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Adam20 (NM_001009548) Mouse Tagged ORF Clone

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SKU
MG214728
Adam20 (tGFP-tagged) - Mouse RIKEN cDNA 4930529F22 gene (4930529F22Rik), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $896.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adam20
Synonyms 4930529F22Rik; Adam38
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214728 representing NM_001009548
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGACAAGACAAAGGGCATCCTCCTTTGCTGCCACAGAGGACAACATAGCTATGGATAAGGCTGTGG
TATACACAAGGATACCTCATCTGTACCTCTGGCTAGAGATATTGAATATTCTTTCATCATGGCTCTTGAC
TGGGTATGCTCAGCACACCAGCCTCCCAGAAGTAGTGATACCCTTACGGGTCACAGGCAACAGACCAATG
TGGGCTATGGGATGGCTGACCTATAGCCTGCATTTTGGGGGCCAGAAACATTTTATTCACATAAAGGCCA
AGAAGTTCTTGGTTTCTAGACTCTTCTCTGTATTCACCTACACTAAGCAAGGTGCTCTGCACAAGGACCA
GCCTTATGTCCAGAATGATTGCTACTACCATGGCCATATGGATGGAGACCCAGAGTCTATGGTTGCTATT
ACCACCTGTTATGGAGGCTTTCAAGGAATATTACAGATAAATGGCACAGTCTATGAAATCAAACCCAAGA
ACCTTTCTTCCACATTTGAACATTTGGTTCACAAGATGGACAGTGAGGAGACAGAATTACTCCCCATGAG
ATGTGCATTGACAGAAGAGATAGCAAAGCAAATGAAACGTCAGCAGAATGAGAACCCCACTCTGATGCAA
AACCACTATGAGGAATGGTGGACCCACAGGAGTTTTCTTGACCTGGCATTGGTTGTTGACTGTGAGCGAA
TTCGTTATCATAACAATAACAAATCACACGTGTTAGTGGAAGTATTCTTGATTATCAGTTTAATAAATAA
GATTTATTTTGCACTGGATATTGAGGTAGTTTTAATTGGACTTGAGTTGTGGAATGAAGGAAATCTTGTG
CCCGTGGATAGAATGCAAATTCTTCTAGAGGAATTTTGTGTTTGGAAGACTCTCAGCCTTAATATTCGAA
TTCCAAATGACATTGCACATCTTTTTGTGAATCATTCATTTGGTAAATTCCTTGGTTTAGCCTATGTTGG
TACTGTCTGTCTGCCATCTCATAATTGTGGAGTTGATCGTCTGTTAGGTCCTAATCTTTTTCAATTTGCA
CATATTATAGCACATGAGATGGGTCATAATCTGGGTATGGAGCATGATAGCAGCTCATGTACATGTGGAG
GAATAAATTATTGCTTAATGTCTTCAACGTACAGTTTCAACCCCGAGTTCAGTAACTGTAGTTATTCTAA
CTTCTGGACAACATATGCTACTACCAATTGTTTGCGCAAGGAAAAGATGTCAATAGGCAACCTACAAATC
CAGTTCTGTGGGAATGGAGTTGTTGACGATGGAGAACAGTGTGATTGTGGAGACAGGCATATGTGTGAAA
GAGATCAATGTTGTAACTCCAGGTGTGCCCTTAATGATGGCGCTGCCTGTGCTTTTGGGCTTTGCTGCTT
ATATTGCCAGATCATGCCAGCAGGCACAGTTTGCCGACAAGAGGTCAATGAATGTGATCTTCCAGAATGG
TGCAACGGACATTCACATAAGTGTCCTAATGACGTTTATTTGCTTGATGGGAGTCCCTGTAGAGATGGTG
GCTACTGCTATGAGAAGAGATGTAATAACCGAGATGAACAGTGTAAGCAAATCTTCGGCAAAGAAGCCAG
GAGTGCAGATCACAGTTGCTACAGAGAACTCAACACCCAAGGTGACCGTTTTGGCAACTGTGGTATGATC
AGAGATGCATACTTAAGATGTCATGACCCAGACATCCTCTGTGGCAGAGTTCAATGTGAGAACGTAACAC
GCATTCCATTTTTGAGGGACCATTCCACTGTACACTGGACTCACCTCAATGGTGTCACCTGTTGGGGAAC
TGACTACCATTTTGGGATGACAATCCCTGACATTGGTATTGTGAAAGATGGCACAGACTGTGGTCCAGAG
CATGTGTGTATTAATAAGAAGTGTGTTAGTAAGCCAATTTGGCTAGAACAGTGTTCATCCAAGACATGTA
CTATGAAAGGTGTGTGCAACAATTTACATCACTGCCACTGCAACCGGGGGTGGGACCCACCACATTGCCT
GGAAAGTGGCTTTGGAGGGAGTGTTGACAGTGGCCCTCCACCTGGAGAAGAGGAATCCCAGCAACATATG
GACCTGGTACTGATTACTCCAATTCTTTCCATTGTTTCCTTAGTCTTTCTGTTACCTTGGCTTTTGAATA
GGTATATAAGAACATCATCAAAGTTTGAGGAACCCACTGTTTCCACTTCCAAGGAGGAAGAAGAGAGCCA
TGTGGCAAGTAATGAGTCAGAAGCGCAACAGGTTGAAACA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214728 representing NM_001009548
Red=Cloning site Green=Tags(s)

MQTRQRASSFAATEDNIAMDKAVVYTRIPHLYLWLEILNILSSWLLTGYAQHTSLPEVVIPLRVTGNRPM
WAMGWLTYSLHFGGQKHFIHIKAKKFLVSRLFSVFTYTKQGALHKDQPYVQNDCYYHGHMDGDPESMVAI
TTCYGGFQGILQINGTVYEIKPKNLSSTFEHLVHKMDSEETELLPMRCALTEEIAKQMKRQQNENPTLMQ
NHYEEWWTHRSFLDLALVVDCERIRYHNNNKSHVLVEVFLIISLINKIYFALDIEVVLIGLELWNEGNLV
PVDRMQILLEEFCVWKTLSLNIRIPNDIAHLFVNHSFGKFLGLAYVGTVCLPSHNCGVDRLLGPNLFQFA
HIIAHEMGHNLGMEHDSSSCTCGGINYCLMSSTYSFNPEFSNCSYSNFWTTYATTNCLRKEKMSIGNLQI
QFCGNGVVDDGEQCDCGDRHMCERDQCCNSRCALNDGAACAFGLCCLYCQIMPAGTVCRQEVNECDLPEW
CNGHSHKCPNDVYLLDGSPCRDGGYCYEKRCNNRDEQCKQIFGKEARSADHSCYRELNTQGDRFGNCGMI
RDAYLRCHDPDILCGRVQCENVTRIPFLRDHSTVHWTHLNGVTCWGTDYHFGMTIPDIGIVKDGTDCGPE
HVCINKKCVSKPIWLEQCSSKTCTMKGVCNNLHHCHCNRGWDPPHCLESGFGGSVDSGPPPGEEESQQHM
DLVLITPILSIVSLVFLLPWLLNRYIRTSSKFEEPTVSTSKEEEESHVASNESEAQQVET

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001009548
ORF Size 2280 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001009548.2, NP_001009548.1
RefSeq Size 2589 bp
RefSeq ORF 2283 bp
Locus ID 384806
Cytogenetics 8 A4
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Documents
Resources
"NM_001009548" in other vectors (4)
SKU Description Size Price
MC221412 Adam20 (untagged) - Mouse RIKEN cDNA 4930529F22 gene (4930529F22Rik), (10ug) 10 ug
$766.00
MR214728 Adam20 (Myc-DDK-tagged) - Mouse RIKEN cDNA 4930529F22 gene (4930529F22Rik) 10 ug
$589.00 $696.00
MR214728L3 Lenti ORF clone of Adam20 (Myc-DDK-tagged) - Mouse RIKEN cDNA 4930529F22 gene (4930529F22Rik) 10 ug
$996.00
MR214728L4 Lenti ORF clone of Adam20 (mGFP-tagged) - Mouse RIKEN cDNA 4930529F22 gene (4930529F22Rik) 10 ug
$996.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.