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4930407I10Rik (NM_001166475) Mouse Tagged ORF Clone

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SKU
MG214777
4930407I10Rik (tGFP-tagged) - Mouse RIKEN cDNA 4930407I10 gene (4930407I10Rik), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,623.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "4930407I10Rik or NM_001166475"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 4930407I10Rik
Synonyms Gm747
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214777 representing NM_001166475
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGTTACCACTTCTGGGAGCCTGTGCTGTGATAGGTCCATTCCAGGGCCCTGAATGGGAGCCAGTGA
GGGGCCTCCTGTCCCAGGATCAAAGCTGCAAGGATCCCCGGTGCTGTGGCAATCTGCTTGTCTCCTGCCT
CTTTCTGATCTGGCAGATCCAACAGTATTGGCACCAGTTCTCCAGGACCAGAAAGAGGAATGTCACCAAG
GTGCCACCACAGAGATGGACAGTGTTGTCTGCCCCCCAGGACACTTTTGTTGGGATGATTCCTGAATTCT
TTACTCATGGAAAAAACAGGGGCCTGGATGTTCACATCCAACAGTGGATGCAGAAGCGGCGATGGGGATA
CCAGAAGACTGTCAGACAACAGTGGGACACTCAGTATCTGCTGTCTCTGCAGAAGCCATGTCAAGACCTG
CCTTGGGATGTCCATACCTCTACTGAGCCCATTTTTTGTACCTCTTCCTTTTCAAACACTTGTCTTCTCC
CTCAGGACAGTTCTTGGGAAGCATGGCAGTTACCCTGGTACCCCAGAGATAGCCAGGCCCACCCATCCCT
GGCTATATGCCAAAGAACGGAGCAGCTGCTGGATCCTTCTCATACGTTGGTGCCAGCAGAGCCTGTTAGC
CTGAGGTACACCTCCACAACTTTAGCCTTCTCTCTTCCAAACTTCCCTCCAGCTCAGAGTCCAAACGTCT
GCCTCAGAGAGTTTCTACCCAGTCCTCTTAACCAACAATTGGAAATGTCCACCAAGGAGTGCTTGGAGCA
CCCACAAGGTCCCTTAGGACCATGGAGTCAAACCAAGACAGTAGGCAGAGAATGCAGTGAAGCTACTCTG
CAATATCTAAACAAGAGAAAGAGCAGGAGGGAAGATGCTCAGGAGATACGAGCATCTAGAGCCTTCTGCC
CAATAGACTCTGGAGTGGAGGAGGAGGAACAGGCTAAAGCCCTGGGGTACAGAGACCAGAGGCAAGAAAG
AAGGGACACTGATGGCGAGATCTCAGTGTCAGAGTGGGAGAGGCAAACCCAGGTGAGGACTGCAGTGAGA
GAACAAACTGAGAAACTACAGAGGAAAGCCCAGAGGGAGCCTGGAGAGGAGAGCTCTTCTCCCCAGTCTC
ACATAGGAGAGAACCAAGAAGAGTTCAGATGTAAAACTGACATAGCAACTCAGATACCAGCGTGGGTCGA
CCAGGAGGATGCTGTGGAGACTCCAACACTGGGGAAGAATGAGAAAGAAGCCAGAGGGAAGGAAGAGGCT
GAGGTCGAGGCCCAAGGATTGGAAACTCAGGACTGGACGGGAAGTAAGGCTGCTGAGAATTCCCAAGGGC
TAAAGTGGGGGACACGAGACCAGTTTGGAGGCAATACTGGTACAGAAACTGAGGTGGAGGAAGGAAGGAA
CAAGGATCAGATCGGAAGTGGAGATGGTGTGAAAATCGAGACATCTGGAAGGGAGAATCCAGGAGAATTC
AAACAACAGGACAATGAAAAGACCCAGGCCCTGGGGTGGGAGAAACAGAGATGCATTAGAACTGAGAATG
ACGTCCAGACTCCAGCTGGGGAGCGTGGAGGACAGAGCAGCAGTGAGAACGACTGGAAGATCCAAGCATC
TAAGGGAGAGAACCAGAATCTGTCAAGACAGGAAGTTGAACTGGGCTTGAAGAAGCTCAGAGAGGTAAGA
GAAGAGGACTGGGTGGTGATCCAGGCGCCATGCTGGGGCAGCCAGAGTCTCAGGCTGATCGCAGTGAAAA
GAGGACCATGCTGCAGAGACCAGAGCCAGGTCAGAGGAGAATATACTGTAGACATCTCGACACTGGAAAG
TGACCAAAGAGAGGGTGGAGATGCTGATGCAGCAAACCAGGTGACACCCGAGGCAGAGGAGCAGGCTCGA
TCCAGTGAACCTGACATGGAGACTTGCATAGTACCGAGCCAGGATGAGGAAAAGACTGAAGAGGAAAATG
GAATGTATGCTCTGGCACAAGGGAAGAGAAACCTGAGAGGGGCTAACGGCACAGATGGAGCACAGACCCA
GAAACTTGGGGAAGAAAACCAGGGTCAGTTAGGAAATGAATCCCATAAAATGATTCACGGACCAAAACGG
AAGAATCAGAAACAGGTTAGAGGCAACGATCATATAAATAACCAGACATCTGAGACAGGGAACTGGGCGG
AGCTAACAAGCAAGAAAGGTGATATGACCCACGGGACAGGGTACGAGGAAGCTGAGGAGGCTGAGGGTGA
GGACGGCACAGAAGGAATGAGAATCGTGAGAGGAGCCGGAGGGGAGGAAGGTACAGAGACCAAGGCAGCC
GTGGAAGAAAGCCAGAGCCCGTTAGGTGATGTGGGTACAAACACCCATTCATCCGAATTAGAGAACCAGA
AGGAGATGGGCAGCGAGGATGCAACAGAAGCTCGGGCCCCGGAGCAGAGAAACAAGACAGAACCCGGAAA
TAATGACATAAAGACCCAGAGACCTGAGAGCGAGAGCCAGGGACAGCTGGCTGAAGCTGGAGGGATCGGG
GAACAGGCTAAAGGCGAGAGCACTTCAGGAAATGGGGCAGCGGAGAAGAAAAACTGGGGAGAGGGTGCAG
GAGAGGATTGTAGAAAGCTGCAGCGGCCCAGGAGAAAGGATCAGAGACTAAGGTTAGGTGAAGTTGATGG
AAAGACCTACCAGTTAGAGTGGAAGAATCAGGAGAACTTTAGAGATGGGAATGATGCCGACATTCAAAAA
CAAGGGAAGAGAAACCCACTATGTTTCACGGGAGACGATGGCTCAGACACCCGAGCCTCTGTGGGAGAGG
ACCAAAGACAGTCTGTGTGTGAAACGGATGAAAAGAGTCAGACACCGGGGCAAAGAAACCAGGGCAAGCG
TAGAGACACTGCTACAGAAATCCAGGATGTCGGGGTCCAGAGAAAACGCAGAGCTGAGGGCTCTAAACTG
TCTCATCCATCGGGGAGAGGAGACAAGGGCCGGGTTGGCAGGAAGGATGCTGTCAGGCCCAGTCCCCCAA
ATGACTCTTCTGGGAAAGTGGGGCCCACAAGGCAGAAGTGCAGCTCGGCTCAGCTGGCTTCTCTCACTTC
CGGATATGGAACCCCCGGACACAAGCAACCAATGGCTGGAAATGGTGTCGACTCTGCTCCCGGTTCTGAA
GAACATCTAAGTTGCCAGGGCAGAGCTGCTGCCTGGAAGCACAGGCGTGAAGTCAGTGAAAGAACCCAGA
AGGCCCAACCTGGATCTCAAAGAAGACAAGAAAGGGACAAAAGGGTGGACCTAGGGAAGGCTTCCAGCCC
GACCTGTCAGGACCCCTATCCACAGTCTCAGGCATCCTCTGTCTTTCCCTCTCTCCTCTGTCCCCAAGTC
TCTCAAGCTGCCCCAGCTGTACCATGCGTACCAGTAGCTCTCAAAACCCTTCACAAATGGCCAGCTCTCA
AGAAGAGCAAGCATCTGCTCCTGGAATCGCTCATGAAGAGGAGGATTGCACATCTGAGGTGGGGCCTCCC
TCGACGCATCCTGGAGTCGTACTTGCTTTTTCACTTTTTAGAATCTTGCTCCTTGCCGCCGGCTGGAGGG
AGGCTGCCTGGATCACGCACGAACCGGGAACGCCAAAGGCATCAGGAACGACATTGTGAGTCTCAGGCAT
CCCTGTTAGGTCTTGAGTCTCCAGTCGGGACTCAAAGCCGTCCAGTTCTTGAAAAAAAAAGTTCGAAACT
TTGTACCCAAGTCCAGGCTGTAGAGAAGCGTAGACCAACCAAGCCTGAGCCAACGGGCAGCTCTATTCCA
CCTAAGAAACCCAGGAGAATAAGACCACCAGGGGGAGCAAGAGAACCACAGATCCAGGAAGAGGCCCCTA
AGGCCAAGATCCCTGCTCCCAAGATTCCTAGGCCAGCGGTAGAGCCTAGGAGCTGGCACAACCCAGGAGG
AGTCCCAGAGTTTTCCATTGAGAACAGCAGAAGCAGAGAAATGGTTAGGTCTGGAGTCTCCCATGTAGAA
GAGAAGACTTCCAGCAGAGCGAAGGCCTCATCTAGTCCAGGAGGCTGCAACCACCTGAAAAAGGAACGCG
TACCAAGGGAAGCCTCCGGGCTCCCCGCAAACAAGTATCTGCACCCCACGGGCGGAAAAAGTGGAAGTGT
GAGACCCTCAGAGGACAGAAAACGAGCCAGCTCTGCACATACATCCAGCTTGAAAGGGAGTATCCACTCC
GCGGCAGCCAGACTGAGCATGACCGTCTGGAGCAAGATGGCTTGGTCGACACAGCTGGCTAAGCCCCAGC
ACTCAACCCCCTTTCTCACCCCGAGGAATCCTACCCCACTTAACAAAGTGGGGGCTCCACATACAGACGT
GAACTGCAGCCGATTCCCCACTGCTTTAGAGAAGGACCTGGGGCCACCAGGAGTAACTGTTTCTAGGATA
GAGAGCTACCAGGAACATGAGACCCCTGGAAACTCAATGGGGACACCCCACAATCCAGCCATCTCACAAA
AGTTTGGTTTTATGAGGCACTTGAGATATTTTCTCAGACAGTATGGCCTTAAAAAG

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214777 representing NM_001166475
Red=Cloning site Green=Tags(s)

MLLPLLGACAVIGPFQGPEWEPVRGLLSQDQSCKDPRCCGNLLVSCLFLIWQIQQYWHQFSRTRKRNVTK
VPPQRWTVLSAPQDTFVGMIPEFFTHGKNRGLDVHIQQWMQKRRWGYQKTVRQQWDTQYLLSLQKPCQDL
PWDVHTSTEPIFCTSSFSNTCLLPQDSSWEAWQLPWYPRDSQAHPSLAICQRTEQLLDPSHTLVPAEPVS
LRYTSTTLAFSLPNFPPAQSPNVCLREFLPSPLNQQLEMSTKECLEHPQGPLGPWSQTKTVGRECSEATL
QYLNKRKSRREDAQEIRASRAFCPIDSGVEEEEQAKALGYRDQRQERRDTDGEISVSEWERQTQVRTAVR
EQTEKLQRKAQREPGEESSSPQSHIGENQEEFRCKTDIATQIPAWVDQEDAVETPTLGKNEKEARGKEEA
EVEAQGLETQDWTGSKAAENSQGLKWGTRDQFGGNTGTETEVEEGRNKDQIGSGDGVKIETSGRENPGEF
KQQDNEKTQALGWEKQRCIRTENDVQTPAGERGGQSSSENDWKIQASKGENQNLSRQEVELGLKKLREVR
EEDWVVIQAPCWGSQSLRLIAVKRGPCCRDQSQVRGEYTVDISTLESDQREGGDADAANQVTPEAEEQAR
SSEPDMETCIVPSQDEEKTEEENGMYALAQGKRNLRGANGTDGAQTQKLGEENQGQLGNESHKMIHGPKR
KNQKQVRGNDHINNQTSETGNWAELTSKKGDMTHGTGYEEAEEAEGEDGTEGMRIVRGAGGEEGTETKAA
VEESQSPLGDVGTNTHSSELENQKEMGSEDATEARAPEQRNKTEPGNNDIKTQRPESESQGQLAEAGGIG
EQAKGESTSGNGAAEKKNWGEGAGEDCRKLQRPRRKDQRLRLGEVDGKTYQLEWKNQENFRDGNDADIQK
QGKRNPLCFTGDDGSDTRASVGEDQRQSVCETDEKSQTPGQRNQGKRRDTATEIQDVGVQRKRRAEGSKL
SHPSGRGDKGRVGRKDAVRPSPPNDSSGKVGPTRQKCSSAQLASLTSGYGTPGHKQPMAGNGVDSAPGSE
EHLSCQGRAAAWKHRREVSERTQKAQPGSQRRQERDKRVDLGKASSPTCQDPYPQSQASSVFPSLLCPQV
SQAAPAVPCVPVALKTLHKWPALKKSKHLLLESLMKRRIAHLRWGLPRRILESYLLFHFLESCSLPPAGG
RLPGSRTNRERQRHQERHCESQASLLGLESPVGTQSRPVLEKKSSKLCTQVQAVEKRRPTKPEPTGSSIP
PKKPRRIRPPGGAREPQIQEEAPKAKIPAPKIPRPAVEPRSWHNPGGVPEFSIENSRSREMVRSGVSHVE
EKTSSRAKASSSPGGCNHLKKERVPREASGLPANKYLHPTGGKSGSVRPSEDRKRASSAHTSSLKGSIHS
AAARLSMTVWSKMAWSTQLAKPQHSTPFLTPRNPTPLNKVGAPHTDVNCSRFPTALEKDLGPPGVTVSRI
ESYQEHETPGNSMGTPHNPAISQKFGFMRHLRYFLRQYGLKK

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001166475
ORF Size 4536 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001166475.1, NP_001159947.1
RefSeq Size 4673 bp
RefSeq ORF 4539 bp
Locus ID 328573
Cytogenetics 15 E1
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Documents
Resources
"NM_001166475" in other vectors (4)
SKU Description Size Price
MC224637 4930407I10Rik (untagged) - Mouse RIKEN cDNA 4930407I10 gene (4930407I10Rik), (10ug) 10 ug
$1,423.00
MR214777 4930407I10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4930407I10 gene (4930407I10Rik) 10 ug
$1,423.00
MR214777L3 Lenti ORF clone of 4930407I10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4930407I10 gene (4930407I10Rik) 10 ug
$1,723.00
MR214777L4 Lenti ORF clone of 4930407I10Rik (mGFP-tagged) - Mouse RIKEN cDNA 4930407I10 gene (4930407I10Rik) 10 ug
$1,723.00

file-alt Citations

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