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Fam187b (NM_175240) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214965
Fam187b (tGFP-tagged) - Mouse family with sequence similarity 187 member B (Fam187b), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $657.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fam187b
Synonyms 1700020B09Rik; Tmem162
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214965 representing NM_175240
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGCTACTCTGTGGCTGGTTGGTCTTTCTCTTCCCATGCTGTGGGCCCAGAGACTAATCAGCTGTC
CCTACAAGAATGTGTGTCAGTATGCCCTGCTCTCAGGCAGTGACGTGATACTGCAGTGTAACTACCCCAA
AGCACTTTGGTATTTTTCTTCATCTTTAGAGGACAAACTGTCCCTGGTCAACTCCAAGCCTGATGGGAGG
GTACTGCCTGGGAGCGACCTGCAGTTGTCTGACCCTAAGCCTTCCCAGACAGGCCTCTACCGCTGCCTGG
ATAATCACAAGGCCCGTTTGGTAGAATATGAGATTGACTTTCAGAACATAGCTCTGTTGCACATCACTCA
CAAAGACCTGGGCCAAGAGCCCATGGGAAATGAGAGCATGAACCTGGGAGGCAAGGTTCTTGTTTTTACC
CGCTGGGATCCTTGGCAAGATTGTAACCGTTGCCAAAAGCCAGGTGAGCGCAAACGTCTAGGCTACTGCT
ATGTGGAGGAGCCCCAAGAAAAGCCCATGCCTTGCTGGCTCTACCTGAGAGAGGAGAAGGTGACCAACAG
CCGCCTGAGGCCTGAACTCCAGCTACAAGCCTGCCAGGTACCCTGTGACACCGCCACGGAGACCAACCAG
CCGTACTTTGTCTTTGACACATATCTGCTGGACAAACCCAACAGCAATGCTCGACTCAAGTGTCCCTTGG
CTTCCATCTACAGGCCTGTCCACTGGGAAGCTGACAACAGCCCTCTGACATGGCAGGACCAGCTCTCTGG
CCAGACGGTCAGCACCATCATGGACCTGCACAGTGGTGGCCAGCACCTGAAGGTCTTCCAGCCTGCCACT
TACAGATGTTTTGTGGAGCAGGAGCTCATAGCCCAGTTCAACCCCACTGAGAGACAATCCAAAGCCCAGA
ACCCTTGGCAGCCCAGAATCCAGCCAGACAAGGCAGACTCCGTTCTTAGGAGATTGAAGTTGATGGTGCT
GAGTATCTCTGTACTGGCCGTTGGAGGGCTTCTCTGTAAGGTGGTTTTCCGTCCTGTCTGCGGCAAGAAG
AGGAGTCAGGTCCTCCTGGTGAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214965 representing NM_175240
Red=Cloning site Green=Tags(s)

MLATLWLVGLSLPMLWAQRLISCPYKNVCQYALLSGSDVILQCNYPKALWYFSSSLEDKLSLVNSKPDGR
VLPGSDLQLSDPKPSQTGLYRCLDNHKARLVEYEIDFQNIALLHITHKDLGQEPMGNESMNLGGKVLVFT
RWDPWQDCNRCQKPGERKRLGYCYVEEPQEKPMPCWLYLREEKVTNSRLRPELQLQACQVPCDTATETNQ
PYFVFDTYLLDKPNSNARLKCPLASIYRPVHWEADNSPLTWQDQLSGQTVSTIMDLHSGGQHLKVFQPAT
YRCFVEQELIAQFNPTERQSKAQNPWQPRIQPDKADSVLRRLKLMVLSISVLAVGGLLCKVVFRPVCGKK
RSQVLLVK

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175240
ORF Size 1074 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175240.5
RefSeq Size 1485 bp
RefSeq ORF 1077 bp
Locus ID 76415
UniProt ID Q0VAY3
Cytogenetics 7 B1
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Documents
Resources
"NM_175240" in other vectors (4)
SKU Description Size Price
MC211733 Fam187b (untagged) - Mouse family with sequence similarity 187, member B (Fam187b), (10ug) 10 ug
$503.00
MR214965 Fam187b (Myc-DDK-tagged) - Mouse family with sequence similarity 187, member B (Fam187b) 10 ug
$289.00 $457.00
MR214965L3 Lenti ORF clone of Fam187b (Myc-DDK-tagged) - Mouse family with sequence similarity 187, member B (Fam187b) 10 ug
$757.00
MR214965L4 Lenti ORF clone of Fam187b (mGFP-tagged) - Mouse family with sequence similarity 187, member B (Fam187b) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.