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Zfp846 (NM_172919) Mouse Tagged ORF Clone

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SKU
MG214739
Zfp846 (tGFP-tagged) - Mouse zinc finger protein 846 (Zfp846), (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
  $754.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zfp846
Synonyms 2210010B09Rik; 9130221F13; Znf846
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214739 representing NM_172919, codon optimized.
Due to the complexity of NM_172919, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCGCCGACTGTTTGACAAATTGCTATCAGGTGTCTGTTACTTTTGATGACGTGGCTGTCGATTTCA
CGCAGGAAGAGTGGATTCTGTTGGATCAAGCACACCGGGACCTGTACAGAGAAGTGATGCTGGAGAACTA
CCAGAACCTGGCTAGCGCCGGATGCGAAGTGATCAAGCCTTCACTCATCTCTTGGCTGGAGGAGGAAGAT
CTTCACAGAGAGGACCTGCAGGAATGGGAGATGCCCTTGGGAACCCAGGAATCTCCGCTTCATCAGGATT
TCCTGCGCGGACAGACATCAAGCGGAATCCAAACCGGGAGGTCACACAGTGCTCACGAACTGTGCTACTA
CACTCAATGCGGAGAGATTTTTAGCGAGCATAGCTACTTTAAAACACACGTTAAAACACATTCTGCATGG
AACACTGGCCACCTTACTCACTCAGACCACGACGTATCAGCTCAGGTGCACACAATCAAGACCTACCCTT
GCAAGATTTGCGGGAAAGCTTTTGGGCGGAGTTCTAATCTGAACAGGCACCTCCGGTCCCACACCGGAGA
AAAACCTTATGAGTGCAAGGAGTGCGGCAAAGCCTTTACCACATACTCAAGGTTGGTCGAACACTTCCGC
ACACACACAGGGGAGAAACCTTATAAGTGCAAGGATTGTGGTAAAGCCTTTGCCAAACGGTCTGGGCTCA
TAACCCACATCAGCACTCATGCCTCCGAGAAGCCTTTTGCCTGCAAGGAGTGCGGGAAAGCTTTCGCAAG
CAGTCCAAGGCTGTCTCAGCATATCAGAATTCATTCAGGGGAGAGGCCATACATTTGTAAGGAATGCGGG
CGCGCTTTTCTGACGTCCTCCTATCTTAGGAATCATGTTGGCCGGACACATTCAGGTGAGCGGCCTTATA
TTTGCGGGGAGTGTGGCAAGGCTTTTCACTCATATTCTAATCTCAGACGGCATGTGAGGACACACAGTGG
CGAACGACCGTACATTTGCAAGGAATGCGGGAAAGCCTTTCTGAACTCATCATACCTGCATAACCACATC
AGGAAAACGCACAGTGGCGAAATGCCACATATCTGTGGAGAATGCGGAAAAGTCTTTCACGCCTCTTCAT
ATCTGCGCAGACACCTCCGGACTCACTCCGGAGAAAGACCCTGCATTTGCAAGGAGTGCGGAAAAGCTTT
TCTTAATTCAAGCTATCTGAGAAAGCACCTGACTATCCATACTGGCGACAAGCCCTATGAGTGCAAGGAG
TGTGGAAAGGCCTACCGCCGATACAACCTGCTCCACGACCATCTCAAAACTCATGCCGTAGAAAAGCCCT
TTGAGTGCGACGTCTGCGGAAAATCTTTTCAATATTTTTCCTACCTGAACAAGCACATTAGGATCCACAC
AGGGACAAAACCGTACAAGTGTAAGTATTGCGGAAAGGATTTCACCACGTCATCTAGTAGAACAGAGCAC
ATCCGCACTCACACTGGCGAACGGCCATACGAGTGTAGTGAATGCGAAAAGACCTTCACTTCCTCCTCCA
ATCTTATACATCATGTGAAGATCCACGCTAGAGAGAAACCTTTCGTTGAAAACATAGGGAAGCCTACAGT
AGACTTCCCATAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214739 representing NM_172919
Red=Cloning site Green=Tags(s)

MVADCLTNCYQVSVTFDDVAVDFTQEEWILLDQAHRDLYREVMLENYQNLASAGCEVIKPSLISWLEEED
LHREDLQEWEMPLGTQESPLHQDFLRGQTSSGIQTGRSHSAHELCYYTQCGEIFSEHSYFKTHVKTHSAW
NTGHLTHSDHDVSAQVHTIKTYPCKICGKAFGRSSNLNRHLRSHTGEKPYECKECGKAFTTYSRLVEHFR
THTGEKPYKCKDCGKAFAKRSGLITHISTHASEKPFACKECGKAFASSPRLSQHIRIHSGERPYICKECG
RAFLTSSYLRNHVGRTHSGERPYICGECGKAFHSYSNLRRHVRTHSGERPYICKECGKAFLNSSYLHNHI
RKTHSGEMPHICGECGKVFHASSYLRRHLRTHSGERPCICKECGKAFLNSSYLRKHLTIHTGDKPYECKE
CGKAYRRYNLLHDHLKTHAVEKPFECDVCGKSFQYFSYLNKHIRIHTGTKPYKCKYCGKDFTTSSSRTEH
IRTHTGERPYECSECEKTFTSSSNLIHHVKIHAREKPFVENIGKPTVDFPY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172919
ORF Size 1623 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172919.2, NP_766507.2
RefSeq Size 2755 bp
RefSeq ORF 1626 bp
Locus ID 244721
Cytogenetics 9 A3
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Documents
Resources
"NM_172919" in other vectors (2)
SKU Description Size Price
MC218653 Zfp846 (untagged) - Mouse zinc finger protein 846 (Zfp846), (10ug) 10 ug
$555.00
MR214739 Zfp846 (Myc-DDK-tagged) - Mouse zinc finger protein 846 (Zfp846). Note: ORF is codon optimized 10 ug
$554.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.