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D630003M21Rik (NM_177657) Mouse Tagged ORF Clone

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SKU
MG214801
D630003M21Rik (tGFP-tagged) - Mouse RIKEN cDNA D630003M21 gene (D630003M21Rik) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,234.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "D630003M21Rik or NM_177657"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol D630003M21Rik
Synonyms RP23-407H16.1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214801 representing NM_177657
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCTTCTGCCCTTGACATGGCCATCCAGCATGCCCTGGCAGGACTCTACCCGCCTTTCGAAGCCA
CTGCTCCCACTGTCCTGGGTCAAGTGTTCCGGCTCCTGGACTCCGACTTCAGGGGAGATGGACTGAGCTT
CCTCTTGGATTTTCTCATCCCTGCCAAGCGTCTGTGTGAGCAAGTGCGGGAAGCGGCTTGTGCCCTCTAT
ACCCACTGCCTCTTCCTGCATGAGGGCTGGCCGTTGTGTCTGCGGGATGAAGTTGTGGTACACCTGGCAC
CACTCAATCCACTCCTGCTACGTCAGGGTGATTTCTACCTTCAAGTAGAGTCCTGGGAGGAGCAGTCTGT
CCACATGACACTCAAGTGTCTCTCTTCAGACCTCCGTGAGGTGGACAAGAAGCCAATTCCTGAGTCCTCC
TACTCTCTCATTTTCACCCCAGAATGGTTGGAGGCCATCAACAATGACTTTGAGGGACGTCCCTTACACA
ACTGTCTCGTGGCTTCAGAAAATGGGATCACACCTGTGCCTTGGACCAAGATTACCAGTCCAGAGTTTGT
AGATGACAGACCCCCAATAGTGAAGGTCCCTTCCTCAGACGGGGACTCCTGTCCACTAGAAGACCTCCAT
CTGAGCAGACCCCAGGAGCCATACCAGGCTGGTGACTTGGGCGGCAAGGGATCTGTGGCCCAGATCTGGG
ATAAGGGTAAAGGGAAGTTGTCTGGGGACAAGTATCCAGGGCTCATCAAGGTGGAGCCAGCAAGGTCAGG
TCAGCTCGCCTTTAGGACAGACAGTGAGGCCAGCCAGAGCCTGGAGGGGGACTATGTGGCTCTCCTGGGC
TTTCCCCAAGAGTATAGAGGGGCATCCCCAGACAGTGAGGTAGTGACATTAAGTGTAGATATTCAAAAAT
CACAGGAGACAAGACCCAGAACTAAGGGGGCACCTCTGCTTGGAAAGACCCTTCCTCTCTCGGGGCCTGC
TGGGGCACCTCCCTTGGGAAGATGGGCATGTGAAAGGCCAGCAGGCTCAGGGGAGAAGCCCTGTAGCGGA
GGCTCAAGGAGAAAGGCCAGGCATAAAGCATCTGGCCACACTGCAGTGCGACAGCCACAGCAGCCCCATA
CTAGTGTCCCGGAGAAGCTGCCGGACTGTACCTCTGGCCTGGTGGAAGACACTGAGGAAGAACCAGCAGC
CTCTGAGATGCAAAAGCCTGTGGGAATGCCAGAAGCCATGGTTCGGCTCAGGCCTGGGCCAAGACAAGCA
TTCTCTCCCCTCCTGTCTTCAGCTGGCCCTGGATCCCCAGCGGCTGAGACAAAGACAGAGGAGACCACAC
TAGGACATGGGAGAGCTTCCAAGCCTGAGGACTGCCTCAATAAGAACGCCTCCTTCCATGGGTCCCCCGC
TCCTGGGCTCCAGTTCTCCTTCTTGAAAGAACAGAGGGTACCCCATGTGACCCCCGAGAAGGCCCTACTC
CAGCATGCCAGGCCTCGGAAGGCCCTGTGCCCCCTCTACTCTCTGCAGCCTTGTGAAGCCAAGGCCCCAG
GCAAAGATGGAACAACTCTCCCCACAACATCTGGCTCAGGCCCACTGTCTGGGGAGCCGCCTGGCTTCCG
GAGAGATTCTGCAGGTCCTCCAGGGGGAGGACTTCGCCTGGAGGAGTGTTCTAGGACTAAGCCCAGGATT
GAGACCCTGGGAGTCAAGCTTCTCCAGTCCGGGATAGCCTGCCTGCCAGGTGGTAGGGACAAGGTGGGGA
GACCGCTGCTTCTGGTGTCAACCGCAGAGGATGCCTGGGAGGCACCATGGTGTACTACCTCAGAGGTCGC
GGAGTTGCTGTCCTACTTATGCAGTGTCCCTAGGCTGGAAGATAAAGCCAAGGGGCTCCTGGTGGTGATT
GATGCCAGGAAAGGGACCCAGAGGCCTGGTCTGGTCAGCGCCCTGCAGGGCATCCAGGCTCTGGCTCCGG
CCTCCGTCAGTAAAGTGCTCCTCCTCGGAGAGAAGGCGTCCATTCCCCAGCTATCTGTGCTACCTGCCCA
GGTGGAGGTACTGACGTCGCTGAAGGCCCTCAGAAACCACGTGGACCCTAGCCAGCTGCCGGAGGCTCTG
GAGGGCCCCTTTCCCTACCACCACAGCGAGTGGGTGCAGTTCTTCCAGAAACTGGACCCATTCCTCACTG
ACCTTCGCCAGGCCTCCTCCCTGCTGCAGGCTTCCATCCAAGAGTTTGAGAAGGGTGAACCCCCTGGTGG
GGTGCAGGAGGCTACCAGGTGCCTGAGCAAGTCCAAGGAGCTGATGGAGACGGTGCTGAGGGACCCGGGC
CTGCTGGCTCTCCAGAGGGAAGGTGGAACTACTCTGGCCAGCTTGCAGCAAGAGGCCAGCGGGCTAAATG
CTAACCCCGATGTCAGGAGCCATCTGACCGAGGCTGCTGCCCTGTACAACCTTGTGGATGGTCAGCTTCA
TGACTTGGTCACTGCGTCCAATCAACTCCTCCGAAGGCTGGAGCTCCGCGTCCGCCTGGGCCACCTGGAG
ACTGCCATCCACCAGGTCAGTGACTGGATGGCGGGAGAAGGCAGCCAGAGTCTGCAGGCTCTCGCTCCTG
TGCATGTATGTGCAGAGACAGTAGAGAAAGTCCACGCAGAGTTTGAGGATTTCTTCCTGCAGGTTGCGGC
CCAGTACCGCCAGGGCCTTGACCTGTCCAAGCAGGCGGCCCAGCTGGGAGCTGCAGAGGAAGGGGCAGGT
GAGATGGGGCTCCCAGACCTGGCGGCCTTCGCCTCCACCCAGCAGGCCTTCCAAGCCAGGCTGACACACT
TCTACATGGCCGCTGAGAGGCAGCGCACTGACCTGGAGACCCTGCTCCATCTCCACCGTTTCAGAAGGAA
GATGTCCCGGTTCCACATGGACTGCCAGAACCTGCTGACCCAGCTCAGCCTGGGCAAGGCTGTGAAGGCC
AGCCCTGGGGACCAGTTGCACCTCCGACTCCACTGCTACCTGAAGCGCCTGGCTTCTGAGTTCCAGACGG
AGAAACTCCTGGCCATGAAGCTCCAGGTGGCCTCCCTGAGCCGGCCTGGCCTGGGCCAGGAGGTGTGGGA
AGAGGCCCAGGAGCGGCACCAGGAGATCCAGAGCTTGCTGAGGAAGGCACTAGCCTACTGCCCATGCCCA
GAGGTTCCAGCTACCCAAGTGGCACTCATAGACCGAAGCAGGCCAGTGGCTAAGGGTCAGGGTCTGCCTA
GAGAAGTGGGTTCCAAATGGGACAGGTCTCTACAGGACTCTCTGGCTGTGGATCATGTGTTCAAATCCCA
GCGGACCCCTCAAGGGGAGCAGAGCAGAAACATGTGGGCAGGCCTGCTATCCCCAGAACCTGGCCAGTCT
GGGGACACTGAGGAAGTCAGGGGCACCCCCAAGCTCCCCGATCCCACCCTGGAGCGGCTCCTTGCCTCCC
TCTTCTCCTGGCCCCACCTTCCCAAGCAGAGCAAGGCCTCCCGTCCAACTGGGGGAAGCTTTTCCTCAGA
AGGGACAGGATCACAGACATCCCTGGAGGACTCACCCCACACAAGTCCTCCTGCGTCCCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214801 representing NM_177657
Red=Cloning site Green=Tags(s)

MDPSALDMAIQHALAGLYPPFEATAPTVLGQVFRLLDSDFRGDGLSFLLDFLIPAKRLCEQVREAACALY
THCLFLHEGWPLCLRDEVVVHLAPLNPLLLRQGDFYLQVESWEEQSVHMTLKCLSSDLREVDKKPIPESS
YSLIFTPEWLEAINNDFEGRPLHNCLVASENGITPVPWTKITSPEFVDDRPPIVKVPSSDGDSCPLEDLH
LSRPQEPYQAGDLGGKGSVAQIWDKGKGKLSGDKYPGLIKVEPARSGQLAFRTDSEASQSLEGDYVALLG
FPQEYRGASPDSEVVTLSVDIQKSQETRPRTKGAPLLGKTLPLSGPAGAPPLGRWACERPAGSGEKPCSG
GSRRKARHKASGHTAVRQPQQPHTSVPEKLPDCTSGLVEDTEEEPAASEMQKPVGMPEAMVRLRPGPRQA
FSPLLSSAGPGSPAAETKTEETTLGHGRASKPEDCLNKNASFHGSPAPGLQFSFLKEQRVPHVTPEKALL
QHARPRKALCPLYSLQPCEAKAPGKDGTTLPTTSGSGPLSGEPPGFRRDSAGPPGGGLRLEECSRTKPRI
ETLGVKLLQSGIACLPGGRDKVGRPLLLVSTAEDAWEAPWCTTSEVAELLSYLCSVPRLEDKAKGLLVVI
DARKGTQRPGLVSALQGIQALAPASVSKVLLLGEKASIPQLSVLPAQVEVLTSLKALRNHVDPSQLPEAL
EGPFPYHHSEWVQFFQKLDPFLTDLRQASSLLQASIQEFEKGEPPGGVQEATRCLSKSKELMETVLRDPG
LLALQREGGTTLASLQQEASGLNANPDVRSHLTEAAALYNLVDGQLHDLVTASNQLLRRLELRVRLGHLE
TAIHQVSDWMAGEGSQSLQALAPVHVCAETVEKVHAEFEDFFLQVAAQYRQGLDLSKQAAQLGAAEEGAG
EMGLPDLAAFASTQQAFQARLTHFYMAAERQRTDLETLLHLHRFRRKMSRFHMDCQNLLTQLSLGKAVKA
SPGDQLHLRLHCYLKRLASEFQTEKLLAMKLQVASLSRPGLGQEVWEEAQERHQEIQSLLRKALAYCPCP
EVPATQVALIDRSRPVAKGQGLPREVGSKWDRSLQDSLAVDHVFKSQRTPQGEQSRNMWAGLLSPEPGQS
GDTEEVRGTPKLPDPTLERLLASLFSWPHLPKQSKASRPTGGSFSSEGTGSQTSLEDSPHTSPPASL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177657
ORF Size 3561 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177657.4, NP_808325.2
RefSeq Size 3941 bp
RefSeq ORF 3564 bp
Locus ID 228846
UniProt ID Q8BWG4
Cytogenetics 2 H1
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Documents
Resources
"NM_177657" in other vectors (4)
SKU Description Size Price
MC223899 D630003M21Rik (untagged) - Mouse RIKEN cDNA D630003M21 gene (D630003M21Rik), transcript variant 1, (10ug) 10 ug
$1,137.00
MR214801 D630003M21Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA D630003M21 gene (D630003M21Rik), transcript variant 1 10 ug
$589.00 $1,034.00
MR214801L3 Lenti ORF clone of D630003M21Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA D630003M21 gene (D630003M21Rik), transcript variant 1 10 ug
$1,334.00
MR214801L4 Lenti ORF clone of D630003M21Rik (mGFP-tagged) - Mouse RIKEN cDNA D630003M21 gene (D630003M21Rik), transcript variant 1 10 ug
$1,334.00

file-alt Citations

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