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Tmem132c (NM_175432) Mouse Tagged ORF Clone

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SKU
MG214981
Tmem132c (tGFP-tagged) - Mouse transmembrane protein 132C (Tmem132c), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,164.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem132c
Synonyms 2810482M11Rik; 3230401P16; 4632425D07Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214981 representing NM_175432
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGGCGTGACAGCGGCCACGATGCGCTCCGAGGGCGCGGCCCCGAGGCGGGCGGCGCGGTACGGGG
CGCTGAGCCTGGTCCTAGCCACGCTACTGGGCCAAGTGACCGAAAGCCGAGGGGTCATGGATAATATACA
GAGATTCTCTTCATTGCCGCCGTACCTGCCCGTGAGCTTCCACGTCCTCAGAGCCGAGACTGCATTCTTC
CTAAAGGAGGCCAACCCCGACCCGCTGCGGAATGCCAGCCTGCAGTCCAGGGTGGAGTCTTTCTTCATCT
ACAAGGCCCAGCAGCCCCCGGTATTAAACGTCAGCTATGGGCCTTACTCTGCAGAAAAGGTCATCCCTCT
GGACTTGATGTTGAACCCCAACTTTTTAGGCCCAACCAGTAAGTTTCCCTTTGACTGGAGGCTGAAGGCC
TACATCCTTCAAGAGAAAGTCTACCTGAGCCATCCCAAAGTACAGGTGCTCTTCCACATCGTGGGCCGAG
ACTGGGATGACCACAGGGACGAGAAACTGCCCTGCCTGCGGGTCTTTGCGTTCAGAGACAGCCGGGAGGT
TCGAGGCAGCTGTCGTCTGGGTGGGCCCCTGGGACTGTGCGTGGCCCAGCTGGAGATGCTGCCTGGCTGG
TTCAGTCCCCCAGCGGTTGTATCTGGGCGCAGGAGGCCAGCAGAGCGGCCAGAGGGGAGTCCGGTGGAAC
TGTATTATGCTGTACAGCCAGGGGACGAGCGTGGTGACTGCACTGGAGGTGACACCAGGAAGGACAATGC
TATTCGTCCAGGAAAGGATGGACAGGAGGGCAGGACATCCCACCTACAGAAGATTGGCACCATTAGCCTT
TACCGCGCCCAGGACAGCACCCAGCTCAGCGAACTGCGCCTGGATGGCAATGTGGTCATCTGGCTGCCCT
CCCAGCCCGTCAAGCAGGGAGACATAGTCACCGCATCTGTCACCATCGCCAATAACTCTACTGTGGACCA
TTTCATCCTAAGAGCCAAGGTGAAGAAGGGGGTGAACATCCTGACCGTGCAGACCAGCGAGCCTCGGCAG
TGGGATGTGAGGCAAGAGGTGGGCAATGGAGGGAAGCACACCACCACCTCGGTGGCCTGCCAGCGCCTGG
GCCCTGGGGCACGAAATAGGAGCAGCAATTTATTCAGCGAGGTCATGCAGATGAATTTTGAAATCGCCAG
CTTCAGCAGCCTCTCGGGGACACAGCCTATCACATGGCAGGTGGAGTACCCGAGGAAGGGGGCCACGGAC
ATTGCTGTGTCGGAGATCTTCATCAGCCAGAAGGACCTAGTTGCCATCGTCCCCCTTGCTATGGACACTG
AACTCCTGAACACAGCCATCCTCACAGGGAAGACGGTGGCCATGCCTGTCAGGGTGGTGTCGGTGGAAGA
GAATAGCACCCTGAGGGACATCTCGGAGTTGGTGGAGTGCAAGGCCACAGACGAGAATGTCATCAAGGTC
TCGGACCACTGTGACTATGTCTTTGTCAATGGTAAAGAGATCAAGGGCAAGATGGACTCTGTGGTGAACT
TCACCTACCAGCACCTGAGCGCACCGCTGCATGTCACTGTGTGGGTGCCACGGCTTCCCCTGCAGATCGA
GGTCTCTGACACAGAACTCAGCCAGGTTAAGGGCTGGAGAGTCCCCATCGTGGCCAGCAAGAGGCCCACT
CGGGACAGTGAGGAGGAAGAAGAGGAAGAACAGAAAGGCCGGGGTTGTACCCTGCAGTTCCAGCATGCCA
CAGTGCGCGTCCTCACCCAATTTGTATCAGAGGGTGCTGGGCCCTGGGGCCAGCTGAGCCACCTTCTCAG
TCCAGACTGGCAGTTTGACATCACCCACCTGGTGGCTGACTTTATGAAGCTGGAGTCCCCACACATAGCC
ACCCTGCAGGACAGCAGGGTCTTGGTTGGGCGGGAAGTCGGAATGACCACCATCCAGGTGTTGTCTCCCC
TGTCCGACTCCATCTTGGCCGAGAAGACAGTAACTGTGCTGGATGACAAAGTATCTGTGACAGACTTAGC
TGTCCAGGTGGTGGCTGGGCTGTCTGTCACCCTACACCCCATCTCAGAGAACAACAAGGCCACCTCAGCT
GTGGCCATGGCAGAAGAGCTGCTACGTGCCCCAAAAAAGGAAGCTATAATCAGCACATGGCTCCAGTTCA
GTGATGGCTCAGTGACACCCCTGGATATCTACGACTCCAAGGACTTCTCCTTGACTGCCATCTCTTTGGA
CGAGGCTGTCGTGTCCATCCCCCAACCCCTCTCGCCTTGGTGGCCCACCGTGGTAGCTGAAGGAGAAGGC
CAGGGCCCACTGCTCCGGGTCGATATGTCCATTGCCGAAGCCTGTCAGAAATCCAAGCGCAAGAGTGTGC
TGGCTGTTGGCATTGGCCACGTGGGGGTCAAGTTTGGATGGGATGACGCTGACTCCAGCCAGACTGGAGA
AAAGGATGAGGAGGAGATCAAGAACCATGCCAGTGACCGTCGGCAGAAGATTCAGGACCTGGAACGCCCA
GGCCAGGATGAACTATACCATGGCAACTTTCCTGGGGATCGTGAAGAAGGAGCGCTGAGTGCTACCACCA
CTACCAAGTCCCTGCTGGATAACAACGTGGGGAAGAGTGGCAGGCGGGACGGGGCTAGGCTACACAGCAT
ACCCATTGACTTCACCAATTTCCCGGCCCATGTGGACCTCCCCAAGGCCAAGACCAGGGGCACACTGGAG
GAGAATGGTCTCATGCAGACAGCCCATGGCCTGAGTGACCTAGAGATTGGGATGTATGCCCTCCTAGGTG
TCTTCTGCCTGGCCATCCTTGTCTTTCTCATTAACTGCGCCACCTTTGCCTTCAAGTACAGGCACAAACA
GGTGCCTCTGGAAGGCCAGGCATCCATGACCCACTCTCATGACTGGGTCTGGCTGGGCAATGAGGCGGAG
CTCTTGGAGAACATTGGGGACCTGTCCCCACCCCAGGATGAGCACACGACCATCATAGACCGAGGGCTGG
GGGGCTGTGAGGAGAACAACCACTTACTTCTCAACGGTGGCTCCCAAAAGCCCACGCAGAGCCAGGTTCA
CAGGCCGCCAGGCTCCGGGGGACGGCAGACCAGGGAGCCCAGGCAGGAGCCTGCAAACTCACCCACCTCC
AAGATGAAGAAGGTCAAGTTTGCCACATTCACCATCCCACCTGAGGAAAGCTGCCCCACGGTGAACTCCA
TCCTCAGTGGGGAAGATGATATCAAGTGGGTTTGTCAAGACCTGGACGTGGGCGCACCCAAGGAACTCAG
AACCTACCTGGAGAAATTCCAAGACAGTGTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214981 representing NM_175432
Red=Cloning site Green=Tags(s)

MDGVTAATMRSEGAAPRRAARYGALSLVLATLLGQVTESRGVMDNIQRFSSLPPYLPVSFHVLRAETAFF
LKEANPDPLRNASLQSRVESFFIYKAQQPPVLNVSYGPYSAEKVIPLDLMLNPNFLGPTSKFPFDWRLKA
YILQEKVYLSHPKVQVLFHIVGRDWDDHRDEKLPCLRVFAFRDSREVRGSCRLGGPLGLCVAQLEMLPGW
FSPPAVVSGRRRPAERPEGSPVELYYAVQPGDERGDCTGGDTRKDNAIRPGKDGQEGRTSHLQKIGTISL
YRAQDSTQLSELRLDGNVVIWLPSQPVKQGDIVTASVTIANNSTVDHFILRAKVKKGVNILTVQTSEPRQ
WDVRQEVGNGGKHTTTSVACQRLGPGARNRSSNLFSEVMQMNFEIASFSSLSGTQPITWQVEYPRKGATD
IAVSEIFISQKDLVAIVPLAMDTELLNTAILTGKTVAMPVRVVSVEENSTLRDISELVECKATDENVIKV
SDHCDYVFVNGKEIKGKMDSVVNFTYQHLSAPLHVTVWVPRLPLQIEVSDTELSQVKGWRVPIVASKRPT
RDSEEEEEEEQKGRGCTLQFQHATVRVLTQFVSEGAGPWGQLSHLLSPDWQFDITHLVADFMKLESPHIA
TLQDSRVLVGREVGMTTIQVLSPLSDSILAEKTVTVLDDKVSVTDLAVQVVAGLSVTLHPISENNKATSA
VAMAEELLRAPKKEAIISTWLQFSDGSVTPLDIYDSKDFSLTAISLDEAVVSIPQPLSPWWPTVVAEGEG
QGPLLRVDMSIAEACQKSKRKSVLAVGIGHVGVKFGWDDADSSQTGEKDEEEIKNHASDRRQKIQDLERP
GQDELYHGNFPGDREEGALSATTTTKSLLDNNVGKSGRRDGARLHSIPIDFTNFPAHVDLPKAKTRGTLE
ENGLMQTAHGLSDLEIGMYALLGVFCLAILVFLINCATFAFKYRHKQVPLEGQASMTHSHDWVWLGNEAE
LLENIGDLSPPQDEHTTIIDRGLGGCEENNHLLLNGGSQKPTQSQVHRPPGSGGRQTREPRQEPANSPTS
KMKKVKFATFTIPPEESCPTVNSILSGEDDIKWVCQDLDVGAPKELRTYLEKFQDSV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175432
ORF Size 3321 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175432.3, NP_780641.2
RefSeq Size 5113 bp
RefSeq ORF 3324 bp
Locus ID 208213
UniProt ID Q8CEF9
Cytogenetics 5 G1.2
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Documents
Resources
"NM_175432" in other vectors (4)
SKU Description Size Price
MC223596 Tmem132c (untagged) - Mouse transmembrane protein 132C (Tmem132c), (10ug) 10 ug
$1,061.00
MR214981 Tmem132c (Myc-DDK-tagged) - Mouse transmembrane protein 132C (Tmem132c) 10 ug
$589.00 $964.00
MR214981L3 Lenti ORF clone of Tmem132c (Myc-DDK-tagged) - Mouse transmembrane protein 132C (Tmem132c) 10 ug
$1,264.00
MR214981L4 Lenti ORF clone of Tmem132c (mGFP-tagged) - Mouse transmembrane protein 132C (Tmem132c) 10 ug
$1,264.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.