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Olfr310 (NM_001011520) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214899
Olfr310 (tGFP-tagged) - Mouse olfactory receptor 310 (Olfr310), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfr310
Synonyms MOR227-6P
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214899 representing NM_001011520
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAATTCCACCCTAGTGACTGAGTTCTTCCTGGAGGTTTTTGCTGAGATTTGGGAGCTCAGGATCT
TAATCACTGTGCTGTTCCTGCTGGTGTATCTGTGCAGCCTGTTAGGGAACCTTACCATCATCATTGTTAC
TACAGTTGACCAGACCCTGAACACACCCATGTACTTCTTCCTCAGGAATCTGTCCATTTTAGACATGGGC
TACATTTCTATCACTGTCCCCAGTACCTGTATCAACTCTCTCACTAACCACAGGAATATGTCTGTGGCTG
GGTGTGCAGCACAAATCTTTTCGTTCTTATTTTGTGCATGTGTAGAGATTCTAGTTTTTTCCATCATGGC
CCAAGACCGCTATGTGGCCATCTGCAAGCCTCTGCTCTATCCTGTGATCATGAACCACCAGTTCTGTGTT
CAGATGACACTGGCTTCCCTACTCAGCTCCCTTGTCATTGCAAGTGTGCACACTTTCAAAACTTTCCAGC
TGTCCTTCTGTCACTCTAATGTGGTCCCTCAGTTCTTCTGTGATCTTCCTGCTTTGCTGAGACTTTCTTG
CTCTGACACCTTTAGCAACAAACTCCTAATACTTCTGACTGTCATTGGAGTCAGTGGTAGTTGCTTTGTC
TTCATTGCCATATCATATATTCACATATTATCAACTGTATTGAAGATTCCCGTCAAAGGAGAGAGAGGGA
AAGCCTTTTCTACCTGTGTTCCTCACATTATTGTGGTGTCTGTCTTTGTTAGTTCTGCTGCCTTTGTGTA
TCTACGACCTCCAGTAATAACCTTTGAAGTAGTTCAGGAAATGGTTATTTCTGTATTTTATACCATGGTT
CCACCATTCTTAAACCCAATTGTCTATAGTCTTAGAAACAAACAGATAAAGGAGGCAGTGAGGAAAGTTA
TATTGCGAGTTTTTCCCATATTTGAATATAAAAGGAACATCTATTTCTCATGGTATTTTAGAGGGAAAAA
AACTGATAAAGTAAGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214899 representing NM_001011520
Red=Cloning site Green=Tags(s)

MANSTLVTEFFLEVFAEIWELRILITVLFLLVYLCSLLGNLTIIIVTTVDQTLNTPMYFFLRNLSILDMG
YISITVPSTCINSLTNHRNMSVAGCAAQIFSFLFCACVEILVFSIMAQDRYVAICKPLLYPVIMNHQFCV
QMTLASLLSSLVIASVHTFKTFQLSFCHSNVVPQFFCDLPALLRLSCSDTFSNKLLILLTVIGVSGSCFV
FIAISYIHILSTVLKIPVKGERGKAFSTCVPHIIVVSVFVSSAAFVYLRPPVITFEVVQEMVISVFYTMV
PPFLNPIVYSLRNKQIKEAVRKVILRVFPIFEYKRNIYFSWYFRGKKTDKVR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001011520
ORF Size 996 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001011520.2, NP_001011520.2
RefSeq Size 1082 bp
RefSeq ORF 999 bp
Locus ID 258222
Cytogenetics 7 D3
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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Resources
"NM_001011520" in other vectors (4)
SKU Description Size Price
MC213427 Olfr310 (untagged) - Mouse olfactory receptor 310 (Olfr310), (10ug) 10 ug
$330.00
MR214899 Olfr310 (Myc-DDK-tagged) - Mouse olfactory receptor 310 (Olfr310) 10 ug
$289.00 $300.00
MR214899L3 Lenti ORF clone of Olfr310 (Myc-DDK-tagged) - Mouse olfactory receptor 310 (Olfr310) 10 ug
$600.00
MR214899L4 Lenti ORF clone of Olfr310 (mGFP-tagged) - Mouse olfactory receptor 310 (Olfr310) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.