_067382.1
RefSeq Size 994 bp
RefSeq ORF 552 bp
Locus ID 58218
UniProt ID Q9JKE1
Cytogenetics 17 C
Summary The protein encoded by this gene is part of the immunoglobulin and lectin-like superfamily and is thought to function as part of the innate immune system. This gene forms part of a cluster of genes on mouse chromosome 17 thought to be involved in innate immunity. This protein has been reported to associate with the adaptor protein Dap-12 to form a receptor signaling complex that activates myeloid cells. provided by RefSeq, Jan 2013
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Documents
Resources
"NM_021407" in other vectors (4)
SKU Description Size Price
MC210168 Trem3 (untagged) - Mouse triggering receptor expressed on myeloid cells 3 (Trem3), (10ug) 10 ug
$330.00
MR214991 Trem3 (Myc-DDK-tagged) - Mouse triggering receptor expressed on myeloid cells 3 (Trem3) 10 ug
$289.00 $300.00
MR214991L3 Lenti ORF clone of Trem3 (Myc-DDK-tagged) - Mouse triggering receptor expressed on myeloid cells 3 (Trem3) 10 ug
$600.00
MR214991L4 Lenti ORF clone of Trem3 (mGFP-tagged) - Mouse triggering receptor expressed on myeloid cells 3 (Trem3) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.