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BC005561 (NM_001166581) Mouse Tagged ORF Clone

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SKU
MG214787
BC005561 (tGFP-tagged) - Mouse cDNA sequence BC005561 (BC005561), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,559.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

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PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
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Other products for "BC005561 or NM_001166581"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol BC005561
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214787 representing NM_001166581
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGATGACCTCGACAACTGTAGTGGTTCCTGCAGAGTGGATCAAGACCTGGGAAAAATCTGGGAGAG
GCGAGTTTTTGCGTTTGTGCCGGATTCTCAGTGAAAGTAAAAGCCGAGACACTTTTGCTTGGAGAGATTT
CCAACAAGCACTCTATGAGTTGTCATACCATGTCATTAAAGGAACTTTAAAGCCCGAACAGGCCTCAAAT
GTTCTTAATGACATCAGTGAATTTCGAGAAGATATTCCATTTATTCTTGCTGATATATTCTGCATATTAG
ATATTGAGACGAATTGTCTAGAAGAAAAAAGCAAAAGAGATCATTTTACACAACTGATATTAGCATGTTT
ATATTTAGTCTCAGACACAGTTCTAAAGGAACGCTTGGATCCAGAAACATTGGGGTCATTGGGCCTTATC
AAACAATCGCAGCAGTTCAATCAAAAATCAGTTAAGATCAAAACGAAACTCTTCTATAAACAGCAGAAAT
TCAATTTGCTAAGAGAAGAAAATGAAGGTTATGCCAAGCTGATTGTTGAATTAGGGCAAGATTTATCTGG
AAATATCACTAGTGATTTAATCTTAGAAATTCTCAAATCTTTAATAGGGTGCTTTAATCTAGATCCCAAT
AGAGTTTTGGATATCATTTTAGAAGTGTTTGAATGCAGGCCAGAACACCATGATTTCTTTATATCTTTAT
TAGAAGCTTATATGAGTATGTGTGAACCACACACACTGTGTCATATTCTTGGGTTCAAATTCAAGTTTTA
CCAGGAGCCAAATGGTGAGACCCCATCTTCCTTGTATAGAGCTGCAGCAGTGCTCCTGCAGTTTAACCTT
ATCGATTTGGATGATCTCTATGTGCACCTTCTTCCAGCTGATAGCTGCATTGTGAGTGAATACAAACGGG
AAATTGTGGAAGCTAAGCAAATTGTTAAAAAACTTACAATGGTTGTATTGCCTTCCGAAAAAAGTGATGA
ACGAGAAAAAGAAAAGAAAAAAGATGATAAGGTAGAGAAGGCACCTGACAACCAAAAACTTGGTTTGTTG
GAAGCCTTGTTAATTATTGGTGATTGGAAACATGCACAAAGTATTATGGACCATATGCCTCCATACTATG
CAACATCACATAAAGTAATAGCCCTTGCTATTTGCAATCTTATACACATAACTATTGAGCCTATTTACCG
AAGAGTTGGTGTCCCTAAAGGTGCCAAAGGCTCACCTGTCAATGCTTTACAAAACAAGAAGGCACCAAAG
CAAGCAGAGAGCTTTGAAGACTTAAGGAGAGATGTATTCAGTATGTTCTATTACCTTGGTCCTCACCTTT
CTCATGATCCCATCTTATTTGCAAAGGTTGTGCGCATAGGCAAGTCATTTATGAAGGAATTTCAGTCTGA
TGGGAAACAAGAAAATAAAGAGAAAATGGAAGCTATTCTTAGTTGTTTGCTTAGCGTTACTGACCAGGTA
CTGCTTCCTTCTCTTTCTTTAATGGATTGCAATGCGTGCATGTCTGAGGAACTATGGGGAATGTTTAAAA
CATTTCCTTATCAACATAGATATCGCTTATATGGACAGTGGAAGAATGAGACTTACAACAGCCATCCACT
TTTAGTGAAAGTTAAAGCTTATACAATAGATCGAGCCAAATACATCATGAAGCGCCTAACAAAGGAAAAT
GTGAAGCCTTCAGGAAGACAGATTGGGAAATTGAGCCACAGTAATCCAACCATTTTGTTTGATTATATCT
TGTCACAAATACAGAAGTATGATAACTTAATAACCCCTGTGGTAGACTCATTGAAATACCTCACTGCGTT
GAATTACGATGTTTTAGCCTATTGTATCATTGAAGCACTAGCTAATCCAGAAAAAGAAAGGATGAAACAT
GATGACACAACCATCTCCAACTGGCTTCAGAGTCTGGCTAGTTTCTGTGGTGCAGTTTTTCGTAAATACC
CAATTGATCTTGCTGGTCTTCTTCAGTATGTAGCCAATCAGTTAAAGGCAGGCAAAAGTTTTGATCTACT
TATATTGAAAGAAGTAGTACAAAAAATGGCAGGAATAGAAGTTACAGAGGAAATGACAATGGAGCAGCTT
GAGGCTATGACAGGTGGGGAACAACTGAAAGCTGAAGGTGGATATTTTGGCCAGATTAGAAACACTAAAA
AATCCTCTCAGAGATTAAAGGATGCACTACTGGACCATGATCTTGCCCTTCCTCTTTGTTTGCTCATGGC
ACAGCAAAGGAATGGGGTGATATTTCAGGAAGGCGGAGAGAAACATTTGAAACTTGTGGGAAAGCTATAT
GACCAGTGTCATGATACCTTGGTACAGTTTGGTGGGTTTTTAGCATCAAATCTAAGCACAGATGATTACA
TAAAGAGAGTTCCTTCAATTGATGTGCTTTGTAATGAATTTCATACACCCCATGATGCAGCATTTTTTCT
GTCTCGGCCAATGTATGCACACCATATTTCATCAAAGTATGATGAACTTAAGAAATCAGAAAAGGGTAGT
AAGCAGCAACATAAAGTTCATAAGTACATTATGTCATGTGAGATGGTGATGGCTCCTGTCCATGAAGCAG
TGGTCTCGTTACATATCTCCAAAGTCTGGGACGATATCAGCCCTCAGTTCTATACCACATTCTGGTCTCT
GACCATGTATGACCTTGCAGTGCCACATACCAGCTATGAACGGGAAGTCAATAAACTGAAGATCCAAATG
AAAGCAGTTGATGACAGTCAAGAAATGCCTCTAAATAAAAAGAAAAAGGAGAAAGAGCGCTGCACTGCCT
TGCAGGACAAGCTTCTTGAAGAGGAGAAGAAACAGACGGAGCATGTACAGCGAGTTCTACACAGGCTGAA
ATTAGAAAAGGACAACTGGCTCTTAGCAAAATCTACTAAAAATGAGACCATCACAAAATTTCTCCAGTTG
TGTATATTTCCTCGATGTATTTTTTCAGCAATTGATTCTGTTTACTGTGCTCATTTTGTTGAGTTAGTCC
ATCAACAGAAGACTCCAAATTTTTCCACACTTCTTTGCTATGACCGAGTTTTTTCAGACATAATTTACAT
GGTTGCTAGCTTTACTGAAAATGAAGCTAGTCGATATGGGAGATTTCTTTGCTGCATGTTAGAGACTGTG
ACTAGGTGGCACAGCGACAGATCTACCTATGAGAAGGAATGTGGAAACTATCCAGGATTCCTTACTATAT
TACGAGCAACTGGATTTGATGGTGGAAATAAAGCTGATCAGCTAGATTATGAGAATTTTCGACATGTTGT
ACATAAATGGCATTACAAACTAACCAAGGCATCGGTACATTGCCTCGAAACAGGTGAATATACACATATC
AGGAATATCTTGATTGTACTAACAAAAATACTACCATGGTACCCCAAGGTTTTAAATCTGGGTCAGGCTT
TGGAAAGAAGAGTGCATAAAATCTGCCAAGAAGAAAAAGAAAAGAAACCAGATCTATATGCATTAGCAAT
GGTCTACTCTGGGCAGCTGAAAAGTAGAAAGTCATACATGATACCTGAAAATGAATTTCATCACAAAGAC
CCTCCTCCAAGAAATACTGCTACCAATCTACAACCAAGTGGGCCTTGTAGTGGGCTGCCTTCATCTATAG
GAAGTATGTGTAAATTAGATGAGAGCAGTGCTGAGGAGGCCGACAAATCACGAGAAAGAGCTCAGTGTGC
TGTGAAAGCTGCTAATAAAGCTTCCAGTGTCACACCAAAAGGGAATTTAAGCAATGGAAACAGTGGCTCT
AACAGCAAAGCTGTTAAGGAAAATGACAAAGAAAAGGGCAAAGAGAAAGAGAAAGAAAAAAAAGAAAAGA
CCCCAGCTGTTACTCCAGAGGCCAGGGTACTTGGTAAAGACAGTAAAGAAAAACCCAAGGAAGAACAACC
AAATAAAGATGAAAAAATAAGAGAAGCCAAAGAAAGAATGCCTAAATCTGATAAAGACAAGGAAAAATTA
AAGAAGGAAGAAAAAGCTAAAGATGAGAAATTCAGGATCATTGTTGCCAATGTAGAATCAAAATCCACTC
AAGAAAGGGAAAAAGAGAAAGAGCCCTCAAAAGAAAGAGATTTAGCAAAGGAAATGAAGTCAAAAGAGAA
TGTTAAAGGAGGGGAAAAAGCACCAGTTTCTGGCTCCTTGAAATCACCTATTTCCCGAACAGATATCACA
GAACCTGAAAGAGAAAAACGTCGCAAAGTTGATTCCCATCCTTCTCCATCACACTCTTCCACAATAAAGG
ACAGTCTTGTCAAACTTAAGGAATCTTCAGCAAAGCTCTATATCAACCATATTCCACCACTACTGTGCAA
GAGTAAAGAGAGAGAAGCAGACAAGAAAGATTTGGACAAGTCAAGGGAAAGATCCAGAGAAAGAGAGAAA
AAAGAGGAAAAGGACAGGAAAGAGCGGAAAAGAGATTATTCAAACAATGACCGAGAGGCACCCTTGGACT
TAATCAAGAGGCGGAAAGATGAAAATGGAATATTGGGGGTTTCAAAACACAAAAGTGAAAGTCCCTGTGA
GTCTCTTTATCCAAATGAGAAAGACAAGGAAAAAATGAAGTCAAAATCCTCAGGCAAAGAAAAAGGTGAT
TCATTTAAACCTGAAAAGATAGATAAAATATCCAGTGGGAAAAAGGAGTCCGGACATGATAAGGAAAAGA
TAGAGAAGAAAGAGAAATGGGATGGTTCTGGAGATAAGGAAGAGAAGAAACATCATAAAACCTCAGACAA
GCACAGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214787 representing NM_001166581
Red=Cloning site Green=Tags(s)

MAMTSTTVVVPAEWIKTWEKSGRGEFLRLCRILSESKSRDTFAWRDFQQALYELSYHVIKGTLKPEQASN
VLNDISEFREDIPFILADIFCILDIETNCLEEKSKRDHFTQLILACLYLVSDTVLKERLDPETLGSLGLI
KQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIVELGQDLSGNITSDLILEILKSLIGCFNLDPN
RVLDIILEVFECRPEHHDFFISLLEAYMSMCEPHTLCHILGFKFKFYQEPNGETPSSLYRAAAVLLQFNL
IDLDDLYVHLLPADSCIVSEYKREIVEAKQIVKKLTMVVLPSEKSDEREKEKKKDDKVEKAPDNQKLGLL
EALLIIGDWKHAQSIMDHMPPYYATSHKVIALAICNLIHITIEPIYRRVGVPKGAKGSPVNALQNKKAPK
QAESFEDLRRDVFSMFYYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGKQENKEKMEAILSCLLSVTDQV
LLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAYTIDRAKYIMKRLTKEN
VKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTALNYDVLAYCIIEALANPEKERMKH
DDTTISNWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEVTEEMTMEQL
EAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLY
DQCHDTLVQFGGFLASNLSTDDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGS
KQQHKVHKYIMSCEMVMAPVHEAVVSLHISKVWDDISPQFYTTFWSLTMYDLAVPHTSYEREVNKLKIQM
KAVDDSQEMPLNKKKKEKERCTALQDKLLEEEKKQTEHVQRVLHRLKLEKDNWLLAKSTKNETITKFLQL
CIFPRCIFSAIDSVYCAHFVELVHQQKTPNFSTLLCYDRVFSDIIYMVASFTENEASRYGRFLCCMLETV
TRWHSDRSTYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHI
RNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKKPDLYALAMVYSGQLKSRKSYMIPENEFHHKD
PPPRNTATNLQPSGPCSGLPSSIGSMCKLDESSAEEADKSRERAQCAVKAANKASSVTPKGNLSNGNSGS
NSKAVKENDKEKGKEKEKEKKEKTPAVTPEARVLGKDSKEKPKEEQPNKDEKIREAKERMPKSDKDKEKL
KKEEKAKDEKFRIIVANVESKSTQEREKEKEPSKERDLAKEMKSKENVKGGEKAPVSGSLKSPISRTDIT
EPEREKRRKVDSHPSPSHSSTIKDSLVKLKESSAKLYINHIPPLLCKSKEREADKKDLDKSRERSREREK
KEEKDRKERKRDYSNNDREAPLDLIKRRKDENGILGVSKHKSESPCESLYPNEKDKEKMKSKSSGKEKGD
SFKPEKIDKISSGKKESGHDKEKIEKKEKWDGSGDKEEKKHHKTSDKHR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001166581
ORF Size 4767 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001166581.2
RefSeq Size 5181 bp
RefSeq ORF 4770 bp
Locus ID 100042165
Cytogenetics 5 E5
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Documents
Resources
"NM_001166581" in other vectors (4)
SKU Description Size Price
MC224730 BC005561 (untagged) - Mouse cDNA sequence BC005561 (BC005561), (10ug) 10 ug
$1,496.00
MR214787 BC005561 (Myc-DDK-tagged) - Mouse cDNA sequence BC005561 (BC005561) 10 ug
$589.00 $1,359.00
MR214787L3 Lenti ORF clone of BC005561 (Myc-DDK-tagged) - Mouse cDNA sequence BC005561 (BC005561) 10 ug
$1,659.00
MR214787L4 Lenti ORF clone of BC005561 (mGFP-tagged) - Mouse cDNA sequence BC005561 (BC005561) 10 ug
$1,659.00

file-alt Citations

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