AU021092 (NM_001033220) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG214917
AU021092 (tGFP-tagged) - Mouse expressed sequence AU021092 (AU021092), (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol AU021092
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214917 representing NM_001033220
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAGACTCGGGCTGCTTCTCCTCCTGCTGCTGGCCCTGCCACCACACTTCTCCTCAGTGTCATGGC
CAGACACTGCACAGGGCACCATGGCAAACTTGATCCTGACTGCATTAGAAAAAGCCACCTTGTTCTTGGA
GGACAGGCTGCCCACAATCAACCTGGATGGTGTGGTGGGCTTCCAAGTGCTGGAAGTGCAACTCCGAGGA
GTTCAGGAAAAATGGGCTCACAAGCCCTTGCTGCAGCCTCTCAGCATGCGCGCTGGACAGATGGCCAACA
CACTGTCTGCTCTCCTCCAAAAATCCATCTTCTACCTCAAGCAGAGTGACCCCACGTACCTAAGAGAGTT
CCAGCCAAGCATTCAGCCTGGGTTTTGGAAGTTGCCCAATGACTGGACACGCACCAATGCCTCCCTAGTC
TACCCCTGGCTGGAACCCCTGGACTCTTTCTCAGAGGAAAGCAGCGATGTGTGCCTGGTGCAACTACTAG
GAACAGGGACAGACAGCAGCCAGCCTTGCAGGCTCTCCAACTTCTGCAGAACCCTTATGACCAAGGCCGG
CTGCTCAGGCTACAGCCTCTCCCATCAGCTGCTCTTCTTCCTCTGGGCCAGAATGCAAGGGTGCACGGAG
GGACTGTTCCTCCAGAGCCAACACTACATGGACATCTTCTGTGCCAATATGATGGAACTGAACCACAGAG
CTGAGGCCGTTGGATACGCTTACCCCACCCAAGACCTCTTCATGGAAAACATTATGTTCTGTGGTATGGC
TGGCTTCTCTGACTTCTACAAGCTGCGCTGGCTGGAGGCCATTCTCAGCTGGCAGAACCCCCAGGTGGGA
TGCTTCGGGAGGCCTGACACAAAGGGTGAACCTTCTGAAGTTCCACATCAGCAGGGCATTCTGAGAAGAG
TGCGAAGGCGGGAAAAACTGTTCGCAGATGGCTGTTCGTGCCACAACACAGCCACAGCAGTCGCAGCCCT
GGGTGGCTTTCTCTACATCCTGGCAGAATACCACCCAGACAATGGAGATGCACATCCAGAATACTACCCA
AACCATGGAGATCCATACTCATCCTCACAGTCACCAGCAAGCAACTACCAAGATGGTGCTGCCGGCCCTG
ACGTCCAGAGGACTGGCAGGCCCCTTAGTGTTTCT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214917 representing NM_001033220
Red=Cloning site Green=Tags(s)

MARLGLLLLLLLALPPHFSSVSWPDTAQGTMANLILTALEKATLFLEDRLPTINLDGVVGFQVLEVQLRG
VQEKWAHKPLLQPLSMRAGQMANTLSALLQKSIFYLKQSDPTYLREFQPSIQPGFWKLPNDWTRTNASLV
YPWLEPLDSFSEESSDVCLVQLLGTGTDSSQPCRLSNFCRTLMTKAGCSGYSLSHQLLFFLWARMQGCTE
GLFLQSQHYMDIFCANMMELNHRAEAVGYAYPTQDLFMENIMFCGMAGFSDFYKLRWLEAILSWQNPQVG
CFGRPDTKGEPSEVPHQQGILRRVRRREKLFADGCSCHNTATAVAALGGFLYILAEYHPDNGDAHPEYYP
NHGDPYSSSQSPASNYQDGAAGPDVQRTGRPLSVS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033220
ORF Size 1155 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033220.3, NP_001028392.2
RefSeq Size 1375 bp
RefSeq ORF 1158 bp
Locus ID 239691
UniProt ID Q3UST5
Cytogenetics 16 A1
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Documents
Resources
"NM_001033220" in other vectors (4)
SKU Description Size Price
MC213082 AU021092 (untagged) - Mouse expressed sequence AU021092 (AU021092), (10ug) 10 ug
$503.00
MR214917 AU021092 (Myc-DDK-tagged) - Mouse expressed sequence AU021092 (AU021092) 10 ug
$289.00 $457.00
MR214917L3 Lenti ORF clone of AU021092 (Myc-DDK-tagged) - Mouse expressed sequence AU021092 (AU021092) 10 ug
$757.00
MR214917L4 Lenti ORF clone of AU021092 (mGFP-tagged) - Mouse expressed sequence AU021092 (AU021092) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.