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Gm4301 (NM_001166637) Mouse Tagged ORF Clone

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SKU
MG214814
Gm4301 (tGFP-tagged) - Mouse predicted gene 4301 (Gm4301), (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
  $530.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gm4301
Synonyms 100043224
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214814 representing NM_001166637, codon optimized.
Due to the complexity of NM_001166637, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAACTATGGCTGATAAAATGGATATGAGTCTCGAAGACATTCTTAAGCTGAACAAGATGCAGCAGG
GTCGGCTGGATCGGCCCGACTCTCGGGTTAAGCGAGGAACAGGCCCAAAGCGCTACCGACCTGCTTTTAC
ACATGACGGCAGGAACAGATTGGCCCCTTATTGCCGCCCAAAACAGCTTCCTGATAAGTGGCAGCATGAC
CTCTTTATCGGTGGCTTCAGGGGACAGAACCACGTGGACACAGGAGGCAAGCTGTTTCTTAGCAACTTGC
ACTTTGGGGTGAGCGACGCTGACATCCAGCTGCTCTTCGCTGAATTTGGGACACTGAAGAAGAGTGCCGT
TCACTACGACAGGTGCGGTAGGTCACTGGGTACCGCCCACGTGCATTTTGAGAGAAAGGCAGATGCCCTG
AAGGCCATGAGAGAGTATAATGGTGCCCCTCTCGATGGAAGACCTATGAACATACACCTGGTGACATCCC
AGATAGACCGCCAGGGCAGGCCTGCCCTGAACTCAGACAAGGGTGGGATGACACGCAATCCAGGTTCAGG
GGTGCTGTCTGGCGGAGGCAATAAGCGATGGACACTTGGTGGCAGCCAAGGACGGGGACGCGGCACCAGC
CGAAATTCCAAGCTGCAGCAACAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAACAGCAAGAGG
AACAGAAACACCAGAAACAGCAGCAACAGAAGCAGCAGCAGCAACAACAGAAACAGGGCCAGAACCATCA
GCACCAGCAGCAGCAGAAGGAAAAAGAGCTGCAGCAACTGTCTGTCGAGGAACTTGACGCCCAGCTGGAC
GCTTACCAGGAGATGATGGACACTTCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214814 representing NM_001166637
Red=Cloning site Green=Tags(s)

MTTMADKMDMSLEDILKLNKMQQGRLDRPDSRVKRGTGPKRYRPAFTHDGRNRLAPYCRPKQLPDKWQHD
LFIGGFRGQNHVDTGGKLFLSNLHFGVSDADIQLLFAEFGTLKKSAVHYDRCGRSLGTAHVHFERKADAL
KAMREYNGAPLDGRPMNIHLVTSQIDRQGRPALNSDKGGMTRNPGSGVLSGGGNKRWTLGGSQGRGRGTS
RNSKLQQQQQQQQQQQQQQQQQEEQKHQKQQQQKQQQQQQKQGQNHQHQQQQKEKELQQLSVEELDAQLD
AYQEMMDTS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001166637
ORF Size 867 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001166637.1, NP_001160109.1
RefSeq Size 914 bp
RefSeq ORF 870 bp
Locus ID 100043224
Cytogenetics 10
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Documents
Resources
"NM_001166637" in other vectors (4)
SKU Description Size Price
MC215624 Gm4301 (untagged) - Mouse predicted gene 4301 (Gm4301), (10ug) 10 ug
$330.00
MR214814 Gm4301 (Myc-DDK-tagged) - Mouse predicted gene 4301 (Gm4301). Note: ORF is codon optimized 10 ug
$330.00
MR214814L3 Lenti ORF clone of Gm4301 (Myc-DDK-tagged) - Mouse predicted gene 4301 (Gm4301). Note: ORF is codon optimized 10 ug
$630.00
MR214814L4 Lenti ORF clone of Gm4301 (mGFP-tagged) - Mouse predicted gene 4301 (Gm4301). Note: ORF is codon optimized 10 ug
$630.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.